玉米種質(zhì)更新過程中遺傳完整性變化的影響因素研究.pdf

1、中國農(nóng)業(yè)科學(xué)院碩士學(xué)位論文玉米種質(zhì)更新過程中遺傳完整性變化的影響因素研究姓名：馬倩申請學(xué)位級別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：盧新雄20050601AbstractApplicationofsafeconservationandscientificrenovationtomaintainthegeneticintegrityofgermplasmresourcesingenebankisthemostimportantissuefor

2、germplasmconservationItisofgreatsiginificancetostudyfactorsresponsibleforgeneticintegritychangeofgenetically’heterogeneouspopulationintherenovationprocessSeedsofsixmaizelandraces(Bendibaiyumi，Huangxiaoyumi，Xiaobaisu，Ando

3、nghuang，TiaohuanuoandJiahongzao)wereanalyzedmorphologicallyandassayedwithSSRmarkersinthisworktostudytheeffectofseedaging。renovatedpopulationsandthesamplingmodelonthealterationofgeneticintegritySO∞toprovidescientificandth

4、eoreticalbasisformakingacriteriaforregeneratinggermplasmresourcesMainresultsfoundwereasthefollowings1、Geneticalterationofsixmaizelandraces(Bendibaiyumi，Huangxianyumi，XianbaisuAndonghuang，TiaohuanuoandJiahongzan)havingdif

5、ferentgerminationlevelswasanalyzedbyusingagronomictraitsTheseedaginghadnotanyobviouslyinductiveinfluaneesOnthemorphologicatraitsofplantheightearheight。earlength，eardiameterrownumberperearleafnumberofmainculm，stemdiameter

6、etc2、Geneticalterationofseedsofmaizelandrace，Xiaohaisu，Wasanalyzedusing5ISSRmarkersTherewerethreesourcesofseeds，onewasconservedintheNationalCropGenebankwithhighgerminationpercentageanditsfilialgenerationafterregeneration

7、andtheotheronewasthefilialgenerationofseedswithlowgerminationpercentagefromShanxiAgriculturalSciencesItshowedthatthenumberofpolymorphiclociandpercentageofpolymorphiclociofthefilialgenerationafterregenerationweresamewitht

8、heseedsbeforeregenerationThenumberofpolymorphiclociandpercentageofpolymorphiclociwere45and8824％ThefilialgenerationsofseedsconservedintheNationalCropGenebankandfromShanxiAgriculturalScienceslost8and11alleles，respectivelya

9、ndgeneratedtwonovelallelesTheallelefrequencyanddendrogramcomparisonresultsindicatedthattheeffectofseedagingonoffspring’changeofgeneticintegrityw∞lessthanthesynthesizedeffectofsizeofregenerationpopulation，seedagingandsele

10、ctivestressesinthefield3、Geneticalterationofseedsof200，100and50plantsofmaizelandrace，Tiaohuanuo。Wasanalyzedusingagronomictraitsand50SSRmarkersWitlthedecreaseofsizeofregenerationpopulation。thekindsofseedcolorreduced，andth

11、eproportionofredbleadsheathplantsdecreasedwhilethewhitebleadsheathplantsincreasedThenumbersofallelesandpolymorphiclociandthepercentageofpolymorphielociofseedsdecreasedafterregenerationThcregenerationpopulationsof200，100a