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簡(jiǎn)介:武漢理工大學(xué)碩士學(xué)位論文哲學(xué)視角下的綠色消費(fèi)研究姓名劉瑞申請(qǐng)學(xué)位級(jí)別碩士專業(yè)科學(xué)技術(shù)哲學(xué)指導(dǎo)教師彭攀20080401武漢理工大學(xué)碩士學(xué)位論文ABSTRACTWITHTHERAPIDDEVELOPMENTOFINDUSTRIALCIVILIZATIONTHELEVELOFPRODUCTIVEFORCESHADCONTINUOUSIMPROVEMENTANDSOCIALWEALTHINCREASEDRAPIDLYDOMINATEDBYTHETHINKINGOFNARROWHUMANCENTEREDDOCTRINE,CONSUMERISMVALUESANDLIFESTYLEBASEDONMATERIALDOCTRINEAROSEANDEVENTUALLYCAUSEDTHESERIOUSDAINAGETONATLII詛RESOURCESANDECOLOGICALENVIRONMENTSOHUMANBEINGSANDALLLIVINGORGANISMSONEARTHALEFACINGASEVERESURVIVALPRESSURENOWDURINGTHELATE1970SANDEARLY1980S,ALARGESCALEGREENMOVEMENTBROKEOUTINTHEWESTERNWORLDGREENCONSUMPTIONASAEONSUMPTIONADVOCATEOFGREENENVIRONMENTALPROTECTIONORGANIZATIONBECAMEWELLKNOWNWITHTHEDEVELOPINGGREENMOVEMENTANDGRADUALLYDEVELOPEDINTOANEWFASHIONOFTHEINTERNATIONALCONSUMPTIONINTHE1990SGLEENCONSUMPTIONWITHTHEDEEPENINGECOLOGICALCRISISANDCHANGINGCONCEPTOFHUMANCONSUMPTIONISATOTALLYNEWLIFECONCEPTANDCONSUMPTIONPATTERNBASEDONTHEREFLECTIONOFTHEDEFECTSOFTRADITIONALONESITISTHESENSEOFCRISISANDRESPONSIBILITYACCORDINGTOVARIOUSECOLOGICALPROBLEMSINVARIOUSDEGREESITISTHEECOLOGICALSTUDYANDPHILOSOPHYREFLECTIONOFTRADITIONALCONSUMPTIONPATTERNSANDITISANEWCONCEPTOFCONSUMPTIONPATTERNSANDVALUESFORMEDINTHEPRACTICEOFHUMANACTIVITIESTHEULTIMATECONCELTLOFGREENCONSUMPTIONISSAVINGTHENATUREFROMTHEDAMAGEOFHUMANBEINGSITISHOPEDTHATTHROUGHTHEPROMOTIONOFGREENCONSUMPTIONWECANCHANGETHESTATUSOFECOLOGICALENVIRONMENTANDPROMOTETHESUSTAINABLEDEVELOPMENTOFHUMANSOCIETYITISAMODEMCONSUMPTIONPATTERNGUIDEDBYTHETHINKINGOFSUSTAINABLEDEVELOPMENTGREENCONSUMPTIONISBENEFICIALNOTONLYTOPROTECTINGTHEECOLOGICALENVIRONMENTBUTALSOTOTHEHEALTHYDEVELOPMENTOFHUMANANDSOCIETYITISTHERESULTOFRATIONALCHOICEOFHUMANBEINGANDMORALSELFDISCIPLINEANDITISANECESSARYREQUIREMENTOFECOLOGICALCIVILIZATIONW弛GRADLLALLYFURTHERUNDERSTANDINGOFHUMANBEINGS,THEMEANING”GREEN”HASBEENFARBEYONDTHESCOPEOFENVIRONMENTALPROTECTIONTHE”GREENCONSUMPTION”GRAD砌LYHASTURNEDITSFOCUSTOTHEREVIEWOFINDUSTRIALCIVILIZATIONTHEREFLECTIONOFSOCIALDEVELOPMENTMODEL,ASWELLASTHETHINKINGOFSURVIVALSHAPEINTHEFUTURE,N
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簡(jiǎn)介:Y1409592蠶他天晉高等學(xué)校教師碩士學(xué)位論文2008屆發(fā)展哲學(xué)視閹中的中國(guó)農(nóng)村城鎮(zhèn)化發(fā)展模式以改革開放三十年采的蘇州為倒ONTHELOGICALANDTHEHISTORY一一REALITYCHANGESINDEVELOPMENTMODEOFRURALURBANIZATIONFROMNILANGLEOFDEVELOPMENTPHILOSOPHY1址ETHESUZHOUASAEXAMPLE研究生姓名指導(dǎo)教師姓名專業(yè)學(xué)位名稱研究方向論文提交日期杭雪花陳忠馬克思主義哲學(xué)發(fā)展哲學(xué)2008年9月發(fā)展哲學(xué)視閾中的中國(guó)農(nóng)村城鎮(zhèn)化發(fā)展模式以改革開放三十年來(lái)的蘇州為例英文摘要ONTHELOGICALANDTHEHISTORYREALITYCHANGESINDEVELOPMENTMODEOFRURALURBANIZATIONFROMANANGLEOFDEVELOPMENTPHILOSOPHYTAKETHESUZHOUASAEXAMPLEABSTRACTCURRENTLYURBANIZATIONISBECOMINGAHEATEDISSUEINOURCOUNTRYIN2000,STAGIEST,ANAMERICANECONOMISTS,WINNEROFNOBELECONOMICSPRIZEONCESAIDTWOTHINGSPROFOUNDLYIMPACTTHETWENTYFIRSTCENTURYHUMANSOCIETYPROCESS,THEFIRSTBEINGTHENEWTECHNOLOGICALREVOLUTIONHEADEDBYTHEUSA,ANDTHESECONDISTHECHINESEURBANIZATIONGIGANTICACHIEVEMENTSHAVEBEENMADEINCHINESEURBANIZATIONSINCEITSREFORMANDOPENINGUPWHICHBEGAN30YEARSAGOHOWEVERCHINAHASALSOENCOUNTEREDAVARIETYOFDIFFICULTIESATPRESENTALONGTHISROADINOURVIEW,THEROUTECHOICEORTHEDEVELOPMENTPATTERNISTHEKEYPROBLEMINCHINESERURALURBANIZATIONSTATINGFROMANANGLEOFDEVELOPMENTPHILOSOPHYTHEPAPERSTUDIESTHELOGICALANDTHEHISTORYREALITYCHANGESINDEVELOPMENTMODEOFRURALURBANIZATIONINTHEPAST30YEARSOFREFORMANDOPENINGUPITBASESITSRESEARCHONTHEDEVELOPMENTPROCESSOFSUZHOUCITYAMINIATURETHATPROFOUNDLYREFLECTSTHESEVERALSTAGESINCHINESERURALURBANIZATIONVILLAGECIVILIZATIONDOMINATEDSTAGE,CITYCIVILIZATIONDOMINATEDSTAGEANDTHEFORTHCOMINGSPACECIVILIZATIONSTAGE,ONETHATPUTFORWARDBYTHEPAPERTHEAUTHORALSOEXPLORESTHECONTEMPORARYREASONABLITYANDPOSSIBLECHOICEOFTHEDEVELOPMENTMODEKEYWORDSURBANIZATIONDEVELOPMENTMODESPACECIVILIZATIONⅥ砸TTENBYHANGXUEHUASUPERVISEDBYCHENZHONGII
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簡(jiǎn)介:蠶悄式誓高等學(xué)校教師碩士學(xué)位論文2008屆關(guān)于有效實(shí)現(xiàn)個(gè)體領(lǐng)導(dǎo)力的哲學(xué)思考PHILOSOPHICRENECTIONONACHIEVILLGINDIVIDUALLEADERSHIPE施CTIVELY研究生姓名指導(dǎo)教師姓名專業(yè)學(xué)位名稱研究方向論文提交日期尚久悅陳向?yàn)戱R克思主義哲學(xué)領(lǐng)導(dǎo)哲學(xué)2008年LO月關(guān)于有效實(shí)現(xiàn)個(gè)體領(lǐng)導(dǎo)力的哲學(xué)思考英文摘要PHILOSOPHICREFLECTIONONACHIEVINGINDIVIDUALLEADERSHIPEFIFECTIVELYABSTRACTN’SAHOTISSUETOSTUDYTHELEADERSMPINACADEMICCIRCLESALLDINDUSTRIALCIRCLESINRECENTYEARSTHEINDIVIDUALLEADERSHIPISTHEBASEOFTE鋤LEADERSHIPA11DORG砌ZATIONLEADERSHIP,ITISTHEBASICELEMENTOFTHET11REELEVELSOFLEADERSLLIPACHIEVINGINDIVIDUALLEADERSHIPEAECTIVELYREFERSTOTHATT11ROUGHEXERCISINGPO、VERCORRECTLY,TLLELEADER印PLIESTHELEADINGABILIT,AILDCHA衄TOINNUENCETHEMASSES,AILDDEVELOPREMARKABLELEADERACTIVIT,TEMM,ETHESU巧ECTIVEANDOBJECTIVEWORLDTOGETHERBUTINREALI饑THEINDIVIDUALLEADERSLLIPISFACINGMECON打ADICTIONOFT、VISTINGTHEPO、ⅣEROUTLOOK,LACKOFINDIVIDUALCHANNINGAJLDTHEINVALIDOFLEADER矗MCTION,、VHICHRESULTSINTHEINE髓CTIVEACLLIEVEMENTOFINDIVIDUALLEADERSLLIPINORDERTOSOLVETHEPROBLEM,TTLELEADERSHOULDFOMCORRECTCONCEPTOFTHEACMEVEMENTSI1111ISOMCIALCAREER,TOTRAINTHEQUALITYOFMQ,EQ,LQALLDRQ,AILDDEVELOPTLLEABILIT,OFSTUDY,INNOVATIONANDEXECUTION7MSISTLLEBASICCONDITIONOFACHIEVINGINDIVIDUALLEADERSHIPE虢CTIVELY,ISTHEINTEMALCAUSETHEENVIROIULLENTISMEOUTERPOWEROFRESTRICTINGIILDIVIDUALLEADERSHIP,ITISNECESSARYTOCREATEGOODLEADERENVIROMENTTOENSURETHEACHIEVEMENTOFINDIVIDUAL1EADERSHIP,ITISTHEEXTEMALCAUSED謝NGTHELEADERSMPPMCTICE,TLLE1EADERSHOULDIMPROVEMESTANDAIDOFSCIENTIFICDECISION,RECOGLLIZEDIALECTICALLYANDSOLVEMECONTRADICTIONOFMAKINGUSEOFPERSOIMEL,ANDSTIMULATEMECONSCIOUSACTIVITROFTHEMASSESTLLISISTHEBASIC、VAYTOACLLIEVEINDIVIDUALLEADERSHIPE虢CTIVELYONLYINTHISWAYCANTHELEADERINTE掣ATETHELEADINGPO、№R’ABILI壩CHARMINGAILDFORCETOTHEDEVELOPMENTOFUNDERTAHNGANDT11EPRO伊ESSOFTLLESOCIE夠KEYWORDSINDIVID吼LEADERSHJPLEADERFORCELEADERQUAL時(shí)LEADERENVIRO姍ENTLEADERFUNCTIONIIWRITTENBYSHALLGJIUYUESUPEN,ISEDBYCHENXIANGL鋤
