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1、石河子大學(xué)碩士學(xué)位論文準(zhǔn)噶爾雅羅魚的核型和帶型研究姓名:王佳君申請(qǐng)學(xué)位級(jí)別:碩士專業(yè):生物化學(xué)與分子生物學(xué)指導(dǎo)教師:胡文革201106IIIAbstractLeuciscusmerzbacheribelongstoCyprinifmes,Cyprinidae,Leuciscinae,Leuciscuscuvier,alsoknownasXinjiangLeuciscus.Inthedivisionofthewldgeographical
2、distributionLeuciscusmerzbacheriistheuniqueindigenousfishinXinjiangonlyfoundintheJunggarBasinofXinjiangTianshannthernriversystems.Thefoodvalueofthefishhashighnutritionwhichfmerlywasthemaineconomicalfishinproductionareash
3、ashugeprospectsfartificialbreeding.SincemanyyearsbecauseofvariousreasonsthenatrualresourcesofLeuciscusmerzbacherihasbeendecliningitsextantnumberisalreadyextremelyscarcefacedwithendangeredconditionTherefeithastheimptantsc
4、ientificresearchvalue.Inthispaper,thestudyongeicdiversityofthekaryotypesbingpatternsofthemhasnotbeenreptedthusfar.BasedonthesecurrentstatusthechromosomekaryotypebingpatternsofLeuciscusmerzbacheriwerestudiedcomparedwhichw
5、erecollectedfromJingheKuitunregions.InthisstudythechromosomekaryotypebingpatternsofLeuciscusmerzbacheriwhichwerecollectedfromJingheKuitunregionswerecomparedbykaryotypetechniqueAgNs,CBGBsoastoanalyzethevariationonthelevel
6、ofcytogey(chromosome),WepreliminarilyanalyzedthechromosomediversityofL.merzbacheri.ThechromosomespecimensofLeuciscusmerzbacheriwereobtainedfrommetaphaseofheadkidneykidneycellsbycolchicinelowconcentrationairdryingtechniqu
7、ethenstudiedtheirGiemsastainAgNs,CBGBwerestudiedinthispaperThemainresultsofthestudywereasfollows:1L.merzbacherifromJingheKuitunhadadiploidchromosomenumberof2n=50itskaryotypefmulawas18m14sm6st12tNF=82sexchromosomewasnotfo
8、und.ThepossibleevolutionaryrelationsinchromosomewerediscussedamongLeuciscusmerzbacheriLeuciscusbaicalensisLeuciscusidus2TheAgNspolymphismsofL.merzbacherifromJinghewereindividuallyspecific1~2ofthenumberthefrequencyof1AgNs
9、wasthelowest(10%)whilethefrequencyof2AgNswasthehighest(70%).ThemainAgNspolymphismswerelocatedintheshtarmsofthechromosomem1m4.TheAgNspolymphismsofL.merzbacherifromKuitunwereindividuallyspecific1~2ofthenumberthefrequencyof
10、1AgNswasthehighest(68%)whilethefrequencyof2AgNswasthelowestt(25%).ThemainAgNspolymphismswaslocatedintheshtarmsofthechromosomem3.TheassociationsofAgNswerenotobserved.Thepossibleevolutionaryrelationsinchromosomewerediscuss
11、ed,theresonsofthedifferencewerealsodiscussed.Theresultsshowed:L.merzbacherifromJinghehadahigherevolutionarydegree3ThereweresomedifferencesintheCbingpatternsamongpopulationsinJingheKuitunwhichincludedthedifferentlocationn
12、umberofCb.Therewere1~4CbsonthechromosomesofL.merzbacheri2fthemostones.TheCbingpatternwasmainlyconsistedofcentromericbtelemtricbintercalaryb.Theresultsshowed:L.merzbacherifromJinghehadahigherevolutionarydegree,whichwasacc
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