簡介：復(fù)旦大學(xué)碩士學(xué)位論文黃瓜鈍綏螨AMBLYSEIUSCUCUMERIS生物學(xué)與生態(tài)學(xué)若干問題的研究姓名李新義申請(qǐng)學(xué)位級(jí)別碩士專業(yè)動(dòng)物學(xué)指導(dǎo)教師楊琰云吳千紅200051●堡竺塑蘭竺竺皂苧查竺蘭竺壁蘭奠蘭BIOLOGICALSTUDIESINCLUDINGLABORATORYPOPULATIONLIFEPARAMETERSOFTHEPREDATORYMITEAMBLYSEIUSCUCUMERISACARINAPHYTOSEIIDAEABSTRACTTHEVARIOUSBIOLOGICALASPECTSANDLIFEHISTORYPARAMETERSWERESTUDIEDSYSTEMATICALLYBYPRESENTPAPER1THEBIOLOGICALTRAITSINTHELIFESPANWASOBSERVEDANDDESCRIBED2THEEFFECTOFDEPRIVATIONFOODOFACUCUMERISONTHEPREDATORYCAPABILITYWASSTUDIEDTHETIMEOFDEPRIVATIONOFFOODHASDEFINITELYEFFECTONTHEPREDATIONBEHAVIORACERTAINTIMEDERIVATIONOFFOODHASEF琵CTINACERTAINPERIODTHEPREDATORYCAPABILITYOFMATEDADULTFEMALEACUCUMERISWASDEVELOPEDWITHTHEAGEINCREASINGTILLREACHTHEMOSTSTRONGEST3THESERIESFUNCTIONATRESPONSESOFVARIOUSSTAGESOFACUCUMENSONTHEEGGSOF7KJROPHAGUSPUTRESCENTIAESCHRANKANDONTHEADULTFEMALEOFPOLYPHAGOTARSONEMUSLATUSBANKWERESTUDIED，ANDTHEHOILINGDISCEQUATIONWASAPPLIEDA11THEFUNOTIONALRESPONSESAREBELONGTOTYPEIIAMONGTHEPRTONYMPH，DEUTONYMPHANDMATEDADULTFEMALE。THEADULTMATEDFEMALEISTHEMOSTEFFICIENTPREDATORYSTAGE，ANDTHEATTACKRATE嘲ISTHEHIGHESTANDTHEHANDLINGTIME錨ISTHESHORTESTONBOTHEGGSOFZPUTRESCENFFAEANDMATEDADULTFEMALEP1ATUSTHEDEUTONYMPHRANKSTHESECONDEFFICIENTPREDATORYSTAGETHEEFFICIENCYOFPREDATIONOFACLLCTLMER／SONTHEPATUSMAKESTHESPECIESAPOTENTBIOLOGICALCONTROLAGENTTOCONTROLTHEPATUSINGREENHOUSE4THEDEVELOPMENTALTHRESHOLDTEMPERATUREANDACCLIMATIZATIONWEREPRESENTEDACCORDINGTOTHEDEVELOPMENTTIMEATVARIOUSTEMPERATURESTHEDEVELOPMENTALTIMEISDECREASINGASTHETEMPERATUREINCREASING，ANDHASTHEHYPERBOLICRELATIONWITHTHEVARIETYOFTEMPERATURE5FEDONZPUTRESCENTIAEATVARIOUSTEMPERATURESNETREDRODUCTIONRATER，INTFINSICRATEOFNATURALINCREASERM，F(xiàn)INITERATEOFINCREASE，DAYSFORPOPULATIONTODOUBLET，MEANGENERATIONTIME丁，LONGEVITY，TOTALFECUNDITY，F(xiàn)ECUNDITYRATEANDEGGEGGDEVELOPMENTALTIMEOFTHEPOPULATIONOFAC擬CLCMERISA11HAVEAPARABOLARELATIONWITHTEMPERATUREWHILETHETIMETO50％MORTALITYANDDEVELOPMENTALTIMEHAVEHYPERBOLICRELATIONZPUTRESCENTIAEISMORESUITABLETHENSPIDERMITEASFOODTOPROLIFERATEACUCULYLERIS25℃ISTHEMOSTFAVORABLEAMONGA11TEMPERATURES，RMIS02051WHICHISHIGHERTHANPAPERSANDTHESALTIEPARAMETERPRESENTEDBYSPIDERMITEASFOODKEYWORDSBIOLOGICALCONTROLBIOCONTR01，AR曲LYSEIUSCⅡC“，NES，F(xiàn)UNCTIONALRESPONSES，LIFETABLE2

