貴州幾種野生魚類染色體組型與酯酶同工酶研究.pdf

1、貴州大學(xué)碩士學(xué)位論文貴州幾種野生魚類染色體組型與酯酶同工酶研究姓名：楊曉芬申請學(xué)位級別：碩士專業(yè)：動物遺傳育種與繁殖指導(dǎo)教師：張小雪20070401貴州大學(xué)2007屆碩士論文AbstractInthisstudy，thechromosomek鄴，o哆peofseveralwildfishincludingAcmssocheillusiridescene，ErythroculterilishaeformisCtenopharyngodon

2、idellu吖Cuvier&Valenciennes)，HemicculterLeuciclus(Basilewaky)inGuizhouw骶investigatedbymeansofobtainingchromosome研3ecimeil$frommetaphaseofkidneycellbyvivoinjectionofPHAandColohicine。hypatoicairdryingandGiemsastaining，respe

3、ctivelyTheresultswereasfollows：1ThediploidchromosomenumberofAcmssocheillusiridescenswas2n250，anditskaryotypefomularwas14m16sm10st10t，NF=802ThediploidchromosomenmberofErythroculterilishaeformiswas2n=48anditskaryotypefomul

4、arwas16m26sm6st，NF903ThediploidchromosomenumberofCtenopharyngodonidellus(Cuvier&Valenciennes)was2n=48，anditskaryotypefomularwasl8m22smSst，NF=88t耵圮diploidchromosomenumberofHemicculterLeuciclus(Basilewaky)was2n=48。anditska

5、ryotypefomularwas16m22sm6st6NF=90TheesteraseisozymesinfiverandkidneyofGulzhonPelteobagrusfulvdrace(Richardson)，Ameiurusnebulosus，Ctenopharyngodon娩珈吖Cuvier&Valenciennes)，CarassiusauratusandErythroculterilishaeformiswerean

6、alyzedbymean8ofVerticalpolyacrylamidegelelectrophoresisTheresultsindicatedthat：ncliverandkidneytissueESTofthesefivefishshowedobvioustissuespecifici哆Theresultsofclassificationofenzymesforthecharacters，jointcoefficientcalc

7、ulationandtheaverageclassclusteranalysiswereconsistentwiththeIraditionulmorphologicalclassificationTheresultsoftheinvestigationwillprovidevaluablegeneticandbiochemicalfundamentaldataforthegeneticdiversityanalysis，thefurt