Analysis of Temperature Effects on Rice Flowering and Yield Traits Through QTL Approach and Identification of a Novel Rice Plan.pdf

1、AnalysisoftemPeratureeffeetsonricetraitsthroughQTLfloweringandyieldaPProaehandidentifleationofanovelPlantheightgenerlCeHuazhongAgrieulturalUniversityMaj:BioehemistryMoleeularBiologySubjeet:RieeMoleeularBiologyName:KoviMa

2、llikarjunaRaoAdvisor:DrXingYongzhongDegree:DoetorofPhilosoPhyMembersofguidinggrouP:Dr.XingYOngzhongDr.XieConghuaDr.LiuJunDLYtzSibinDr.HeYuqingDateofsubmission:Deeember，2010Dateofreeeivingeertineate:December，2010Collegeof

3、LifeSeieneeandTechn0logyDeeembef，2010HuazhongAgrieulttlralUnlversltyPhDDissertatio一12010ChaptcrHl.......................................................................................................................……47

4、31ntroducti0n............................................................................................................……473.1MaterialsandMethods..........................................................……，............

5、...........……493.1.1Plantmaterialandfieldexperimentaldesign...............................................……493.1.2Growthchamberconditionsftheparents.................……，.........................……493.1.3Traitmeasurements

6、......................................................................................……503.1.4DNAisolationandpolymorphicscreening..................................................……503.1.5Sequencingphotoperiodgene......

7、........................................……，.................……503.1.6Dataanalysis................................................................................................……503.2Results.............................

8、....................................................................................……513.2.1PhotoPeriodandtemPeraturevariationsamongthreeseasons....................……513.2.2Traitsresponsetodifferentseasons..............

9、...............................................……523.2.3Heritabilityandcorrelation..........................................................................……543.2.4GxEinteraction.........................................

10、.................................................……553.2.5Developmentofgenetichnkagemaps.........................................................……553.2.6QTLanalysisbysinglemarkeranalysisandeomPositeintervalmaPPing..……56

11、3.2.7Q‘Einteractions........................................................................................……583.3Dlscussion...............................................................................................

12、............……593.3.1TraitPerfmaneewasmainlyinfluencedbytemPerature...........................……593.3.2AmajPleiotroPieQTLstablyexPressedindiverseenvironments...........……603.3.3IrnPortaneeofenvironmentaleffeetandGXEingen

13、etiebasesofyieldrelatedtraits...........................................................................................................……603.4References...................................................................

14、........................................……61ChaPterIV.........................................................................................................................……6641ntroduction.......................……，...

15、............................................................................……664.1.MaterialsandMethods.......................................................................................……674.1.1Plantmaterialsanddeve

16、loPmentofmaPPingPoPulation.，.，........................……674.1.2FinemaPpingpopulation..............................................................................……684.1.3DNAextractionandmarkerselecti0n..................

17、........................................……684.1.4Plantheightandheadingdatemeasurement................................................……684.1.5BulksegregantanalysisfPrimarymapping..........................................

18、....……694.1.6Genetichnkagemapandgeneticeffectanalysis.........................................……694.1.7SequeneinganalysisofsdlgeneandProbableeandidategene...................……694l8RNAeXtraetio，1quantitativerealtimePCRana

19、lysis(qRT一PCR).........……694.2.Results................................................……，........................................................……704.2.1PhenotyPievariationsofPlantheightinBC4FZPoPulationallditsProgeny.…