簡介：東北農(nóng)業(yè)大學(xué)博士學(xué)位論文豬囊尾蚴的發(fā)育生物學(xué)及藥物作用機(jī)理姓名劉永杰申請(qǐng)學(xué)位級(jí)別博士專業(yè)基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師李慶章200051劉水杰英文摘要2000年5月DEVELOPMENTALBIOLOGYOFCYSTICERCUSCELLULOSAEAND枷CHANISMOFACTIONOFDRUGSABSTRACTTSOLIUMCYSTICERCOSISISANIMPORTANTPARASITICZOONOSISENDANGERINGTHEDEVELOPMENTOFHUSBANDRYANDHEALTHYOFHUMANINORDERTOSELECTANEFFECTIVEMEDICINEAGAINSTCYSTICERCOSISANDTOPROVIDEATHEORETICABASISFORCHEMOTHERAPY，THEAUTHORSTUDIEDFIRSTLYANDSYSTEMATICALLYDEVELOPMENTOFCYSTLCERCIINVIVOANDINVITROANDTHEEFFECTOFDRUGSONCYSTICERCIINDLFFERENTDEVELOPMENTALSTAGESBYOPTICAMICROSCOPEANDELECTRONICMICROSCOPETHEMORPHOLOGYANDSTRUCTUREOFCYSTIEERCIWEREOBSERVEDINDETAILHTECHNIQUE，INSITUEND一1ABELLING，WASAPPLIEDTODETECTQUALITATIVELYANDQUANTITATIVELYTHEAPOPTOTLCEELLPOSITIVEINDEXAIINCYSTICERCIANDHOSTSINCOMBINATIONWITHTHEDETECTIONOFTHE1EVELSOFNITRICOXIDENOANDAIOFLYMPHOCYTELAI，THECLINALGENESISOFCYSTICERCOSISANDPOSSIBLEMECHANISMOFACTIONOFTHEDRUGSAGAINSTCYSTICERCIWEREANALYZEDTHERESULTSWERESHOWEDASF0110WS1ANINVITROCULTURESYSTEM1NCORPORATINGACELLMONOLAYERWITHOUTGASBHASEWASFIRSTLYUSEDTOINDUCEMOSTOFTHEONCOSPHERESOFTSOLIUMTOENTERDOSTONCOSPERALDEVELOPMENTTHE1ARVALCANSURVIVEFOR16DAYS2INVITROHATCHEDONCOSPHERESWEREINJECTEDINTRAVENOUSLYINTOTHEMICETHEMJCEWEREAUTOPSIED2MONTHSAFTERINOCUFATIONCYSTICERCIWEREFOUNDINTHE1UNGAND56PERCENTMICEWEREINFECTEDTHEMICEINFECTEDINTRAVENOUSLYBYONCOSPHERESAREVALUABLEEXPERIMENTALANIMAMODELSFORSTUDYINGTS01IUMCYSTLCERCOSIS3CHANGESOFMORPH0109Y，HIST0109ICALSTRUCTUREANDULTRASTRUCTUREOFCCEL1ULOSAEINTHEDEVELOPMENTWEREFIRSTIYANDSYSTEMATICALLYSTUDIEDTHESIZEOFCYSTICERCIINTHESAMEHOSTWEREVARIABETHEYOUNGESTCYSTICERCIOBSERVEDWEREI9DAYS01D，BUTSUCKERSANDHOOKSWERENOTFOUNDINSC01EXREGIONHISTOLOGYSHOWEDTHATALOTOFCELLSACCUMULATEDATONEOFTHEPOLESANDATTHEPROLLFERATIONREGIONTHETEGUMENTINVAGINATEDWITHTHEDURATIONOFINFECTION，SCOLEXBEGANTODIFFERENTIATEANDPOSSESSEDSUCKERSANDHOOKSDURINGTHEDEVELOPMENTTHEBLADDERWALLISATRANSITORYEMBRYOONTHEBASISOFWHICHTHESC01EXDEVELOPS60DAYS】ATER，MOSTOFCYSTICERCIWEREMATURETHEULTRASTRUCTURESHOWEDTHECOMPONENTSOFCYSTICERCIINCREASEDINNUMBERANDEXTENTBUTNONEWCELLSORTISSSUESAPPEAREDASTHECYSTICERCIGREWBLADDERWALLANDSOOLEXHADSOMEDIFFERENCESINULTRASTRUCTURESTHEBLADDERWALLSEEMSTOHAVEDIFFERENTFUNCTIONSFROMSCOLEXTHEBLADDERWALLMIGHTFUNCTIONINTHEABSORPTIONOFNUTRITIONWHILETHESCOLEXWASCONCERNEDWITHTHEFUTUREDEVELOPMENT2

簡介：近年來減毒細(xì)菌活載體因其能夠刺激機(jī)體產(chǎn)生針對(duì)活載體及其攜帶的外源抗原的體液、細(xì)胞免疫和局部粘膜免疫受到人們的廣泛關(guān)注特別是近期研究結(jié)果證明許多活細(xì)菌載體能夠作為DNA疫苗的運(yùn)送載體可以通過口服減毒細(xì)胞進(jìn)入機(jī)體從而誘導(dǎo)產(chǎn)生針對(duì)特定抗原的各種免疫應(yīng)答但迄今為止對(duì)活疫苗載體與宿主免疫系統(tǒng)間相互作用規(guī)律及其調(diào)控機(jī)制知之甚少這方面的免疫生物學(xué)研究對(duì)于研制新型基因工程疫苗將會(huì)提供重要的理論依據(jù)且對(duì)相關(guān)疾病的免疫防制有著重要的指導(dǎo)價(jià)值因此我們構(gòu)建了表達(dá)綠色熒光蛋白GFP的減毒鼠傷寒沙門氏菌闡明其在體內(nèi)、外感染試