塔里木大學(xué)2013屆應(yīng)用化學(xué)專業(yè)畢業(yè)生畢業(yè)論文

1、塔里木大學(xué)2013屆應(yīng)用化學(xué)專業(yè)畢業(yè)生畢業(yè)論文目錄摘要....................................................................................................................................................1關(guān)鍵詞.........................................

2、.......................................................................................................10引言.................................................................................................................

3、...............................21實(shí)驗(yàn)儀器和方法............................................................................................................................41.1儀器..................................................

4、...................................................................................41.2試劑...................................................................................................................................

5、..41.3材料......................................................................................................................................41.4方法........................................................................

6、.............................................................41.4.1昆侖雪菊的黃酮含量測(cè)定......................................................................................41.4.3黃酮的抗氧化測(cè)定..........................................

7、........................................................51.4.3.1清除超氧陰離子自由基能力的測(cè)定...........................................................51.4.3.2黃酮溶液清除羥自由基能力測(cè)定............................................................

8、....51.4.3.3對(duì)DPPH自由基清除活性的測(cè)定..............................................................61.4.3.4抗脂質(zhì)氧化能力測(cè)定...................................................................................62實(shí)驗(yàn)結(jié)果與討論......................

9、......................................................................................................62.1昆侖雪菊的黃酮提取和含量測(cè)定......................................................................................62.1.1蘆丁標(biāo)準(zhǔn)曲線繪制

10、結(jié)果...........................................................................................62.1.2樣品測(cè)定結(jié)果..........................................................................................................72.2黃酮的還原

11、性測(cè)定結(jié)果......................................................................................................72.3黃酮的抗氧化測(cè)定結(jié)果...................................................................................................

12、...82.3.1清除超氧陰離子自由基能力的實(shí)驗(yàn)結(jié)果...............................................................82.3.2清除羥基自由基能力實(shí)驗(yàn)結(jié)果...............................................................................92.3.3對(duì)DPPH自由基清除活性的實(shí)驗(yàn)結(jié)果..........

13、......................................................102.3.4抗脂質(zhì)氧化能力實(shí)驗(yàn)結(jié)果....................................................................................123結(jié)論...........................................................

14、...................................................................................12參考文獻(xiàn)...................................................................................................................................

15、.......13致謝..........................................................................................................................................14塔里木大學(xué)2013屆應(yīng)用化學(xué)專業(yè)畢業(yè)生畢業(yè)論文1昆侖雪菊總黃酮抗氧化活性昆侖雪菊總黃酮抗氧化活性研究研究摘要【目的】采用超聲萃取法用四種不

16、同的溶劑提取昆侖雪菊中總黃酮類化合物，測(cè)定其含量并研究其抗氧化性?！痉椒ā坷锰J丁標(biāo)準(zhǔn)曲線對(duì)黃酮的含量進(jìn)行測(cè)定，采用DPPH法，結(jié)晶紫分光光度法，鄰苯三酚自氧化法，抗脂質(zhì)法研究總黃酮的抗氧化能力，用普魯士藍(lán)法對(duì)其還原能力進(jìn)行研究?！窘Y(jié)果】70%乙醇為溶劑提取率最高，石油醚為溶劑提取率最低。雪菊總黃酮有清除超氧負(fù)離子自由基，羥基自由基，DPPH自由基的能力，以及較強(qiáng)的抗氧化活性?！窘Y(jié)論】在試驗(yàn)濃度范圍內(nèi)，對(duì)羥基自由基最大清除率為98.9%

17、，對(duì)DPPH自由基最大清除率為94.8%，對(duì)超氧陰離子自由基最大清除率為52.6%，并且抗氧化能力與總黃酮的含量相關(guān)。黃酮作為天然的抗氧化劑，具有廣闊的應(yīng)用前景。關(guān)鍵詞昆侖雪菊；總黃酮；還原性；抗氧化性StudyingontheFlavonoidsinKunlunChrysanthemumofAntioxidantActivityAbstract:【Objective】Expletheoptimalexperimentalconditi

18、onfthemeasureofthetotalantioxidantcapacityofnaturalproductbasedonCe(IV)reducingcapacitybyoptimizingthedeterminingofantioxidantcapacityofchlogenicacidviaCe(IV).【Methods】ThefluescentCe(IV)exhibitedstrongfluescenceatexcitat

19、ionwavelengthof319nm.Themethodisbasedontheelectrontransfer(ET)reactionbetweenCe(IV)ionchlogenicacidsubsequentdeterminationofreducingofCe(IV)ionsbyafluometricmethod.Weusethesinglefactexperimentmethodtooptimizeexperimentco

20、nditionsfromthesolventtemperaturereactiontimePHvaluethemoleratio.【Conclusion】Experimentsshowthat0.3molLofsulfuricacidisthebestsulfatesolventstemperatureisatroomtemperaturereactiontimeat60scanbeastardPHvalueisbetween01the

21、molarratioofisin4.4:1.Thenewmeasureoftotalantioxidantcapacityofnaturalproductbroadapplicationprospect.Thenewmeasureoftotalantioxidantcapacityofnaturalproducthasbroadapplicationprospect.Keywds:KunlunsnowchrysanthemumFlavo