玉米耐旱相關候選基因dhn2與表型性狀的關聯(lián)分析.pdf

1、中國農(nóng)業(yè)科學院碩士學位論文玉米耐旱相關候選基因dhn2與表型性狀的關聯(lián)分析姓名：趙曦申請學位級別：碩士專業(yè)：作物種質資源學指導教師：黎裕王天宇20080601AbstractAssociationanalysisisallimportantwaywhichCanindentifyfavorableallelesassociatingwithdroughttoleranceincorngermplasmresources，providin

2、gtechnicalsupporttobenefitgermplasmenhancementandgeneticimprovementteducethenegativeeffectofdroughtoncornproductionAmaizeassociationmappingpopulationwhichisconsistedof95maizeinbredlinesWasusedforevaluatingsequencediversi

3、tyandlinkagedisequilibrium(LD)ofdhn2，acandidategeneofdroughttoleranceinthestudyPhenotyposrelatedtodroughttolerancewerealsocharacterizedMoreoverbasedontheconsiderationofinfluenceofpopulationstnlcture，associationanalysisof

4、dhn2polymorphismanddroughttolerancephenotypesWasconducted，hopingtoidentifyfunctionalallelesofdhn2geneMainresultsareasfollows1Sequencepolymorphismofdhn2Wasevaluatedusing75inbredlinesoftheassociationpanelTotally37polymorph

5、icsiteswereidentifiedinthecodingregionof75sequencesobtained，including20SNPs(oneper48bp)and15Indels(oneper64bp)Amongthesepolymorphicsites，twositeswereinthefnstintronregiononlyaccountingfor54％HighernucleotidediversityWasob

6、servedintheexonregions，andreducedrapidlyinotherregionsLinkagedisequilibriumdecayedrapidlywithin500bpinthe砌，口region，andthereexistedlinkagedisequilibriumamongmultipolymorphicsites2Correlationanalysisshowedthatanumberoftrai

7、tsincludingkernellength，earlength，eardiametereardry—weight，kernelnumberperrowand100kernelweightcouldinfluenceyieldespeciallyeardryweight(Pearsoncorrelationcoefficientsis096861，P00001)And100一kernelweightshowedsignificantc

8、orrelationwitheardiameterandeardry—weightTherefore，thechoiceofinbredlineswithlongerearandkernel，thickerear’morekernelnumberperrowhighereardryweightandhigher100kernelweightareoptimaltogainahigheryield3Sevensignificantasso

9、ciationsbetweenpolymorphismandphenotypictraitswereidentifiedbyassociationanalysisAllthreeSNPwefeC／GmutationUnderdroughtconditions，oneSNPmutationfromCtoGassociatedwiththeincreaseofchlorophyllcontentTheothertwoallelesassoc

10、iatedwiththeincreaseofearlength，earlength，eardiametereardryweightorkernelyieldMeanwhile，twolnDelallelesincluding3bpinsertionassociatedwiththeincreaseoftassellength，andoneInDelwithlbpinsertionassociatedwiththeincreaseofea