驗(yàn)的動(dòng)態(tài)分布及它與抗原呈遞細(xì)胞的相互作用規(guī)律這為沙門氏菌疫苗載體及相關(guān)基因工程疫苗的研究奠定了理論基礎(chǔ)1表達(dá)綠色熒光蛋白的重組減毒鼠傷寒沙門氏菌的構(gòu)建與鑒定2重組減毒鼠傷寒沙門氏菌感染動(dòng)力學(xué)3重組減毒鼠傷寒沙門氏菌與抗原呈遞細(xì)胞的相互作用綜上所述該研究構(gòu)建了表達(dá)綠色熒光蛋白的鼠傷寒沙門氏菌通過靜脈注射和口服途徑記錄了此細(xì)胞在體內(nèi)一些臟器的分布規(guī)律及誘導(dǎo)的抗體的變化結(jié)果顯示細(xì)菌數(shù)量及抗體效價(jià)變化均有一定的規(guī)律而且細(xì)菌數(shù)量與抗體效價(jià)之間有一定的相關(guān)性在體外用細(xì)胞系和腹腔巨噬細(xì)胞驗(yàn)證了此細(xì)菌具有感染細(xì)胞的能力在體內(nèi)感染實(shí)驗(yàn)中檢測(cè)了靜脈注射途徑肝臟、脾臟中巨噬細(xì)胞和樹突細(xì)胞的感染率同時(shí)檢測(cè)了口服后3天腹腔巨噬細(xì)胞的感染狀況結(jié)果顯示在體內(nèi)巨噬細(xì)胞和樹突細(xì)胞很快地?cái)z取了該重組菌這為起始保護(hù)性免疫反應(yīng)奠定了基礎(chǔ)

簡介：山東農(nóng)業(yè)大學(xué)博士后士學(xué)位論文蘋果角質(zhì)層的生物學(xué)和虎皮病的發(fā)生姓名張?jiān)暾?qǐng)學(xué)位級(jí)別博士后士專業(yè)果樹學(xué)指導(dǎo)教師束懷瑞2001121內(nèi)部結(jié)構(gòu)已經(jīng)被破壞。在表皮細(xì)胞及下皮細(xì)胞周圍也有有大量電子密致物，電子密致物進(jìn)入細(xì)胞時(shí)伴有小囊泡的形成，細(xì)胞內(nèi)部結(jié)構(gòu)包括質(zhì)體被破壞。貯藏至20周時(shí)，晚采果仍然沒有發(fā)生虎皮病，果皮細(xì)胞結(jié)構(gòu)完整，仍然可以見到質(zhì)體及線粒體。超微結(jié)構(gòu)中僅見表皮細(xì)胞上方有大量電子密致物，而表皮細(xì)胞下方只有少量電子密致物，且未或僅小部分接觸細(xì)胞。外層下皮細(xì)胞周圍未見有下移電子密致物，細(xì)胞內(nèi)部結(jié)構(gòu)完好，葉綠體結(jié)構(gòu)完整?？傊?，角質(zhì)層中的電子密致物在蘋果虎皮病發(fā)生過程中可向內(nèi)層表皮細(xì)胞、下皮細(xì)胞移動(dòng)，從外部破壞細(xì)胞，使細(xì)胞解體，產(chǎn)生褐變。推測(cè)角質(zhì)層中蠟層的消失及角質(zhì)層電子密致物的下移與小法尼烯的積累和氧化有關(guān)。研究表明在貯存過程中早采和晚采國光和紅星蘋果角質(zhì)層中的共軛三烯含量與虎皮病的發(fā)生存在不相關(guān)現(xiàn)象，進(jìn)一步證明了以共軛三烯含量來表示僅法尼烯氧化產(chǎn)物說明虎皮病的發(fā)生程度存在弊端。而RFC值與虎皮病的發(fā)生密切相關(guān)。RFC值可以表示角質(zhì)層中抗氧化水平。晚采和DPA處理的果實(shí)的抗氧化活性分別顯著高于早采果和未處理果，前者的RFC值也分別顯著高于后者。修正了A一法尼烯與共軛三烯的比值RFC值用于虎皮病預(yù)測(cè)的數(shù)學(xué)模型。模型可用于貯存過程中蘋集虎皮病的預(yù)測(cè)。通常在RFC值低于10時(shí)蘋果出庫至常溫時(shí)虎皮病即會(huì)發(fā)生弋關(guān)鍵詞蘋果；角質(zhì)層；蠟層；超微結(jié)構(gòu)；虎皮??；電子密致物；旺法尼烯；共軛三烯II

簡介：中國科學(xué)技術(shù)大學(xué)碩士學(xué)位論文同步輻射小麥引起的生物學(xué)效應(yīng)血源與基因工程Α干擾素抑制貯脂細(xì)胞增殖及膠原合成的比較研究姓名趙凌申請(qǐng)學(xué)位級(jí)別碩士專業(yè)細(xì)胞生物學(xué)指導(dǎo)教師顧月華200141中國科學(xué)技術(shù)人學(xué)預(yù)上學(xué)位論文第一部分同步輻射對(duì)小麥引起的生物學(xué)效應(yīng)摘要研究了不同劑量的同步輻射軟X射線源輻照小麥種子后產(chǎn)生的生物學(xué)效應(yīng)，以期從細(xì)胞和分子水平蛋白質(zhì)、酶及DNA等上揭示同步輻射軟X射線源對(duì)生物體的誘變效應(yīng)及其作用機(jī)制，為其應(yīng)用于輻射育種提供理論依據(jù)。I我們進(jìn)行了以下研究I檢測(cè)小麥幼苗細(xì)胞的脂質(zhì)過氧化作用及保護(hù)酶活性?？梢钥闯?，隨著同步輻射軟X射線劑量的增加，脂質(zhì)過氧化產(chǎn)物丙二醛MDA的含量也隨之增加；在保護(hù)酶系統(tǒng)中，過氧化氫酶CAT、過氧化物酶POD、超氧化物歧化酶SOD的活性變化不表現(xiàn)出同步性，表明三者之間存在相互協(xié)調(diào)的關(guān)系，處理組葉綠素含量及葉綠體中關(guān)鍵的保護(hù)酶抗壞血酸過氧化物酶APX的活性始終低于空白對(duì)照組。結(jié)果表明同步輻射軟X射線輻照對(duì)于小麥細(xì)胞保護(hù)酶活性及脂質(zhì)過氧化作用的影響很大。II采用單細(xì)胞凝膠電泳方法檢測(cè)了同步輻射軟X射線源對(duì)DNA造成的單鏈損傷，通過測(cè)定DNA遷移效應(yīng)，表明DNA損傷程度與輻照劑量呈顯著的正相關(guān)性。1ID采用TUNEL方法來進(jìn)行原位細(xì)胞凋亡檢測(cè)，并結(jié)合DAPI熒光染色，表明同步輻射軟X射線源輻照小麥種子可以使之產(chǎn)生不同程度上的細(xì)胞凋亡。