Th1細胞因子的含量對羊胚胎移植受胎率的影響及中藥提高受胎率的機理研究.pdf

1、河北農(nóng)業(yè)大學碩士學位論文Th1細胞因子的含量對羊胚胎移植受胎率的影響及中藥提高受胎率的機理研究姓名：苗玉青申請學位級別：碩士專業(yè)：臨床獸醫(yī)學指導教師：李鐵拴20050612StudyonthecontentofThlcytoldnesaffectingfecundationrateofeweontheembryotransplantationandthemechanismofherbalmedicineincreasingfecunda

2、tionrateAbstractGraduateSmdem：MiaoYuqingMajor：ClinicalVeterinaryAdvisor：Liq]eshuanThisstudywasdesignedtodiscussthecorrelationbetweenthecontentof1111cytokinesandthefecundationrateontheembryotransplantation，researchthefunc

3、tionofnitricoxide(NO)intheembryoimplantationandgrowth，andexplainthemechanismofherbalmedicine“AnTaiSan”O(jiān)ilpromotingthefecundationrateInthisexperiment，basedonembryotransfertechnique，血eanthorscollectedplasmanduterinemilkfro

4、mdonorsandreceiptsatthetimeofembryotransplantingandthereceipts“plasmwascollectedaftertwoeStlOUScyclesThecontentofcytokineswasmeasuredbyELlSAandthecontentofNOwasmeasuredthroughthemethodofnitricdeoxidizeenzymeTheresultswer

5、ethatthecontentofThlcytokinessuchas11NF—a、狂7NnIL一2inplasmoruterineIIlilkofgoatsandsheepwhowereestrouswassignificantlyhigherthanthenormalpregnantgoatandsheep(PO05)11lisindicatedthatitsadverseforembryoimplantationandgrowth

6、tosecretedThlcytokinessuperfluouslyItwasimportantforembryogrowthaftertransplantationtokeepthereceipts、uterusimmuneconditionconformingtothedonorsnecontentof伯1cytokinesinplasmofpregnantgoataftertwoesllouscycleswaslowerthan

7、thatatthetimeofWansplantingButthedefferentwash’tsignificant(P005)ThesefindingsdemonstratedthatthecontentofThlcytokinesdecreasedwiththedevelopmentofpregnancywhichisgoodtopregnancyThecontentofNOinplasmofgoatandsheepwhowere

8、estrouswassignificantlyhigherthanthenormalpregnantgoatandsheepfP005)111isresultshowedsuperfluousNOmaydoharmtoembryos，andledtoearlierembryolossAtthetimeofembryotransplanting，thechangingtendencyofthecontentofNOwasconformed

9、ton1cytokinesinplasmofgoatreceiptsnisresultdemonstratedtherewasacorrelationbetweenNoandThlcytokines，F(xiàn)urthermorewefoundtheNOcontentofpregnantewesonpregnant40dayswassignificantlyhigherthanthetransfertime(PO05)，butwhichwass

10、ignificantlylowerthanestrousewesatthesametime(PO05)ThecontentofNOincreasedwiththedevelopmentofpregnantinordertomeettheneedsofembryogrowthAfterembryotransplanting，20goatreceiptswererandomlydividedintotwogroups，includinghe