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簡(jiǎn)介:中圖分類號(hào)UDC學(xué)校代碼10055密級(jí)公開高腳犬謦博士學(xué)位論文國(guó)際娃濟(jì)新秩序與中國(guó)對(duì)非洲直接投資戰(zhàn)略研究融舞裟1MEC鰍ON?OMICOS姚RDE把R咖ANDS慨TUDYA論文作者申請(qǐng)學(xué)位學(xué)科專業(yè)答辯委員崔荻經(jīng)濟(jì)學(xué)博士政治經(jīng)濟(jì)學(xué)K~E席羅肇鴻指導(dǎo)教師張仁德教授培養(yǎng)單位經(jīng)濟(jì)學(xué)院研究方向比較經(jīng)濟(jì)體制評(píng)閱人羅肇鴻冒天啟賈根良何自立南開大學(xué)研究生院二OO九年十二月南開大學(xué)學(xué)位論文原創(chuàng)性聲明本人鄭重聲明所呈交的學(xué)位論文,是本人在導(dǎo)師指導(dǎo)下進(jìn)行研究工作所取得的研究成果。I除文中已經(jīng)注明引用的內(nèi)容外,本學(xué)位論文的研究成果不包含任何他人創(chuàng)作的、邑公開發(fā)表或者沒(méi)有公開發(fā)表的作品的內(nèi)容。對(duì)本論文所涉及的研究工作做出貢獻(xiàn)利其他個(gè)人和集體,均已在文中以明確方式標(biāo)明。本學(xué)位論文原創(chuàng)性聲明的法律責(zé)倒由本人承擔(dān)。學(xué)位論文怍者簽名崔苤2009年12月28日一‘‘。。‘‘一一。‘‘一4一‘‘。一一一一一‘一‘‘‘’一一‘’一‘’‘‘‘。一一’一’?!?。’‘。一一一‘’‘‘一‘‘?‘‘一?!弧弧枪_學(xué)位論文標(biāo)注說(shuō)明根據(jù)南開次學(xué)有關(guān)規(guī)定,非公開學(xué)位論文須經(jīng)指導(dǎo)教師同意、作者本人申請(qǐng)和相關(guān)部門批準(zhǔn)劈能標(biāo)注。未經(jīng)批準(zhǔn)的均為公開學(xué)位論文,公開學(xué)位論文本說(shuō)明為空白。論文題目{申請(qǐng)密級(jí)}口限制≤2年口秘密≤10年口機(jī)密≤20年保密期限I20年月日至20年月日審批表編號(hào)1批準(zhǔn)日期20年月日限制★2年最長(zhǎng)2年,可少于2年秘密★L(fēng)O年最長(zhǎng)5年,可少于5年機(jī)密★20年最長(zhǎng)10年,可少于10年
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簡(jiǎn)介:西南農(nóng)業(yè)大學(xué)碩士學(xué)位論文非洲菊GERBERAJAMESONIIBOLUS組織培養(yǎng)快繁體系的研究姓名鄭秀芳申請(qǐng)學(xué)位級(jí)別碩士專業(yè)植物學(xué)指導(dǎo)教師李名揚(yáng)20000401TISSUECULTUREANDRAPIDPROPAGATIONOFGERBERAJAMESONIIBOLUSCANDIDATEZHENGXIUFANGADVISORPROFLIMINGYANGCENTEROFBIOTECHNOLOGYSWAU,CHONGQINGABSTRAETINTHISSTUDYTHERULEOFORGANSFORMATIONDURINGGERBERAJAMESONBOLUSCULTURED/NVITROWASINVESTIGATEDONPOINTSOFGENOTYPES,HORMONESCOMBINATION,BASALMEDIUMANDSUCROSECONCENTRATIONETCTHEMETHODSANDTECHNOLOGIESOFRAPIDPROPAGATION,PROPAGATIONEFFICIENCYANDGENETICSTABILITYOFREGENERATEDPLANTLETSWEREALSOPROBEDTHERESULTSINDICATEDTHATYOUNGCAPITULUMWASO塒MULNEXPLUNTSFORCULTUREALTHOUGHTHERE’SDIFFERENCEAMONGVARIETIES,ADVENTITIOUSSHOOTCOULDBEINDUCEDDHCⅡYFROMEXCISEDCAPITULUMOFGERBERAJAMESONIIBOLUSINTWOMONTHSFORINDUCTIONOFTHEADVENTITIOUSBUD,EXTRAHORMONESWASNECESSARY,THEOPTIMUMHORMONESCOMBINATIONWASO1MG/LIAAAND10MGLBA;1%SUCROSEWASBETTERFORTHEBUD’SDIFFERENTIATIONTHANTHATOFHIGHERCONCENTRATION;AMONGBASALMEDIUM,B5VCASTHEBESTFOLLOWEDBY1/2MS,MSINTHEPROCESSOFMULTIPLICATIONYOUNGBUDPROPAGATIONBYAXILLARYBRANCHINGTHEADDITIONOFOIMG/LIAAANDIMG/LBATOMSMEDIUMWASFOUNDTOBETHEBESTCOMBINATIONFORTHESHOOTSPROPAGATIONOFGERBERAJAMESONIIBOLUSAFTERSINGLESHOOTSWERETRANSFEREDONTOTHEMULTIPLIEATIONMEDIUM,ALLOFTHEFOURVARIETIESCOULDSUCCESSIVEMULTIPLIEATE,57TIMESPROLIFERATINGRATECANBEMAINTAINEDPER20DAY;ABOVE5MG/LBAANDUNDER3%SUCROSEINTHEMULTIPLICATIONMEDIUMWEREUNFAVOURABLETOPROLIFERATIONOFSHOOTSEFFECTSOFROOTINGONDIFFERENTMEDIUMWERETHAT1/2MSSUPERIORTO1/4MSANDMS,IBAWASBETTERTHANNAA1/2MSSUPPLEMENTEDWITHIBAO01MG/LGAVETHE2