通過統(tǒng)計(jì)細(xì)胞凋亡率，也表明損傷程度與輻照劑量成『F相關(guān)性。以上實(shí)驗(yàn)表明，作為一種新的誘變?cè)?，同步輻射軟X射線輻照麥類作物種子的生物學(xué)效應(yīng)顯著，它不僅能夠?qū)π←溂?xì)胞遺傳物質(zhì)造成損傷同時(shí)也能對(duì)相關(guān)的體內(nèi)保護(hù)酶系統(tǒng)產(chǎn)生調(diào)節(jié)作用，因此應(yīng)用同步輻射進(jìn)行農(nóng)業(yè)育種將有、極大的發(fā)展?jié)摿颓熬啊?關(guān)鍵詞同步輻射軟X射線源；小麥細(xì)胞；脂質(zhì)過氧化；保護(hù)酶系統(tǒng)；單細(xì)胞凝膠電泳；細(xì)胞凋亡

簡介：華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文湖北省玉米粗縮病病原分子生物學(xué)鑒定及玉米抗感病性研究姓名燕永亮申請(qǐng)學(xué)位級(jí)別碩士專業(yè)植物病理學(xué)指導(dǎo)教師侯明生于嘉林200251ABSTRACTMAIZEISONEOFTHEMAINCROPSINCHINA，ANDISALSOTHEIMPORTANTSUSCEPFIDEMATERIALFORFORAGE，LIGHTINDUSTRYANDMEDICINENOTONLYTHETOTALPLANTATIONAREABUTALSOTHEYIELDAREALLINTHETHIRDPLACE，ONLYBELOWTHATOFRICEANDWHEATINRECENTYEARS，WITHTHECHANGEOFTHECULTIVATEHABBITTHEPLANTATIONARCABECOMEBIGERANDBIGERANDTHESPECIESCHANGINGFIEQUENTLYOWINGTOTHEFARMERSIMPLYSEEKHIGHYIELDANDPLANTSENSITIVEMAIZESPEIEES，THUSCAUSEASERIESOFDISEASESBECOMEHIGHERTHANEVERWHILE，THEDISEASESNEVERHAPPENEORSELDOMHAPPENBEFOREONEOFTHESEEXAMPLESISTHEMAIZEROUGHDWARFDISEASEIT’SREPORTEDTHATTHEPATHOGENYCAUSEDTHEDISEASEISMRDVANDWHICHBELONGSTOTHEREOVIRIDAEFIJIVIRUSTHEVIRUSISSOSIMILAR誦也ANOTHER丘JIVIRUSRBSDVINBIOLOGIEALANDIMMANITICALCHARACTERTHATMANYSPECIALISTSREGARDTHESETWOVIRUSESASONETHROUGHANALYZINGTHENUCLEOTIDESSEQUENCEOFTHESEVIRUSES，WECANCLASSFYTHEMCORRECTLYONEISOLATEOFTHEPATHOGENYFROMHUBEIWASANALYZEDATTHEMOLECULARLEVELTHEGENOMICRNAELEETROPHORETICPROFILESSHOWEDTHEGENOMECOMPOSED10DSRNASEGMENTSSELECTTHES7，CLONETHEEDNABYRTPCRAMPLICATION，ANDTHENSEQUENCETHECLONEWHICHHASREGISTEDINTHEGENBANKANDTHENUMBERAF397874THERESULTOFTHESEQUENCEANALYSISDEMONSTRATEDTHATTHEGANOMEOFHUBEIISOLATEHASTHEHOMOLOGY、訪TLLMRDVIANDRBSDVJFOR853％AND937％ALSOTHETWOORFSINTHISSEQUENCEHASTHEHOMOLOGYWITHTHATOFMRDVIANDRBSDVJFORORFL，908％AND98％；ORF2，854％AND957％THERESNLTINDICATEDTHATTHEVIRUSWHICHCAUSETHISMAIZEDISEASEINHUBEIISRICEBLACKSTREAKEDROUGHVIRUSBASINGONTHESTUDYATMOLECULARLEVEL，WEALSOIDENTIFIEDTHERESISTENCEANDSENSITIVETOTHEDISEASEWITH64MAIZESPECIESINFIELDINTIANMENANDHANCHUANWHERETHEDISEASEWASVERYHEAVYFORSEVERALYEARSTHESEMAIZESPECIESWERECOLLECTEDFORMLIAONINGBEIJING，SICHUANANDSHANDONGPROVINCESTHERESULTSHOWSTHATTHEREARE21SPEIEESHAVE1LIGHRESISTENEETOTHEDISEASE，2SPEICESHAVEHIGHSENSITIVETOTHEDISEASE，THEOTHERSAREINTHEMIDDLEINASENSE，THERESULTISVERYIMPORTANTBECAUSEITMAYHELPTHEFARMERTOSELECTTHESUITABLESEEDKEYWORDSRICEBLACKSTREAKEDDWARFVIRUSMAIZEROUGHDWARFDISEASEEDNACLONINGSEQUENCEANALYSIS2

