波紋唇魚的核型和線粒體全基因組結(jié)構(gòu)分析.pdf

1、海南大學(xué)碩士學(xué)位論文波紋唇魚的核型和線粒體全基因組結(jié)構(gòu)分析姓名：霍蕊申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：水產(chǎn)養(yǎng)殖指導(dǎo)教師：張本尹紹武20090601AbstractCheilinusundulatesbelongstoCheilinusgenus(Osteichthyes：Actinopterygii：Perciformes：Labroidei：Labridae)Cundulateshavebeeninthelistofendangeredspec

2、iesfourtimessince1996to2005byIUCN，WWECITESTherefore，theresourceconservationandenhancementofCundulatesareimportantButthestudyongermplasmresourcesofCundulatesarerarelyThegermplasmresourcesofCundulateswasanalyzedinthispaper

3、byusingcytogeneticalandmolecularbiologymothodsdesignedprovidingmorebaseinformationforgeneticsandbreedingofCundulates，theendangeredfishThemainresultsareasfollows：1ToexplorethecellbiologicalcharactersofCheilinusundulates，w

4、eanalyzedthekaryotypeofCundulatesfromtheirrenaltissuesbythePHAandcolchicineinjection—airdryingmethodTheresultsshowedthattherewere48chromosomesinCundulatesanditskaryotypeformulaWas2n=48=6m42t，NF=54Satellitechromosome，seco

5、ndaryconstrictionsandsexchromosomewerenotfoundinCundulatesThekaryotypeofCundulatesareinaccordancewiththehighergroupoffishevolutionarytaxonomy2By23pairsofprimersusedinPCRandsequencing，weobtainthecompletemitochondrialgenom

6、esequencesofCundulatesThemitogenomelengthofCundulatesis16602bpAcomparisonofthismitochonidrialgenomicsequencewiththoseofthevertebratemitochondrialgenomesrevealsasimilargeneorderandcompactmorphologicorganizationThegenomeco

7、ntentsconsistofthe13protein—codinggenes，tworRNAs，22tRNAsandonecontrolregionItishighlyhomologoustootherichthyiccounterpartsThissequenceisthefirstcompletemtDNAsequencesofCheilinusgenus，3Inthispaperwesummarizedbiologicalcha

8、rateristicsofgeographicaldistribution，ecologygrowthandreproductionofCundulatesandthemajorresearchprogressongermplasmresourceofmorphologychromosomekaryotype，biochemicalgeneticsandmoleculargeneticsofCundulatesMeanwhile，the