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簡(jiǎn)介:華南師范大學(xué)碩士學(xué)位論文非洲菊(GERBERAHYBRIDA)耐熱變異品系的離體篩選與初步鑒定姓名黃志剛申請(qǐng)學(xué)位級(jí)別碩士專業(yè)植物學(xué)指導(dǎo)教師陳兆平王小菁20040601華南師范大學(xué)碩士學(xué)位論文非洲菊耐熱變異的離體篩選與初步鑒定4.SR7、SHP5、SE6初選系組培苗在模擬高溫下與對(duì)照的耐熱性進(jìn)行了比較,發(fā)現(xiàn)它們?cè)?5℃和35℃兩種高溫處理下的存活率都有所提高。其中,SR7與對(duì)照在35℃處理15D時(shí)的存活率具差異顯著,是一個(gè)耐熱變異新材料。SR7的PRO含量、POD和SOD活性等指標(biāo)在35℃處理后與對(duì)照S5具有顯著的差異,可能與非洲菊耐熱性的提高有關(guān)。我們的研究結(jié)果表明,通過(guò)體細(xì)胞無(wú)性系變異離體篩選技術(shù)獲得目的變異是切實(shí)可行的,但需建立在大規(guī)模篩選的基礎(chǔ)之上。關(guān)鍵詞非洲菊,耐熱性,離體篩選,存活率,變異系V
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簡(jiǎn)介:|裟墜0200;燃’象霧幽東牡嶧鬣失孽博士學(xué)位論文文1七積角下的稻盛經(jīng)營(yíng)哲學(xué)與企業(yè)實(shí)踐一K???1‘M1D岬口LPH№”PHY8“DE“T唧㈧IT脅1IH。C”1T州NGD作者鐘放指導(dǎo)教師尚俠教授學(xué)科N日語(yǔ)語(yǔ)言文學(xué)Ⅲ蔸方向日本文化東北師范大學(xué)學(xué)位評(píng)定委員會(huì)2008年12月到經(jīng)營(yíng)者與客戶、顧客、供應(yīng)商的關(guān)系;共生循環(huán)除涵蓋了前兩個(gè)概念所涉及的關(guān)系外,更多地是調(diào)節(jié)宏觀上企業(yè)與自然界的關(guān)系、企業(yè)與競(jìng)爭(zhēng)對(duì)手的關(guān)系乃至國(guó)際關(guān)系。這三個(gè)概念調(diào)節(jié)的社會(huì)關(guān)系的范圍是由DN大。本文用三章的篇幅按順序分別加以闡述。第六章闡述的是稻盛和夫經(jīng)營(yíng)哲學(xué)的文化淵源。中日兩國(guó)的文化關(guān)系一直是學(xué)界關(guān)注的重點(diǎn)。雖有學(xué)者提出稻盛哲學(xué)是中國(guó)儒家文化影響的產(chǎn)物,但筆者通過(guò)對(duì)稻盛的著作的文本分析,特別是對(duì)他引用中國(guó)典籍的情況的分析,得出結(jié)論他只是間接地局部地受到了中國(guó)文化影響。最后一章用文化傳播的理論分析了民間企業(yè)家組織盛和塾的活動(dòng)方式與發(fā)展歷程。盛和塾是一個(gè)由中小企業(yè)的年輕經(jīng)營(yíng)者組成的社會(huì)組織,其成立的目的原本只是經(jīng)驗(yàn)交流,但其成立二十余年來(lái),卻無(wú)形中擔(dān)負(fù)起傳播稻盛和夫的思想理念的任務(wù)。稻盛和夫的文化行為和企業(yè)家精神透過(guò)盛和塾,對(duì)同本社會(huì)的特定階層產(chǎn)生了潛移默化的影響。盛和塾在美國(guó)成立“分塾”,更屬于文化交流史上帶有標(biāo)志性的事件。參考文獻(xiàn)部分則列出本文征引的中、日文獻(xiàn)論著。關(guān)鍵詞經(jīng)營(yíng)哲學(xué);企業(yè)實(shí)踐;稻盛和夫;中日比較文化IL
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簡(jiǎn)介:國(guó)內(nèi)圖書分類號(hào)B00國(guó)際圖書分類號(hào)100哲學(xué)碩士學(xué)位論文企業(yè)社會(huì)責(zé)任的管理哲學(xué)研究碩士研究生導(dǎo)師申請(qǐng)學(xué)位級(jí)別學(xué)科、專業(yè)所在單位授予學(xué)位單位魯樹明李景春教授哲學(xué)碩士馬克思主義哲學(xué)文法學(xué)院燕山大學(xué)燕山大學(xué)碩士學(xué)位論文原創(chuàng)性聲明本人鄭重聲明此處所提交的碩士學(xué)位論文企業(yè)社會(huì)責(zé)任的管理哲學(xué)研究,是本人在導(dǎo)師指導(dǎo)下,在燕山大學(xué)攻讀碩士學(xué)位期間獨(dú)立進(jìn)行研究工作所取得的成果。據(jù)本人所知,論文中除已注明部分外不包含他人已發(fā)表或撰寫過(guò)的研究成果。對(duì)本文的研究工作做出重要貢獻(xiàn)的個(gè)人和集體,均已在文中以明確方式注明。本聲明的法律結(jié)果將完全由本人承擔(dān)。作者簽字日期年月日燕山大學(xué)碩士學(xué)位論文使用授權(quán)書企業(yè)社會(huì)責(zé)任的管理哲學(xué)研究系本人在燕山大學(xué)攻讀碩士學(xué)位期間在導(dǎo)師指導(dǎo)下完成的碩士學(xué)位論文。本論文的研究成果歸燕山大學(xué)所有,本人如需發(fā)表將署名燕山大學(xué)為第一完成單位及相關(guān)人員。本人完全了解燕山大學(xué)關(guān)于保存、使用學(xué)位論文的規(guī)定,同意學(xué)校保留并向有關(guān)部門送交論文的復(fù)印件和電子版本,允許論文被查閱和借閱。本人授權(quán)燕山大學(xué),可以采用影印、縮印或其他復(fù)制手段保存論文,可以公布論文的全部或部分內(nèi)容。保密□,在年解密后適用本授權(quán)書。本學(xué)位論文屬于不保密□。請(qǐng)?jiān)谝陨舷鄳?yīng)方框內(nèi)打“√”作者簽名日期年月日導(dǎo)師簽名日期年月日