簡介：福建農(nóng)林大學(xué)碩士學(xué)位論文EUI基因?qū)﹄s交稻若干生物學(xué)特性和構(gòu)成產(chǎn)量因素的影響姓名章清杞申請(qǐng)學(xué)位級(jí)別碩士專業(yè)作物遺傳育種指導(dǎo)教師楊仁崔200251關(guān)鍵詞EUIL基因；EUI2基因；E．雜交稻；農(nóng)藝性狀；產(chǎn)量構(gòu)成；干物質(zhì)積累配合力

簡介：四川農(nóng)業(yè)大學(xué)碩士學(xué)位論文老鷹茶白粉菌生物學(xué)特性、侵染過程及抗性變化的初步研究姓名朱蓮申請(qǐng)學(xué)位級(jí)別碩士專業(yè)森林保護(hù)指導(dǎo)教師劉應(yīng)高20070601ABSTRACTPOWDERYMILDEWISADETRIMENTALDISEASEOFEAGLETEALITSEACOREANALEVLVARLANUGINOSEINTHECOMMERCIALGROWINGAREAINYAAN，SICHUANPROVINCETHISDISEASEINDUCESABNORMALLEAFSHAPESANDADVERSELYAFFECTSTHEEAGLETEAPRODUCTIVITYTHESTUDYRECORDEDANDDEMONSTRATEDTHESYMPTOMSANDSIGNSOFTHISDISEASEINDETAILS，ANDSYSTNNATICALLYSTUDIEDTHEPATHOGENICFIMGUS’SBIOLOGICALCHARACTERISTICSPATHOGENHOSTINTERACTIONS，ANDHOSTPLANTPHYSIOLOGICALANDBIOCHEMICALDEFENSERESPONSESACCORDINGTOTHEMORPHOLOGICALCHARACTERISTICSINCLUDINGTHESIZESOFTHECONIDIOPHOREANDCONDIUM，THEPATHOGENWASIDENTIFIEDASOIDIUMOIDIUMCALLGROWBOTHINTHEACIDORALKALINEENVIRONMENTTHESPOREGERMINATIONCANHAPPENEDINPHRANGES30120，ANDWITHTHEOPTIMUMPHFORSPORULATIONAT100THEOPTIMUMTEMPERATUREFORSPORULATIONRANGED15TO25“C，ANDTHEOPTIMUMHUMIDITYIS100％DARKORINDIRECTILLUMINATIONSAREADVANTAGEOUSTOSPOREFORMATIONTHEULTRAVIOLETLIGHTUVLISDEVASTATINGTOOIDIUMSPORE，ANDTHELONGERTHEUVLILLUMINATIONTHELESSTHEGERMINATIONTHISSTUDYSHOWEDTHATTHEREWERENOSPOREGERMINATIONAFTERTREATEDWITHUVLFOR30MININTHESTUDY。RESISTANCETOPOWDERYMILDEWCAUSEDBYPATHOGENICFUNGUSOIDIUMOF5COMMERCIALLYIMPORTANTEAGLETEACULTIVARSINSICHUANPROCINCEWEREEVALUMEDBOTHINTHEFIELDANDINTHELABORATORYONARTIFICIALLYINOCULATEDPLANTSRESULTSWERECONSISTENTFORTHETWOEVALUATIONMETHODS，ANDTHEREFORE，WECONCLUDEDTHATARTIFICIALLYINOCULATIONINTHELABORATORYISRELIABLEANDFEASIBLE，ESPECIALLYWHENALARGEAMOUNTOFCULTIVARSWEREINVOLVEDOUREVALUATIONSFOUNDTHAT，AMONGFIVEEAGLETEACULTIVARS，ONECULTIVARWASHIGHLYRESISTANT，TWOCULTIVARSWERESUSCEPTIBLE，ANDTHERESTTWOCULTIVARSWEREMEDIUMRESISTANTINTHESTUDY，ELECTRONICSCANNINGMICROSCOPEANDOPTICALMICROSCOPEWEREUSDTOOBSERVETHEHOSTPATHOGENHISTOLOGICALINTERACTIONSINDIFFERENTPHASESTHERESULTSSHOWEDTHATAT4HAFTERINOCULATION，THEPRIMARYGERMTUBESWEREEMERGEDFROMTHEGERMINATINGSPORESAT8HAFTERINOCULATIONTHEAPPRESSORIUMGERMTUBEFORMEDAPPRESSORIUMATL2HAFTERINOCULATIONAPPRESSORIUMFORMEDAPPRESSORIUMLOBEORSECONDAPPRESSORIUMLOBEANDAPPRESSOFIUMINDUCEHOSTCELLSFORMEDHALOAT24HAFERINOCULATIONAPPRESSORIUMDEVELOPEDFURTHERINTOHAUSTORIAAT48HAFTERINOCULATION，THEPRIMARYHYPHAEGREWFROMTHEAPEXOFTHEAPPRESSORIUMOVERTHESURFACEOFTHEHOSTEPIDERMALCELLSAT72HAFTERINOCULATIONPRIMARYHYPHAEDEVELOPEDINTOSECONDARYHYPHAETHETIMECONRSECHANGESINTHEACTIVITYOFPHOTOSYNTHESIS，PEROXIDASEPOD，CATALASELI

