干旱脅迫下水稻生殖發(fā)育相關(guān)分析.pdf

1、復(fù)旦大學(xué)碩士學(xué)位論文目錄中文摘要............……，............................……nABsTARcT.，..................................................……班綜述........................................................……1一水稻干旱脅迫研究進(jìn)展.......................

2、...........……1二水稻花器官發(fā)育研究進(jìn)展.................................……3三結(jié)論與展望............……，.........一……7第一節(jié)水稻材料的種植與干旱處理二，.，.……，.…，.......……，.…9一材料與方法........……，............……9二結(jié)果與討論...……，.…，.............................……10第二節(jié)水稻

3、花收集、RNA抽提、芯片雜交與結(jié)果分析.……，……，……12一材料與方法.........................................……12二結(jié)果與討論............................................……17第一部分干旱脅迫下的減數(shù)分裂期花的轉(zhuǎn)錄組分析........……17第二部分不同發(fā)育時期的花在干旱脅迫狀態(tài)下的表達(dá)分析..……33第三節(jié)Real一TimePCR驗證基因芯

4、片結(jié)果可靠性.................……41一材料與方法....................................一41二結(jié)果與討論............................................……44第四節(jié)水稻干旱相關(guān)基因的克隆與轉(zhuǎn)化..........................……47一材料與方法.........................................

5、..……47二結(jié)果與討論........……，，二，……，二，，，，，二，....……，.53第五節(jié)討論....................................……56，曰?！へ?參考文獻(xiàn)致謝…復(fù)旦大學(xué)碩士學(xué)位論文ABSTRACT死ce15oneofthemostimPortanteroPsinchinaandevenintheworld.Inrecentyears，droughthaseausedsevererice

6、yield1055，esPeciallyinthosewater一deficitareas.ImProvingrieegrov注hdeveloPmentunderdroughtstress15imPtantfmaintaininggrainyield，PreviousresearchmainlyfoeusontheeffeetofdroughtonrootandIeafdeveloPmentduringvegetativegrowths

7、tage.However，flower，asthereProducti、，eorgan，Wil]direetlya價ctthegrainProduetion.JtremainsunelearhowricefloralorgansresPondtoWater一denciteonditionandtherefealterthereProduetivedeveloPment.Ourresearchaimstounderstandthemole

8、eularmeehanismsregulatingthisProcess，andfinallytoimProvericeyieldunderdroughtstress.ResPonsesofricefloretstodroughtstressatthemoleeularlevelaroundmeiosisstagewerestudiedinordertoobtaincandidategeneswhichcanimProverieedro

9、ughttoleranee.WetreatedtherieePlantsatearlyreProduetivestageswithlesswaterthannmal，thenholdthesameSR認(rèn)廠C(5011relativewatereontent)faboutsevendays.Afterthat，weeolleetedthedrought一treatedandcontrolrieeflowersaccordingtothet

10、helengthofflets.RNA15extractedfmieroa二ayanalysis.Byanalysisofthemicroarraydata，wefoundthatsomegenesandPathwayshavebeeninducedorinLhibitedunderdroughtstress，suggestingthattheeellstatusofrieeflorets，suchasenergymetabolism，

11、oxireduetionstatUshavebeenaltered.Thegene一ehiPanalysise卻erimentswererePeatedintriplicate.339geneswerefoundtoberePeatedlyuP一regulatedand118genesdown一regulatedunderdroughtstress.W七furtherelassifythesegenesaeedingtotheirGOa

12、ndannotations，aswellastheinfmationfromPublishedPaPers.TheresultsshowthatsomeofthegeneshavealreadybeenrePortedtobeinvolvedindroughtresistance，andothersarenew.SomegenesareinvolvedinmultiPlePathwayssuchellmetabolismandflowe

13、rdeveloPement.MoreexPerimentsWillbenecessarytofurthereharacterizetheirrolesunderdroughtstress.Real一TimePCRwasusedtoeonfirmthemieroarrydata，andtheresultsaregenerallyconsistent.Basedontheanalysisresults，severalgeneswereI!!