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簡(jiǎn)介:分類號(hào)S852學(xué)校代碼10129UDC570833學(xué)號(hào)2009201047非洲豬瘟病毒VP73蛋白的原核表達(dá)純化及單克隆抗體的制備與鑒定PROKARYOTICEXPRESSIONANDPURIFICATIONOFPROTEINVP73INAFRICANSWINEFEVERVIRUS,ANDPREPARATIONANDIDENTIFICATIONOFMONOCLONALANTIBODIES申請(qǐng)人陳軼學(xué)科門類農(nóng)學(xué)學(xué)科專業(yè)基礎(chǔ)獸醫(yī)學(xué)研究方向獸醫(yī)藥理學(xué)與毒理學(xué)指導(dǎo)教師曹金山教授吳曉東副研究員論文提交日期二〇一二年五月體類型為L(zhǎng)GGI亞類;其腹水效價(jià)達(dá)到1024105以上;該株單抗特異性針對(duì)目的蛋白而非針對(duì)組氨酸標(biāo)簽蛋白,且與其他病毒的蛋白無(wú)交叉反應(yīng),特異性較好。關(guān)鍵詞非洲豬瘟病毒;VP73蛋白;原核表達(dá);單克隆抗體;
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簡(jiǎn)介:分類號(hào)華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文密級(jí)非洲豬瘟病毒間接ELISA抗體檢測(cè)方法的建立及其桿狀病毒載體疫苗的構(gòu)建ESTABLISHMENTOFANINDIRECTELISAMETHODFORTHESERUMANTIBODIESDETECTIONANDCONSTRUCTIONOFRECOMBINANT1一■●?●●●●●“●1一TT●BACUL0VLRUSVACCLNEOIAIRLCANSWLNE■。EVERVLRNS研究生學(xué)號(hào)指導(dǎo)教師指導(dǎo)小組靳雯雯2011302110156曹勝波教授陳煥春院士傅振芳教授宋云峰副教授專業(yè)預(yù)防獸醫(yī)學(xué)獲得學(xué)位名稱農(nóng)學(xué)碩士研究方向動(dòng)物傳染病獲得學(xué)位時(shí)間2014年6月華中農(nóng)業(yè)大學(xué)動(dòng)科動(dòng)醫(yī)學(xué)院二。一四年六月非洲豬瘟病毒間接ELISA抗體檢測(cè)方法的建立及其桿狀病毒載體疫苗的構(gòu)建目錄摘要????????????????????????????????????????????IABSTRACT...............................................................................................................................III英文縮略語(yǔ)表ABBREVIATION??????????????????????V1.文獻(xiàn)綜述??????????????????????????????11.1非洲豬瘟的歷史及流行病學(xué)????????????????????11.1.1非洲豬瘟的歷史??????????????????????.11.1.2非洲豬瘟的流行病學(xué)????????????????????.21.2非洲豬瘟的病原學(xué)特性??????????????????????31.2.1病毒的分類、形態(tài)與結(jié)構(gòu)??????????????????.31.2.2基因組的結(jié)構(gòu)與功能????????????????????.31.2.3非洲豬瘟病毒的理化特性??????????????????..41.3非洲豬瘟的臨床癥狀和病理變化??????????????????41.3.1非洲豬瘟的臨床癥狀????????????????????.41.3.2非洲豬瘟的病理變化????????????????????.51.4非洲豬瘟實(shí)驗(yàn)室診斷技術(shù)?????????????????????51.4.1病原學(xué)診斷技術(shù)??????????????????????.51.4.2血清學(xué)診斷技術(shù)??????????????????????.71.5非洲豬瘟疫苗研究進(jìn)展??????????????????????81.6桿狀病毒表達(dá)載體系統(tǒng)研究進(jìn)展??????????????????91.6.1桿狀病毒表達(dá)載體系統(tǒng)簡(jiǎn)介?????????????????.91.6.2桿狀病毒介導(dǎo)外源基因在哺乳動(dòng)物細(xì)胞中的表達(dá)????????101.6.3桿狀病毒載體疫苗的應(yīng)用??????????????????112.研究目的與意義??????????????????????????..133.實(shí)驗(yàn)材料?????????????????????????????..153.1細(xì)胞、菌種、毒株、質(zhì)粒與實(shí)驗(yàn)動(dòng)物???????????????.153.2血清樣品???????????????????????????.153.3本研究中所用的寡核苷酸引物??????????????????.153.4培養(yǎng)基、緩沖液的配制?????????????????????.153.5主要試驗(yàn)材料及儀器??????????????????????.174.實(shí)驗(yàn)方法?????????????????????????????..19
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簡(jiǎn)介:電子科技大學(xué)碩士學(xué)位論文小麥非洲黑麥雙二倍體在異源多倍體化過(guò)程中的變異與分子標(biāo)記研究姓名曾雪申請(qǐng)學(xué)位級(jí)別碩士專業(yè)生物物理學(xué)指導(dǎo)教師楊足君20090523摘要甲基化位點(diǎn)的絕對(duì)數(shù)量以及相對(duì)數(shù)量都有不同程度的改變。這些變異和差異有可能在一定程度上與雙二倍體和親本之間的基因差異表達(dá)有關(guān),進(jìn)而有可能與雙二倍體進(jìn)化有關(guān)。關(guān)鍵詞非洲黑麥ISSR;遺傳多態(tài)性;特異PCR分子標(biāo)記;DNA甲基化IL