簡介：中國農(nóng)業(yè)大學(xué)碩士學(xué)位論文毒害艾美耳球蟲晚熟系選育及其生物學(xué)特性研究初報(bào)姓名董輝申請(qǐng)學(xué)位級(jí)別碩士專業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師索勛汪明200261中嗣農(nóng)業(yè)太學(xué)頸士學(xué)位論文EIMERIANECATRIXSELECTIONANDCHARACTERISTICSOFAPOSTCOCIOUSLINEABSTRACTASTRAINOFENECATRIXWASISOLATEDFROMSUSPENSIONSOFOOCYSTSBYTHEUSEOFTHESINGLEOOEYSTISOLATIONTECHNIQUETHEOOCYSTSOFTHESTRAINWEREELLIPTICA／INSHAPEANDHADAVERAGEDIMENSIONSOF19。50圭2。31X15。8L圭186NMITSPREPATENTPERIODWAS138HANDTHEPEAKOFOOEYSTPRODUCTIONOECUREDONTHE7THPOSTINFECTIONSTUDYOFITSPATHOGENECITYSHOWEDTHATTHETIMEANDDEGREEOFCLINICALSIGNSWEREDIFFERENTANDESS∞IATADWITHTHEDOSESINOCULATEDANDALLDOSESHADNEGATIVEEFFECTONTHEWEIGHTGAI娜OFTHEBIRDSANDCAUSEDDIFFERENTINTESTINALLESIONS一APOSTCOCIOUSLINEOFTHESTALINWASSELEETADBYREPEATEDPASSAGESOFOOCYSTSTHATWERELATELYCOLLECTEDFROMFAECESOFPREVIOUSLYINFECTEDCHICKENSCOMPAREDWITHTHEPARENTSTRAINTHEPOSTCOEIOUSLINEHADTHEFOLLOWINGCHARACTERISTICSTHEPEAKPRODUCTIONOFOOCYSTSWASTWODAYSLATER；THEPREPATENTPERK堪WASNOTPROLONGED，BUTTHEDIMENSIONOFSEVERALENDOGENOUSDEVELOPMENTSTAGESWASLARGERTHANTHATOFTHEEORRESPOND趣GS紐GESOFTHEPARENTSTRAIN；THEPATHOGENICITY鋪磕SSIMILARINCOMPARISONWITHTHEPARENTSTMIN；THEREPRODUCTIVECAPACITYANDTHEIMRAUNOGENICITY蝴HTGHRTHANAAATOFTLWPARENTSTRAINRESPECTIVELYKEYWORDSE矗NERTANECATRIX；POSTEOEIOUSLINE；SELECTION；CHARACTERISTICS2

簡介：中國農(nóng)業(yè)大學(xué)碩士學(xué)位論文綿羊胚胎期血漿促性腺激素生物學(xué)活性的變化和垂體細(xì)胞體外無血清培養(yǎng)模型的建立姓名曹興元申請(qǐng)學(xué)位級(jí)別碩士專業(yè)生理學(xué)指導(dǎo)教師崔勝200261里奎些查堂墮主絲苧90、120天和分娩時(shí)分別為10．190．27NG／ML、9．320．13NG／ML、7．320．21NG／ML、7．02／0．29N咖1?！危蠜把芯拷Y(jié)果提示『血漿B．FSH和B．LH在妊娠前期生物學(xué)濃度較骶，在妊娠中期生物學(xué)濃度達(dá)到最高，隨后生物學(xué)活性下降或保持恒定。并且雌雄胚胎BFSH和BLH之問存在著性別差異。與先前RIA方法對(duì)FSH和LH的研究相比，存在著較大的差異，說明促性腺激素生物學(xué)活性的測(cè)定方法比RJA方法更能準(zhǔn)確反應(yīng)胚胎期FSH和LH的生理功能。此外，本實(shí)驗(yàn)還建立了綿羊垂體細(xì)胞體外無血清培養(yǎng)模型，并且對(duì)培養(yǎng)不同時(shí)間的垂體細(xì)胞進(jìn)行了LHB的免疫細(xì)胞化學(xué)染色，以檢測(cè)培養(yǎng)過程中垂體細(xì)胞自主分泌LH的能力。結(jié)果在無血清培養(yǎng)4天后的垂體細(xì)胞，LHFL免疫陽性染色的細(xì)胞顯著性增加，說明此時(shí)垂體細(xì)胞具有較強(qiáng)的LH合成能力?！螇騊切紹論『2關(guān)鍵詞卵泡刺激素，促黃體生成素，綿羊胚胎，生物學(xué)活性，細(xì)胞培養(yǎng)，垂體，”1。。VX

簡介：浙江大學(xué)博士學(xué)位論文CPPU誘導(dǎo)瓠瓜單性結(jié)實(shí)生理和分子生物學(xué)機(jī)制研究姓名李英申請(qǐng)學(xué)位級(jí)別博士專業(yè)蔬菜學(xué)指導(dǎo)教師喻景權(quán)朱祝軍200241株光合作用增強(qiáng)，且CPPU處理株光合作用的增加更為顯著。這可能與CPPU處理后庫強(qiáng)的增強(qiáng)有關(guān)。4未授粉果和授粉果中的IAA和TZEATIN濃度在花后9D內(nèi)均呈上升趨勢(shì)，而在CPPU誘導(dǎo)的單性結(jié)實(shí)果中IAA濃度則呈明顯的下降趨勢(shì)，其濃度明顯低于未授粉果和授粉果實(shí)中的濃度，TZEATIN濃度則在花后3天達(dá)到最大值隨后快速下降。