胡楊耐受鹽脅迫分子機制初步研究.pdf

1、V108s77點分類lj：藺卅幺害研究生學位論文南緞論文題口(中文)趔塹匭壁墊鹽迫僉王扭劍塑壟盟究Preliminarystudiesonmolecularmechanismof論文題目(外義)s出!Q!曼!壘墜￡星Q￡￡星綸盤絲叢￡垡石丹究生姓名工摘一學科、專業(yè)擅物堂研究方向植物盆王生物堂學位級別題導帥姓名、職稱韭童逝數(shù)援論文工作起止年月至QQ壘生竺目至2QQ2生壘且論文提交日期2QQZ生三且滄文答辯日期2QQ2生三目學位授予日期——

2、胡楊耐受鹽脅迫分子機制初步研究AbstractPreliminarystudiesonmolecularmechanismofsalttoleranceofPeuphraticaAbstractPopuluseuphraticaoliv，amemberofPopulus，Salicaceae，isfamousforitsabi—litytoendurehighsalinityTherearcmanyrearchesaboutitsphy

3、sicologicalchangesundersaltstress，butlittleisknownonitsmoleculermechanismofsalttoleranceHerewestudiedtheexpressionofPeSOSl(PopuluseuphraticaofivSaltOverlySensitive1)，aputativePeuphraticaNa／Hantiporterandanaly∞dtheelement

4、sratioofdifferentorgansof只euphraticaaftersalttreatmentToaddressthefunctionofPESOSl，complementationstudiesonthesaltsensitiveEscherichiacoilEP432mutantsUainlackingactivityofthetwoNa／HantiportersEcNhaAandEcNhaBwerecarriedou

5、tExpressionofPESOSlsuppressessomeofthesaltsensitivephenotypesoftheEP432TheresultsofNatoleranceconferredbyPESOSlcorrehtedwithlowerNacontentandhigherKcontentToexaminetheexpressionofPESOSlgeneunderstress，RTPCRandWesternblot

6、tinganalysiswereperformedThetranscriptlevelofPESOSlcorrespondingtothetimecoursewasmessuredafler200mMNaCltreatmentsRelativeamountsofthemRNAinsalt—resistant只euphraticaleaveswereinitiallyincensedundersaltstressWesternblotti

7、nganalysisshowedthatPESOSlwasdetectedwithoutstresstreatmentbutwasupregulatedsignificantlybysaltstressinleavesandrootsof只euphratica，afterNaCltreatmentfor12L24hand48hTheexpressionlevelWasnotmuchincreascinthestemsof只euphrat

8、icaToexaminethelocalizationofPESOSlproteininplantcells，weisolatedtheplas—mamembranefractionsandthetoloplsstfractionsfromtheleavesafter200mMNaCltreatments，andcarriedoutWestern—blottinganalysisOurresultsshowedthatPESOSlWas

9、detectedinplasmamembranefractionsbutnotintoloplastfractionsTheresultssuggestthatPESOSlproteinislocatedonPM，whichisaccordancewithourhomologyanalysisToanalysetheelementsratioofdifferentorgansofPeuphraticaaftersalttreat—men