三種生物濾器載體生物膜中細菌的數(shù)量動態(tài)及其代謝活性.pdf_第1頁
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1、大連水產(chǎn)學院碩士學位論文三種生物濾器載體生物膜中細菌的數(shù)量動態(tài)及其代謝活性姓名：邵華申請學位級別：碩士專業(yè)：水產(chǎn)養(yǎng)殖指導教師：馬悅欣20050601ThenumbervariationandmetabolicactivitiesofbacteriainbiofilmsassociatedwiththreedifferentkindsofbiofiltersAbstract：Thedeterminationofthenumbervaria

2、tionandmetabolicactivitiesofbacteriainbiofilmsassociatedwiththreedifferentkindsofbioflltersweredoneinthisdissertationFirstlythenumbervariationofheterotrophicbacteriaandammonificationinbiofilmsassociatedwithsubmergedbiofi

3、hersweredeterminedTheresultsshowedthatthenumbersofheterotrophicbacteriadeclinedwiththeformationofbiofilm，Aftermaturationofbiofilm，theammonificationratesofbothzeoliteandnonwovenclothcarriersaveraged1745“g／(cm3h)；26ng，(gh)

4、and1741ug／(cm3h)；999／(gh)within24hSecondlythenumbervariationandmetabolicactivitiesofheterotrophicbacteria，ammonifiers，ammoniaoxidizingbacteriaandthemetabolicactivitiesofpurestrainsofheterotrophicbacteriaandanxmonifiersin

5、theNereisclose—recirculatingculturesystemweredeterminedTheresultsshowthatthenumbersofthreephysiologicalgroupsofbacteriaincreasedgraduallywiththeformingofbiolfilm，andbothkeptstableaftermaturationofbiofilm111einfluencesofs

6、ubstrateconcentrationstemperatureandpHonmetabolicactivitiesofbacteriawereobviousEightstrainsofheterotrophicbacteriaandsevenstrainsofammonifierswereisolatedfromthebiofilmsM：eantimethebiofilmsassociatedwithsandswereobserve

7、dbyscanningelectronmicroscope，theshapesofbacteriawerevariousTheoxygenuptakeratesofheterotrophicbacteriaisolateswere1088O73～114985062g／(mgprotein‘d)，theammonificationratesofammonifierisolateswere076O57～147109g／(mgprotein。

8、d)Finallythenumbersandmetabolicactivitiesofheterotrophicbacteria，ammonifiers，ammoniaoxidizingbacteria，nitriteoxidizingbacteriaandthemetabolicactivitiesofpurestrainsofheterotrophicbacteriaandammonifiersinbiofilmsassociate

9、dwithcarriersinfluidizedbedintherecirculatingculturesystemofParalichthysolivaceusweredeterminedTheresultsshowthatthenumbersandthemetabolicactivitiesofthefourphysiologicalgroupsofbacteriakeptstableaftermaturationofbiofilm

10、TheinfluencesofsubstrateconcentrationstemperatureandpHonmetabolicactivitiesofbacteriawereobviousMeantimethebiofilmsassociatedwithsandswereobservedbyscanningelectronmicroscope，theshapesofbacteriawerevariousTwelvestrainsof

11、heterotrophicbacteriaandsixstrainsofammonifierswereisolatedfromthebiofilmsTheoxygenuptakeratesofheterotrophicbacteriaisolateswere8644099～3489151g／(mgproteind)，theammonificationratesofammonifierisolateswere0669___002～2377

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