5NAA和GA3處理未經(jīng)授粉的瓠瓜子房不能顯著促進(jìn)其細(xì)胞分裂和膨大而不能產(chǎn)生單性結(jié)實(shí)果，而CPPU處理未經(jīng)授粉的瓠瓜子房則使其細(xì)胞數(shù)目和大小以及單果重都顯著高于未授粉和人工授粉果。6測(cè)定了瓠瓜果實(shí)組織中的兩條D一型周期蛋白同源基因的CDNA全長序列。經(jīng)序列的同源性及系統(tǒng)發(fā)生分析比較，它們均屬D3周期蛋白基因，且分別命名為LAGLE；CYCD3；1和LAGLE；CYCD3；2。其推測(cè)的氨基酸序列都含有一個(gè)RETINOBLASTOMABINDINGMOTIF和PESTDESTRUCTIONMOTIF。授粉受精后，LAGLE；CYCD3；12轉(zhuǎn)錄水平增加。CPPU處理也使其轉(zhuǎn)錄水平增加且作用效果更為迅速顯著。7測(cè)定了瓠瓜果實(shí)組織中的一條酸性轉(zhuǎn)化酶基因的CDNA全長序列，長2164BP，含1992BP的編碼框，編碼663個(gè)氨基酸。經(jīng)序列的同源性及系統(tǒng)發(fā)生分析比較，該酸性轉(zhuǎn)化酶基因?qū)儆谝号菪退嵝赞D(zhuǎn)化酶，并命名為LIAL。CPPU處理或授粉受精都促進(jìn)了酸性轉(zhuǎn)化酶的轉(zhuǎn)錄水平和酶活性。關(guān)鍵詞CPPU，瓠瓜，單性結(jié)實(shí)，座果率，果實(shí)發(fā)育，細(xì)胞分裂，內(nèi)源激素，源庫關(guān)系，光合作用，14C小乜物，C02加富，酸性轉(zhuǎn)化酶，C≯CD3

簡介：大連水產(chǎn)學(xué)院碩士學(xué)位論文海灣扇貝自交系生物學(xué)的初步研究姓名劉述錫申請(qǐng)學(xué)位級(jí)別碩士專業(yè)水產(chǎn)養(yǎng)殖指導(dǎo)教師張國范200261劉述錫碩士論文海灣扇貝自交系生物學(xué)的初步研究作用。5BSDL系列親本的殼長都分別高于其對(duì)應(yīng)自交子一代成貝的殼長均值，除了BSDLL4外，親本的鮮重都分別高于其對(duì)應(yīng)自交子一代成貝的鮮重均值。BSDL系列自交系成貝的殼長的標(biāo)準(zhǔn)差在322928范圍內(nèi)變化，鮮重標(biāo)準(zhǔn)差在235880范圍內(nèi)變化，較高的方差變化給定向選擇提供了條件。6BSDL系列自交系成貝越冬成活率最高為143％，最低為0，平均越冬成活率為53％，而對(duì)照系的越冬成活率僅為295％。表現(xiàn)了自交系在低溫海域的越冬優(yōu)勢(shì)。7BSDL07養(yǎng)成后發(fā)現(xiàn)其顏色性狀出現(xiàn)分離，成活的成貝共61個(gè)，其中20個(gè)紅褐色，41個(gè)黑色，比例近似為21。顏色性狀是一個(gè)較易觀察的性狀，如能穩(wěn)定的遺傳下去，可以建立不同的品系，本實(shí)驗(yàn)為今后的海灣扇貝的顏色品系的建立提供了一個(gè)鋪墊。、J／?！P(guān)鍵詞海灣扇貝7不同遺傳毒甄，自交系浮游幼蟲_生長，養(yǎng)成生長，越冬成活率

簡介：福建農(nóng)林大學(xué)碩士學(xué)位論文腐食酪螨TYROPHAGUSPUTRESCENTIAESCHRANK生物學(xué)及抗藥性機(jī)理研究姓名于曉申請(qǐng)學(xué)位級(jí)別碩士專業(yè)農(nóng)藥學(xué)指導(dǎo)教師范青海200251福建農(nóng)林大學(xué)碩士學(xué)位論文于曉腐食酪螨生物學(xué)及抗藥性杌理研究來特靈和樂斯本，其LC50值分別為3204793MGLL、1269697MGL。1090436MGL～。Ⅱ一和5磷化氫對(duì)腐食酪螨敏感種群的熏蒸毒力為284MIRI，室內(nèi)抗性選育至16代，抗性增加到475倍。抗性種群的LT50與選育代數(shù)的回歸曲線方程為Y35970211120XRO9881。6不同種群體內(nèi)蛋白質(zhì)含量差異顯著，并且蛋白質(zhì)含量隨抗性水平的提高而逐漸增加，抗性種群蛋白質(zhì)含量與其抗性倍數(shù)的回歸曲線方程為YO395300909XRO9515。7腐食酪螨抗磷化氫機(jī)理之一是體內(nèi)羧酸酯酶和乙酰膽堿酯酶的活性發(fā)生變化。羧酸酯酶和乙酰膽堿酯酶的活性隨抗性水平的提高而增強(qiáng)?？剐苑N群羧酸酯酶比活力、乙酰膽堿酯酶比活力與抗性倍數(shù)的回歸曲線方程分別為Y7152676757XR209515、YO039900635XR09714。文／關(guān)鍵詞；腐食酪螨I有效積溫；毒，歡敏感種群；抗性種群I抗性機(jī)理

簡介：浙江大學(xué)博士學(xué)位論文BT水稻對(duì)靶標(biāo)害蟲二化螟的生物學(xué)與病理學(xué)效應(yīng)姓名王世貴申請(qǐng)學(xué)位級(jí)別博士專業(yè)農(nóng)業(yè)昆蟲與害蟲防治指導(dǎo)教師胡萃200251外．隨取食時(shí)間延長，各類血細(xì)胞數(shù)量DHC及血細(xì)胞總數(shù)THC均呈遞減的趨勢(shì)。從各類血細(xì)胞所占血細(xì)胞總數(shù)的百分比來看，漿血胞在取食后比例下降，而顆粒血胞則比例增加，其余珠血胞、囊血胞的變化不明顯。5．BT水稻對(duì)二化螟腸道消化酶的影響。結(jié)果表明，二化螟幼蟲腸道消化液包括兩大類蛋白酶，即類胰蛋白酶和類胰凝乳蛋白酶。幼蟲取食BT水稻后弱堿性類胰蛋白酶和類胰凝乳蛋白酶的活性總體呈下降趨勢(shì)，而強(qiáng)堿性類胰蛋白酶在攝入BT水稻后活性則呈上升趨勢(shì)且高于對(duì)照組。6．BT水稻對(duì)二化螟幼蟲取食行為的影響。結(jié)果表明，幼蟲對(duì)對(duì)照的選擇系數(shù)顯著高于、甚至極顯著地高于BT水稻。而在兩種BT水稻品系間，幼蟲對(duì)克螟稻1號(hào)的選擇系數(shù)要高于克螟稻2號(hào)，與它們各自的抗蟲性水平是相一致的。二化螟3齡幼蟲在轉(zhuǎn)BT基因水稻上的鉆蛀次數(shù)、蛀入時(shí)間等方面都高于對(duì)照，而蛀入成功率則比對(duì)照為低，但差異都不顯著。在鉆蛀部位的選擇上，幼蟲都表現(xiàn)出喜好植株莖桿部位，其次是葉片主脈。關(guān)鍵詞BT水稻，二化螟，生長發(fā)育，致死效應(yīng)，亞致死效應(yīng)，組織病理，免疫系統(tǒng)，取食行為III

