高通量引物評(píng)價(jià)系統(tǒng)—PrimerEvaluator.pdf

1、中南大學(xué)碩士學(xué)位論文高通量引物評(píng)價(jià)系統(tǒng)—PrimerEvaluat姓名：朱贊華申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：遺傳學(xué)指導(dǎo)教師：夏家輝20050501ABSTRACTWiththepublicationofthelatesthumangenomesequenceandthefurtherdevelopmentofthestudyondisease—associatedgenes，theapplicationofPolymeraseChainRea

2、ction(PCR)hasreachedatotallynewlevelinmoleculargeneticsresearchAndoptimalprimerisakeyfactortoobtainhi曲qualityresultsinthePCRUnfortunately，forprimerevaluation，thereareonlyseveraltoolswithverysimplefunctionCanbeaccessedNon

3、eofthemCallprovidebatchalignmentbetweenprimersandthehumangenome／regularrepeatedsequences，orderiveanumberofampliconsfromthetemplateatonetime，orprovidebatchcalculationofprimerparameters，orevaluatepairsofprimers’interaction

4、witheachotherinonereactionsystemSesearchershavetodothesejobsinatime—consumingandexhaustingwaybyrepeatingsomeproceduresmechanicallyToprovideautomatedbatchevaluationofPCRprimer，wedevelopedaBrowser／Servermodeapplicationwhic

5、hrunsundertheApacheH丌PserverTheapplicationwasnamedPrimerEvaluatorItusesthelatesthumangenomeandotherdatafrominternationalbiologicaldatabases，combineswithDNannotatorwhichisallannotationtoolforregionalgenomicsequencesPrimer

6、EvaluatorusesisPcrandPrimer3asmajordataprocessingcomponentsItcoveredalmostallaspectsofprimerqualityjudgmentandmanagedtomakeallfunctionsmentionedabovecomeintealityFurthermore，it’Squiteeasytouseandprovidesdetailedinformati

7、onaboutprimersinseveraldifferentformats，whichcanbeeasilyunderstoodandimportedintodatabasesThisprojectappliesbioinformaticstechnologytoexperimentofourresearchAnovelinformaticstoolfordataacquisition，dataanalysisanddatastor

8、ageisdevelopedAfullyfunctionalprimermanagementsystemhasbeenestablishedItbrokethe“bottleneck’ofassaydesignHopefullyitcouldacceleratetheprogressofmoleculargeneticsresearch，especiallythediseaseassociatedgeneresearchPrimerEv