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簡(jiǎn)介:河南農(nóng)業(yè)大學(xué)碩士學(xué)位論文新型光源下(LED、CCFL)非洲菊組織培養(yǎng)姓名李高燕申請(qǐng)學(xué)位級(jí)別碩士專業(yè)園林植物與觀賞園藝指導(dǎo)教師何松林20090601河南農(nóng)業(yè)大學(xué)2009屆碩士學(xué)位論文英文摘要STUDIES0NAPPLICATIONOFNOVELRADIATIONLEDANDCCFLRESOURCEININVITROGERBERAJAMESONIISUPERVISERPROFHESONGLINMASTERCANDIDATELIGAOYANABSTRACTLIGHTSOURCEISONEOFTHEMOSTIMPORTANTFACTORFORTISSUECULTURE,ITISNOTONLYPROVIDERADIANTENERGYFORPLANTPHOTOSYNTHESIS,BUTALSOPROVIDESIGNALSFORTHEPLANTTOADJUSTITSDEVELOPMENTTHEPURPOSEOFTHISEXPERIMENTISTOGETHIGHQUALITYINVITROANDLARGESCALEPRODUCTIONTHEUSEOFFIXEDWAVELENGTH,LIGHTINTENSITYADJUSTABLELIGHTEMITTINGDIODESANDTHECOLDCATHODEFLUORESCENTLAMPASALIGHTSOURCEUSEGERBERA’’RUIKOUFOR’’THEEXPERIMENTPLANTMATERIALTOEXPLORETHEEFFECTIVENESSOFLIGHTEMITTINGDIODESANDTHECOLDCATHODEFLUORESCENTLAMPINTHEPRODUCTIONFORGERBERAJAMESONIIINVITRO,ANDALSOCOMPARISONWITHORDINARYFLUORESCENTLAMPTOGERBERAFACTORYFORCOSTING1APPLICATIONOFLIGHTEMITTINGDIODELEDASLIGHTSOURCEFORINVITROCULTUREOFGERBERAJAMESONIITHERESULTSSHOWEDTHATLIGHTEMITTINGDIODELIGHTSOURCEMAKEABETTERROLEINPROMOTINGTHEGROWTHOFGERBERAJAMESONII砌VITROANDTHEGROWTHOFTRANSPLANTEDTHANCONVENTIONALFLUORESCENTLAMPGERBERAVARIETIES“RUIKOU’’INTHEEXPERIMENT,INTHE50I,TMOLM。2S~UNDERLIGHTINTENSITYOFTHERED/BLUERATIOOF55UNDERCONDITIONSOFLIGHTQUALITYGROWTHBEST,PLANTHEIGHT,LEAFNUMBER,LEAFLENGTH,LEAFSPANREACHED572MM,81,181MM,148LTLNL,F(xiàn)RESHWEIGHTFORTHEWHOLE6414MG,DRYWEIGHTFORTHEWHOLE643MG2APPLICATIONSCOLDCATHODEFLUORESCENTLAMPCCFLASALIGHTSOURCEFORINVITROCULTUREOFGERBERAJAMESONIITHERESULTSSHOWEDTHATCOLDCATHODEFLUORESCENTLAMPMAKEABETTERROLEINPROMOTINGTHEGROWTHOFGERBERAJAMESONIIINVITROANDTHEGROWTHOFTRANSPLANTEDTHANCONVENTIONALFLUORESCENTLAMPGERBERAVARIETIES‘RUIKOU’INTHEEXPERIMENTINTHE30PMOLM’2S。1UNDERLIGHTINTENSITYOFTHERED/BLUERATIOOF73,UNDERCONDITIONSOFLIGHTQUALITYGROWTHBEST,PLANTHEIGHT,LEAFNUMBERLEAFLENGTH,LEAFSPAN,ROOTLENGTHREACHED485MLTL,80,160MM,150MM,291MM,F(xiàn)RESHWEIGHTFORTHEWHOLE6414MG,DRYWEIGHTFORTHEWHOLE643MG3THEUSEOFLEDANDCCFLLIGHTSOURCE,TISSUECULTUREROOMSDECREASEDPOWERCONSUMPTIONACCFLLIGHTSOURCEASANEXAMPLE,THEPRODUCTIONOFONEMILLIONTESTTUBESEEDLINGSGERBERAPOWERCONSUMPTIONCOSTOFO105KWH/BOTTLEOF5/BOTTLE,THETOTALANNUALELECTRICITYCONSUMPTIONFORTHE264432KWH,ELECTRICITYCOSTS264432YUAN1YUANININDUSTRIALELECTRICITY/KWHBASIS,ITCANREDUCECOSTSEACHYEARNEARLY60,000YUANTHANTHEUSEOFFLUORESCENTLAMPS42