簡介：華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文珍珠貝的精子生物學(xué)及其在轉(zhuǎn)基因技術(shù)中的應(yīng)用姓名蔡云川申請(qǐng)學(xué)位級(jí)別碩士專業(yè)水產(chǎn)養(yǎng)殖指導(dǎo)教師龔世園江世貴200251SPERMATOZOABIOLOGYOFPEAITLOYSTERSPTERIIDAEANDITSAPPLICATL0NT0TRANSGENLCTECHNOLOGYABSTRACTTHEPARENTSOFPINCTADAMARGARITIFERA，PETRIAPENGUINANDPINCTADACATAWEREDISSECTEDFORSPERMATOZOACOLLECTION，ANDTHEEXPERIMENTSOFACTIVATINGSPERMATOZOAWERECONDUCEDUNDERDIFFERENTPH，SALINITYANDWATERTEMPERATUREFRW．THERESULTSSHOWEDTHEACTIVATINGCONDITIONSFORTHESPERMATOZOAFROMDISSECTEDPINCTADAMARGARITIFERAWEREBELOWSUITABLEPHWAS9．510．0，THEOPTIMUMPHWAS9．5SUITABLESALINITYWAS2535；SUITABLESEAWATERTEMPERATUREWAS2530℃．THEACTIVATINGCONDITIONSFORTHESPERMATOZOAFROMDISSECTEDPETRIAPENGUINPARENTSWEREBELOWSUITABLEPHWAS9．09．5；SUITABLESALINITYWAS2535，THEOPTIMUMSALINITYWAS30；SUITABLETEMPERATUREWAS2530。C．THEACTIVATINGCONDITIONSFORTHESPERMATOZOAFROMDISSECTEDPINCTADAFUCATAPARENTSWEREBELOWSUITABLEPHWAS9．09．5，THEOPTIMUMPHWAS9．OSUITABLESALINITYWAS2535，THEOPTIMUMSALINITYWAS3035；SUITABLETEMPERATUREWAS2530。C，THEOPTIMUMTEMPERATUREWAS25。C．THEARTIFICIALINSEMINATIONCONDITIONOFPINCTADAFUCATABYMEANSOFDISSECTINGPARENTSWASSTUDIED．THERESULTSSHOWEDTHATTHESUITABLECONDITIONSFORARTIFICIALINSEMINATIONINAMMONIASEAWATEROF28．6“CWHILESELECTINGEGGSANDSPERMATOZOAFROMTHEMATUREPARENTSDISSECTEDWTJRE；RATIOOFFEMALEANDMALE5191，THEOPTIMUMRATIO7L；PH8．59．0；SALINITY3035，THEOPTIMUMSALINITY30．EFFECTOFPARAMETRESOFPULSEELECTROPORATIONSYSTEMOFGENEPULSERIIRFMODULEINCLUDINGTOTALJOLTS，BURSTDURATION，NUMBEROFBURSTSANDFREQUENCYONACTIVATIONRATEANDFERTILIZATIONRATEOFPINCTADAFUCATASPERMATOZOAELECTROPORATEDWASSTUDIED．CHOOSINGTHATBOTHTHEACTIVATIONRATEANDFERTILIZATIONOFTHESPERMATOZOAELECTROPORATEDWEREABOUT50％ASSTANDARD,THEOPTIMUMPARAMETERSOFELECTROPORATIONINTRANSGENEOFPINCTADAFUCATAUSINGGENEPULSERIIRFMODULEWEREDETERMINED．WHICHWEREBELOW．TOTALVOLTSWAS200V,BURSTDURATIONWAS1．ORES，NUMBEROFBURSTSWAS5ANDFREQUENCYWAS30K50KHZ．FOREIGNDNAWASINTRODUCEDINTOTHEGENOMEOFPINCTADAFUCATABYELECTROPORATIONOFSPERMWITHPULSEELECTROPORATIONSYSTEMOFBAEKON2000MODEL．SPERMATOZOAOFPINCTADA扣加以WASINCUBATEDWITHLINEAR“ALLFISH”GROWTHHORMONEGENEPCAGCGHFOR30MIN．THENMATUREEGGSWEREFERTILIZEDIAVITROWITHTHESPERMCELLSTREATEDWITHELECTROPORATIONATLOKVAND2。PULSESFORTENCYCLES．DNAWASⅡ