豬囊尾蚴的發(fā)育生物學(xué)及藥物作用機(jī)理.pdf

1、東北農(nóng)業(yè)大學(xué)博士學(xué)位論文豬囊尾蚴的發(fā)育生物學(xué)及藥物作用機(jī)理姓名：劉永杰申請學(xué)位級(jí)別：博士專業(yè)：基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師：李慶章2000.5.1劉水杰英文摘要2000年5月DEVELOPMENTALBIOLOGYOFCYSTICERCUSCELLULOSAEAND枷!CHANISMOFACTIONOFDRUGSAbstractTsoliumcysticercosisisanimportantparasiticzoonosisendangerin

2、gthedevelopmentofhusbandryandhealthyofhumanInordertoselectaneffectivemedicineagainstcysticercosisandtoprovideatheoretica]basisforchemotherapy，theauthorstudiedfirstlyandsystematicallYdevelopmentofcYstlcerciinvivoandinvitr

3、oandtheeffectofdrugsoncysticerciindlfferentdevelopmentalstagesByoptica]microscopeandelectronicmicroscopethemorphologyandstructureofcystieerciwereobservedindetailhtechnique，insituend一1abelling，wasappliedtodetectqualitativ

4、elyandquantitativelytheapoptotlceellpositiveindex(AI)incysticerciandhostsIncombinationwiththedetectionofthe1evelsofnitricoxide(NO)andAIoflymphocyte(L—AI)，theclinalgenesisofcysticercosisandpossiblemechanismofactionofthedr

5、ugsagainstcysticerciwereanalyzedTheresultswereshowedasf0110ws(1)Aninvitroculturesystem1ncorporatingacellmonolayerwithoutgasbhasewasfirstlYusedtoinducemostoftheoncospheresofTsoliumtoenterDOstoncosperaldevelopmentThe1arval

6、cansurvivefor16days(2)InvitrohatchedoncosphereswereinjectedintravenouslYintothemiceThemjcewereautopsied2monthsafterinocufationCysticerciwerefoundinthe1ungand56percentmicewereinfectedThemiceinfectedintravenouslYbyoncosphe

7、resarevaluableexperimentalanima]modelsforstudyingTs01iumCystlCercosis(3)Changesofmorph0109y，hist0109icalstructureandultrastructureofCcel1ulosaeinthedevelopmentwerefirstiYandsystematicallYstudiedThesizeofcysticerciinthesa

8、mehostwerevariab]eTheyoungestcysticerciobservedwerei9days01d，butsuckersandhookswerenotfoundinSC01exregionHistologyshowedthatalotofcellsaccumulatedatoneofthepolesandattheprollferationregionthetegumentinvaginatedWiththedur

9、ationofinfection，scolexbegantodifferentiateandpossessedsuckersandhooksDuringthedevelopmentthebladderwallisatransitoryembryoonthebasisofwhichtheSC01exdevelops60days】ater，mostofcysticerciwerematureTheultrastructureshowedth

10、ecomponentsofcysticerciincreasedinnumberandextentbutnonewcellsortisssuesappearedasthecysticercigrewBladderwallandsoolexhadsomedifferencesinultrastructuresThebladderwallseemstohavedifferentfunctionsfromSCOlexThebladderwal