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簡(jiǎn)介:河南農(nóng)業(yè)大學(xué)碩士學(xué)位論文非洲鴕鳥消化管解剖學(xué)和組織學(xué)觀察及其功能的初步研究姓名喻道友申請(qǐng)學(xué)位級(jí)別碩士專業(yè)基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師梁宏德閆若潛20091001W南農(nóng)業(yè)人學(xué)09扁獸仄壩L論義英義摘要PRELIMINARYSTUDYONTHEANATOMYANDHISTOLOGICALEXAMINATIONANDTHEFUNCTIONOFTHEDIGESTIVETRACTOFAFRICANOSTRICHSUPERVISORPROF.LIANGHONGDEMASTERCANDIDATEYUDAOYOUABSTRACTAFRICAOSTRICHBELONGTOAYES,RATITAE,STRUTHIONIFORME,STRUTHIONIDAE,STMTHIO,STRUTHIOCAMELUSANDARETHELARGESTANDSTRONGESTBIRDSINTHEWORLD.THEVGROWFAST,RESISTANTTOCRUDEFEED,ANDISAKINDOFLARGEHERBIVOROUSBIRDS.WITHTHEINCREASINGAWARENESSOFTHEAFRICALLOSTRICH,THESIZEOFTHEIRCULTUREHAVEGRADUALLYEXPANDED.ITISVITALTHATTHEDISEASECONTROLANDPREVENTIONFORTHEOSTRICHTOIREPROVETHEIRPERFORMANCE.INSTRENGTHENINGTHEDISEASECONTROLANDPREVENTIONOFTHEAFRICANOSTRICHTOIMPROVETHEPERFORMANCEOFTHEIRPRODUCTION,ATTHESAMETIME,TOSTUDYITSDIGESTIVETRACTMORPHOLOGYANDHISTOLOGICALFEATURES,TOPROVIDESOMEMORPHOLOGICALEVIDENCEFORTHEBEHAVIOR’PHYSIOLOGY,PATHOLOGY,ECOLOGY,ASWELLASARTIFICIALFEEDINGANDREPRODUCTIONOFTHEAFRICANOSTRICH.ANDTHENPROVIDETHEVALUABLETHEORETICALBASISFORTHELARGESCALEBREEDINGANDDISEASECONTROLANDPREVENTIONOFAFRICANOSTRICHES.ATFIRSTWEOBSERVETHEESOPHAGUS,STOMACH。SMALLINTESTINEANDLARGEINTESTINEAFRICANOSTRICHTHROUGHTHEEYESANDTHEGENERALANATOMICALOBSERVATIONINTHISEXPERIMENT.AIMINGTOUNDERSTANDTHEDIGESTIVELOCATIONANDSTRUCTURALFEATURESOFTHEAFRICANOSTRICH.THEESOPHAGUSISLONGER,STARTINGINTHERETROPHARYNGEALANDTHENEXTENDTOTHECARDIAINTHEGLANDULARSTOMACH.ANDTRACHEAACCOMPANIED.STOMACHISCONSISTEDOFWELLDEVELOPEDGLANDULARSTOMACHANDMUSCULARSTOMACHCONSISTS,ANDFRONT.ENDISTHEGLANDULARSTOMACH,THEBACK.ENDISTHESTOMACHMUSCLES.MUSCULARSTOMACHISDIRECTLYLINKEDWITHTHEGLANDULARSTOMACH.ANDISLOCATEDINTHE1EFTSIDEOFTHEGLANDULARSTOMACH.THESMALLINTESTINEISOBVIOUSLYDIVIDEDINTODUODENUM.JEJUNUMANDILEUM,DUODENUMFROMTHEPYLORUSFROMTHEMUSCULARSTOMACH.ANDTHENTOTHERIGHTSIDEOFABDOMENTOFORMADUODENALTWISTINGORBENDINTO”U”.SHAPEDLOOPOFINTESTINE.WHENTOTHEFIGHTKIDNEYTUMBACKTOTHEFRONTOFTHEREAR,BEHINDTHEGLANDULARSTOMACHISIEJUNUM.ILEUMISLOCATEDATTHELOWERRIGHTCOLLIEROFTHEDUODENUM.ANDLINKWITHTHEBETHSIDESOFTHECECUMASSOCIATEDBYILEOCECALLIGAMENT.THELARGEINTESTINEINCLUDSTHETWOCAECUMS,COLONANDRECTUM,THECAECUMSTARTSFROMTHEBETHSIDESOFTHEWELDBETWEENILEUMANDCOLON.ILEOCECALLIGAMENTATTACHEDTOBOTHSIDESOFILEUM.COLONISLONGERANDSUSPENDFROMTHEMESENTERICNODEOFTHEBOTTOMOFTHELUMBARSPINE.RECTUMSUSPENDINTHEDORSALOFABDOMINALCAVITYBYASHORTDORSAIMESANGIAL.THEHISTOLOGICALSTRUCTURESOFDIGESTIVESYSTEM、WRESTUDIEDBYPARA硪NSECTIONINGANDH.ESTAINING.ITISINDICATEDTHATAFRICANOSTRICH’SDIGESTIVETRACTISCOMPOSEDOFFOURCONVENTIONALLAYERS.111EGROSSUSPLICA,DEVELOPEDMUSCULARIS,AND27
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簡(jiǎn)介:揚(yáng)州大學(xué)碩士學(xué)位論文非洲豬瘟病毒和古典豬瘟病毒雙重PCR及雙重?zé)晒釶CR檢測(cè)方法的建立與應(yīng)用姓名張倩申請(qǐng)學(xué)位級(jí)別碩士專業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師王志亮劉秀梵20100401Ⅱ揚(yáng)州大學(xué)碩士論文端和3’端分別標(biāo)記了FAY熒光素和TAMRA熒光素,在檢測(cè)CSFV的探針5’端和3’端分別標(biāo)記了VIC熒光素和TAMRA熒光素。由于ASFV是DNA病毒而CSFV是RNA病毒,所以先構(gòu)建了一個(gè)含有目的片段的CSFV的陽(yáng)性質(zhì)粒,這個(gè)陽(yáng)性質(zhì)粒也包含著熒光PCR反應(yīng)針對(duì)的序列,所以既可以作為PCR反應(yīng)的模板也可以作為熒光PCR反應(yīng)的模板。