光周期對(duì)不同秋眠類型紫花苜蓿phyA、phyB mRNA和植物激素含量的影響.pdf

1、河南農(nóng)業(yè)大學(xué)碩士學(xué)位論文光周期對(duì)不同秋眠類型紫花苜蓿phyA、phyBmRNA和植物激素含量的影響姓名：吳鵬舉申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：草業(yè)科學(xué)指導(dǎo)教師：王成章201106河南農(nóng)業(yè)大學(xué)2011屆碩士學(xué)位論文EffectsofPhotoperiodonphyAmRNA、phyOmRNAandPlantHormonesinDifferentMedicagoSativaFallDormancyVarietiesSupervisor：ProfWa

2、ngChengzhangPostgraduate：WuPengJu(HenanAgriculturalUniversity)Abstract：Alfalfaasthemainlegumes，itsyieldhasbeenespeciallyconcemedbypeople，falldormancyainfluencefactorofyield，hasbecomethemajorobjectofourstudyAIfalfaWaslong

3、dayplant，sunshinebecomeshortercanaffectitsgrowthduration，thisindicatedthatalfalfahadphotoperiodiceffect，phytochromeisthemainphotoreceptoritisspeculatedthatphyA，phyBgenewerethemajorphotoreceptorsofalfalfafall—dormancyTher

4、eforeitisimportantforUStostudytheeffectofRNAcontentofphyAandphyBandplanthormonesIAA，GA3，ZRABAonfall一dormancyInthisstudywechosenthreestandardvarietiesfalldormancycultivar洲orseman)，semifalldormancycultivar(Archer)nonfalldo

5、rmancycultivar(Cull01)asthetestmaterial，4replicatesweresetforeachcultivar，samplinginfourdifferentphotoperiodictimeofJune，August，SeptemberNovemberrespectively，andleafcollectionWascommencedfromthesecondfilmoftheterminalbud

6、leafcollection，thecontentofphyA，phyBmRNAandthechangeoffourplanthormones(IAA，GA3，ZRandABA)weremeasuredTheresultswereasfollows：Testone，theinfluenceofphotoperiodonphyA，phyBmRNAcontentofdifferentcultivarsoffalldormancyTheres

7、ultSshowedthat：RespectivelyinJune，August，SeptemberNovember，thedifferenceofthePhyAcontentbetweenofNorsemanandArcherwassignificant(Psemi—falldormancy(Arche)non—dormancy(CuflO1)Conclusion：withtheshorterdurationofsunshine，ph

8、yA，phyBcontentincreasedgraduallyindifferentvarieties，phyBcontentincreasedwiththeenhancementoffaildormancyTesttwo，theeffectofphotoperiodonlAA，GA3，ZR，ABAcontentofdifferentvarietiesoffalldormancyTheresultsshowedthat：IAAGA3。

9、ZRcontentofthreetestedvarietiesexisteddifference；nJune，August，SeptemberNovember，andshoweddecreasingtrend，ABAcontentwasdifferencebuthadthegrowingtrendIndifferentmonths，hormonecontentofthreevarietiesweredifferent，buthadnos

10、ignificantvariationConclusion：withtheshorterdurationofsunshine。IAAGA3，ZRcontentdecreased，ABAcontentincreasedIAA，GA3，ZRplayedtheantagonismfromABA，andthecontentwerenegativelycorrelated，thefourplanthormonesworkedcommonlyint