豬發(fā)情周期雌激素受體α（ERα）基因表達(dá)豐度及E區(qū)cDNA克隆研究.pdf

1、揚州大學(xué)碩士學(xué)位論文豬發(fā)情周期雌激素受體α（ERα）基因表達(dá)豐度及E區(qū)cDNA克隆研究姓名：丁利軍申請學(xué)位級別：碩士專業(yè)：動物遺傳育種與繁殖指導(dǎo)教師：丁家桐20050601Abundanceanalysisofestrogenreceptoralpha(ERa)mRNAinporcinethroughoutthemenstrualcycleandexpressingofEdomaincDNAMScandidate：DingLijunSu

2、pervisor：ProfDingJiatong(AnimalScienceandTechnologyCollege，YangzllouUniversity225009)AbstractXiaoMeishangiItswithdifferentEI池genotype(hA，AB，BB)identifiedbyRTPCRtechnique，wereslaughteredtostudyreproductiveparametersNodiff

3、erenceinovaryweightuterusweight，ovarylength，ovarywidthanduterinehornlengthweredetected(PO05)EstruscyclelengthwasnotaffectedbyERagenotype(PO05)Furthermore，nipplenumberswerenotdifferentforthreegenotypes(P005)Inordertoinves

4、tigatelocalizationandquantitativeexpressionofERamRNAinovaryanduterusofporcineonDay2，7，12，17throughoutmensmmlcycle，nonradioactiveinsituhybridizationusingDIGlabelledoligonucleofideprobeswasappliedIntheporcineovarypositives

5、ignalsforERawerelimitedexclusivelytothegranulosecellsofalltypesoffolliclesandwerepresentinafewcellsofearlycorporaluteaExpressionofERamRNAinovaryremainedlOWduringthewholeoestruscyclebutincreasedonDay17ERapositivesignalswe

6、remainlydetectedinglandularepithelialcells，afeWpartofstromalcellsandsmoothmusclecellsintheuterusChangesofERamRNAinporcineendometrittmwerehighlymenstrualcycledependentExpressionofERamRNAonDay17oftheocstruscyclewassignific

7、anthigherthanthatofDay12rPO01)E2concentrationsofserumweremeasuredbyradioimmtmoassy(RIA)onDay2，7，12，17ofthemenstrualcycleThelevelsofERamRNAsynchronized、viththechangesofestrogenwithpositivelyrelations(P20042)PartialEdomain

8、eDNAforporcineERagenewasamplifiedfromXiaoMeishan‘piguterusTheeDNA，546bp，encodedapeptideof182aminoacidswithapredictedmolecularweightof211KDaandpotentialestrogenbindingabilityTheaminoacidsequenceoftheporcineERaEdomainwas97

9、3％，978％，967％，and951％identicaltothatofhuman，cattle，sheepandchickenTheporcineERageneEdomaincDNAwasamplifiedfromrecombinantplasmidpGEMTERaEusingPCRtechnique，andthentransclonedintotheprokaryoticexpressionvectorpGEX一6p一1ERaEf