可用于中草藥有效成分提取的纖維素酶的固定化研究.pdf

1、重慶大學(xué)碩士學(xué)位論文可用于中草藥有效成分提取的纖維素酶的固定化研究姓名：周熙云申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：藥物化學(xué)指導(dǎo)教師：王遠(yuǎn)亮20091202英文摘要ABSTRACTCellulaSesaree叼rIIlest脅hydrolyzethep一(1—4)linkagesincellulose111ey盯eproduced嬲amulticomponentenzymesystemcomprisedusuallyofthreeenz)，mes：e

2、ndocellulaSes，cellobiohydrolaLses，鋤dp—glucosidasesTheseenzymesfomacellulolyticsystem鋤dhydrolyzeinsolublecelluloseinaVe巧emcientandsyne曬Sticwly，socellulaSes11a_veeno冊(cè)ousbiotedllll0109icalpotemialinVariousindustries，includi

3、ngthephaImaceutical，food，鋤dchemicalengineering，etcWhatisnotewormyspecially，aScellulaLsescalldecomposemecanlpaCtstnIctureofVegetalceU、Ⅳalla11dresultiIlt11edissolutionofe毹ctiveherbalingredients，maIly舭shaChievememsinextmcti

4、ngherbalingrediemsbycellulaSehaVebeenmadeinrecentyearsNeVertheless，tllepractical印plicationofcellulaSesisstillfairly1imitedThemainreasonist11ehighcostofenzymebecausefkecellulaSesaresusceptibletoiIlaCtiVationaLs、veUasdi銜cu

5、lttoisolation，purificationandrecyclesⅥlriousmethodslIaVebeenreportedforimmobilizationofceUulasesAcr)rlicpolylnerswIlichconlainactiVe毋oupsaresui訕leforimmobilizationenzymesInmysmdy，polymethacr)，licacidgelandpoly(ac巧licacid

6、—co—hydroxyemylmethacrylate)ma印eticmicrospheres、ⅣeresynthesizedCellulases、Verei刪mobilizedontothesecarriersThee虢ctsofamountofcellulases，di腩rentbu行erpH，immobilizationtiIIle，concentlationofactivatingagentonme鋤ouIltofenzymel

7、oading，enZymeactiVi壩specificandretainedactiV時(shí)ofimmobilizedemEylnewereiIⅣestigatedSomecharacteristicsofimmobiliZed砒ld丘eecellulaSes，suCh嬲optimumtemperature，optim啪pH，Michaelisconst趾t，methemlal，operationalarldstorages訕ilitie

8、swereinVeStedandcomparedThem曩incontentsareincludedasfollows：1Cmss—lin玉【ingpolymethacrylicacidgel、^，aSsyI】血esizedCellulaseswereimmobilizedontot11iscarrierbyadsorption，coValentcouplingaIldcrosslilll(ing，mldtheimmobilizatio

9、ncorlditionsareoptimizedThe鋤ountofenzymesloadingofimmobilizedenzymesbyadsorption，coValentcouplingaIldcrosslinkingis19，14and1Om∥g，andt11eactiVit)，retentionis456％，308％a11d228％，respectiVely2SomecharaCteriSticsofcellulaSesim

10、mobilizedonPMAAaremVestigated111eoptim啪temperatureofimmobilizedcellulaSesrisesby1O℃TheoptimumpHofimmobilizedcellulasesbyadso叩tion2uldcoValentcouplingis56and66respectivelyImmobilizedcellulaSesbycrosslinkinghaSrelatiVely址g