通過(guò)對(duì)雙重PCR引物濃度、退火溫度等反應(yīng)條件的優(yōu)化,篩選出最佳反應(yīng)條件,建立雙重PCR檢測(cè)方法。對(duì)于雙重?zé)晒釶CR,主要對(duì)引物和探針的濃度比進(jìn)行優(yōu)化,通過(guò)矩陣法篩選出最佳引物與探針的濃度比,建立雙重?zé)晒釶CR檢測(cè)方法,并對(duì)這兩種方法的特異性、敏感性和重復(fù)性進(jìn)行了測(cè)定。結(jié)果表明只有特異性引物與其特異性模板才能擴(kuò)增出目的片段,用此法檢測(cè)藍(lán)耳病病毒PRRSV,乙腦病毒JEV,圓環(huán)病毒PCV,細(xì)小病毒PPV偽狂犬病毒PRV結(jié)果均為陰性,說(shuō)明這兩種方法都具有良好的特異性。對(duì)梯度稀釋的陽(yáng)性質(zhì)粒的檢測(cè)可知所建立的熒光PCR方法對(duì)ASFV最低可檢測(cè)出10個(gè)拷貝,CSFV最低可檢出100個(gè)拷貝,普通PCR只能檢測(cè)到NG級(jí)別,說(shuō)明熒光PCR方法敏感性比普通PCR方法至少高10倍。對(duì)從山東膠東地區(qū)豬場(chǎng)采集的156份豬的血樣和豬病料用雙重PCR進(jìn)行檢測(cè)未發(fā)現(xiàn)有ASFV,有28份CSFV為陽(yáng)性,陽(yáng)性率為179%,這與單一PCR檢測(cè)的結(jié)果相符合,用雙重?zé)晒釶CR對(duì)相同的樣本進(jìn)行檢測(cè),也沒(méi)有發(fā)現(xiàn)ASFV,有30份CSFV為陽(yáng)性,陽(yáng)性率為192%,對(duì)PCR檢出為陰性而熒光PCR檢出為陽(yáng)性的病料進(jìn)行重復(fù)檢測(cè)并測(cè)序。關(guān)鍵詞非洲豬瘟病毒;豬瘟病毒雙重PCR;雙重?zé)晒釶CR;VP72基因;5’UTR
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簡(jiǎn)介:內(nèi)蒙古師范大學(xué)碩士學(xué)位論文蒙古族游牧文化的生態(tài)哲學(xué)審視姓名王全申請(qǐng)學(xué)位級(jí)別碩士專業(yè)科學(xué)技術(shù)哲學(xué)指導(dǎo)教師包和平20100617ABSTRACTSTARTINGFROMTHE20THCENTURY,THECHINESEENVIRONMENTALISSUES,ESPECIALLYINNORTHERNINNERMONGOLIAGRASSLANDECOLOGICALENVIRONMENT.CAUSEDWIDESPREADCONCERNINVARIOUSCIRCLESATHOMEANDABROAD.FACEDTHEENDANGEREDBROKENSITUATIONOFINNERMONGOLIAGRASSLANDECOSYSTEM,MANYDOMESTICANDFOREIGNSCHOLARS,ARESEEKINGTHECAUSESOFTHISSITUATIONWAYOUTFROMDIFFERENTFIELDSSUCHASTHEINSTITUTION,ECONOMYANDCULTURE.THEARTICLEANALYSEDTHEMONGOLIANNOMADICCULTUREANDTHEIRROOTCAUSESOFTHECHANGESFROMTHEVIEWOFECOLOGICALHILOSOPHY.MONGOLIANNOMADICCULTUREISSOCALLEDTHATNOMADSINTHEMONGOLIANPLATEAUREGIONFORTHOUSANDSOFNOMADICLIFEOFTHEPROCESSOFPRODUCTION,ACCUMULATIONOFHOWTOCOORDINATEPEOPLEANDLIVESTOCK,LIVESTOCKANDPASTURE,F(xiàn)ORAGEANDPASTURECONFLICTSBETWEEN,ANDNOMADICPRACTICE,GRADUALLYCREATINGCOMPLETESETSOFTHEINNERMONGOLIAPLATEAUUNIQUENATURALCONDITIONSOFPRODUCTIONANDLIFESTYLE;SOCIALSYSTEMS,CUSTOMS,ANDPHILOSOPH3,ANDRELIGION.MONGOLIANNOMADICCULTUREISAKINDOFECOLOGICALCULTURE,WHICHCONTAINSASIMPLEECOLOGICALONTOLOGY,EPISTEMOLOGYANDECOLOGICALVALUESOFECOLOGICALTHINKING.THESEPLAINGRASSLANDECOLOGICALPHILOSOPHYOFTODAYHASAPOSITIVEROLEINRESTORATIONOFTHEECOLOGICALENVIRONMENT.FROMTHELATEQINGDYNASTY,ALARGENUMBEROFIMMIGRANTS,PARTICULARLYAFTERTHEFOUNDINGOFTHERAPIDINCREASEINPOPULATIONLEDTOAGROWINGPOPULATIONOFINNERMONGOLIAGRASSLAND;WITHUNRESTRICTEDCULTIVATIONTOMOSTOFTHEGRASSLANDAWAY;THEREBYTHATUNDERMINEDTHETHE¨GRASS。ANIMAL。HUMAN”INTERDEPENDENTFOODCHAINOFINNERMONGOLIAGRASSLANDECOSYSTEMANDSOTHATTHEGRASSLANDDEGRADATEDANDDESERTIFICATED.THERESULTISTHATTHENOMADICWAYOFPRODUCTIONDECLINEANDTHEMONGOLIANNOMADICCULTUREFACESEXTINCTION.BUTTHISCANNOTBETHEROOTCAUSESOFCHANGEOFMONGOLIAN
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上傳時(shí)間:2024-03-03
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