活性真菌對(duì)重金屬離子吸附及抗性的實(shí)驗(yàn)研究.pdf

1、湖南大學(xué)碩士學(xué)位論文活性真菌對(duì)重金屬離子吸附及抗性的實(shí)驗(yàn)研究姓名：周娜申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：環(huán)境工程指導(dǎo)教師：劉云國(guó)20081120AbstractThemetalbiosorptionisaphysico—chemicalphenomenonbasedonabsorption，ionexchange，complexationormicroprecipitationBiosorptionofmetalshasbeenrecentlyr

2、eceivingagreatdealofattentionforbothitsscientificnoveltyandapplicationpotentialinenvironmentalprotectionorrecoveryofpreciousmetalsManyinvestigationshaveshowedthatintactmicrobialcells，livingordead，andderivedmicrobialprodu

3、cts，canbehighlyefficientabsorptionofmetalions，especiallyfromdilutedexternalconcentrationTherehavebeenanumberofreportsonthebiosorptionofheavymetalsHowevertherehavebeenonlyafewreportsonlivingepiphyteabsorptionAstainofresis

4、tancetocopperandzincwithhighconcentrationwasisolatedfroImsoiloftiaolininHuNanprovinceWiththeidentificationof26SrRNAD1／D2genesequence，thestrainwasidentifiedasAspergillusaculeatusThisexperimentusedthegrowingfungusasadsorbe

5、nt，andCu2，Zn2asadsorateTheinfluencesofexperimentalconditionssuchastime，pHvalue，temperature，inditialconcentrationofadsorbentandadsorateonadsorbingeffectarestudiedManyheavymetalsarethenecessaryelementsforthemicroorganismsB

6、utwhentheconcentrationsoftheintercellularheavymetalsaretoohigh，themetalsbecomepoisonousThemicroorganismscanminishthedestroyofthetoxicitybyalterthecomponentialofthemembraneandenhancethesuperficialenrichmentMetalresistance

7、ofmicroorganismsmayenhancetheirabilitytoliveinbadsurroundingsThistextmakedsuretheCu”，Zn2to2拌fungus’minimalinhibitoryconcentration(MIC)UsingtheelectronmagnifierandFTIRtechnicanalysedthenormalandabsorptionmetals’fungusFurt

8、hermore，themeathofiodimetrieestimationwasusedtoanalysethecontentsofglutathionewhichwereculturedindifferentmediumsPrimeryresearchthemechanismofMetal—resistancebyfungusItsoptimumgrowthconditionsweretemperature30。C，pH50，ini

9、tialconcentrationofCu2andZn250mg／L，rateoftheshaker120r／rain，culturedtime120h，theadsorptionefficiencycouldreach54％and60％respectivelyItcanresistcopperof400mg／Landzincof800mg／LTheelectronmagnifierphotosshowthatfunguscellsap