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1、SequenceVariationAnalysisofHeightrelatedGeneDwarf3andDwarf9andTheirAssociationwithGrainYieldRelatedTraitsinMaize(ZeamaysL)ByLiangPanADissertationSubmittedtoYangzhouUniversityinPartialFulfillmentoftheRequirementfortheMast

2、erDegreeofAgriculturalScienceSupervisorByProfChenwuXuAndAssociateProfZefeng碭，轡YangzhouUniversityYangzhou，225009，PRChinaNovember2015SequenceVariationAnalysisofHeight—relatedGeneDwarf9andDwarf3andItsAssociationwithGrainYie

3、ldRelatedTraitsinMaize(ZeamaysL)AbstractInthisstudy，adiversenaturalpopulationwith81maizeinbredlineswasselectedtostudythesequencevariationoftwoheightrelatedgenesDwarf)and肌口，聲Basedonthetargetsequencecaptureplatformtechnolo

4、gyofNimbleGen，thetargetsequenceofgene肌a伊and帥日，矽wereresequencedanditsnucleotidepolymorphismsandhaplotypediversitywerefurtheridentifiedFurther，candidategeneassociationstudywasconductedusing13yieldrelatedtraitstodetectthefa

5、vorableallelicvariationsTheresultsareasfollows：1、Sequencepolymorphismanalysisshowedthat，atotalof308sequencevariants，including219SNPsand88IndelswereidentifiedwithinDwarf3gene，andthereare39SNPsingenecodingsequence82SNPsand

6、25InDelswereidentifiedinDwarf)geneamong87maizeeliteinbredlines，including52SNPsingenecodingsequenceLDanalysisshowedthatsomeSNPswereinstrongLDbutsomeSNPsshowweakLDwithothers，thedifferentLDpattemsuggestedthatthesegenes(Dwar

7、f3，上M口矽)maybeundergonedifferentselectionduringthebreedingprocess2、Accordingtotheresultsoftheassociationanalysis，eightSNPsin跳口，筘geneweresignificantlyassociatedwithyieldrelatedtraitsintwoenvironmentsOfthem，SNP_2820(T／G)was

8、significantlyassociatedwithplantheight，whichexplained744％ofphenotypicvariationsItisanonsynonymoussite，whichCanleadtocodingproteinsequencechanges(T／P)EightSNPsitesinDwarf)geneweresignificantlyassociatedwitllyieldrelatedtr

9、aitsSNP1569(A／C)wassignificantlyassociatedwithplantheight，whichexplained638％ofphenotypicvariationsSNP1569(A／C)wasanonsynonymoussite，whichcouldresultaminoacidvarationOnenonsynonymoussiteSNP一975isassociatedwiththeearlength

10、andthelengthofbaretip，itcanexplain785％and693％phenotypicvariationTheseresultssuggestedthatthepolymorphismsinmaize撕口夠andDwarf)locuscouldbeusedinmolecularmarker—assistedselectiontomodifytheplantheightinmaizebreedingprograms

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