中國野生大豆6個(gè)基因SNP篩選.pdf

1、東北師范大學(xué)碩士學(xué)位論文中國野生大豆6個(gè)基因SNP篩選姓名：尹光申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：遺傳學(xué)指導(dǎo)教師：王興智董英山20090501AbstractSoybean(Glycinemax)isoneofthemaineconomiccropsBecausehi曲yieldingcultivarsdominateproductionbutarerelativelyfewinnumberandaregeneticallysimilar，gen

2、eticdiversityinthesecropsispresumedtohavedeclinedtoalarminglylowlevelsThereductionofgeneticdiversitydoesnotbodewellforfuturegeneticgainsincropproductivityandcouldresultinbroadsusceptibilitytonewlyemergingdiseasesorinsect

3、pests，therebythreateninglongtermfoodandfeedsecurityDuetothehi曲degreeofgeneticdiversity，GlycinesojahasbecomeavaluablegeneticresourceforsoybeflnbreedingInthisstudythreeNmetabolismrelatedgenes：Ammoniumtransporter(AMTl)nitra

4、tetransporter2(NRT2)，aspartateaminotransferase2(AspAT2)；threeresistencerelatedgenes：，ABREBindingProtein9(ABP9)，betainealdehydedehydrogenase(BADH)，calciumdependentproteinkinase13(CPKl3)wereisolatedandclonedusingtheapproac

5、hofmolecularbiologycombinedwithbioinformatics，therelatedinformationofSNPswereobtainedandtheresultswereasthefollowing：1UsingcDNAsequencesofsomeotherspeciesasquerysequences，thehomologousExpressedSequenceTags(EST)assembledc

6、ontigsweresearchedoutonTIGRwebsite；theprimersweredesignedaccordingtothecontigssequences，andpartofsixgeneswereisolated2AllthefragmentoftheeachgenewerealingnedbyusingVectorNTIsoftware2SNPsweredetectedinAMTl，26inNRT2，66inCP

7、Kl3，12inABP93inAspATand27inBADHJustthesameasreportedbefore，thenumberofSNPsinnon—codingregionsisdoubledthatofSNPsincodingregions3StatisticsweregeneratedbyusingDnaSPsoftware50，the7cofAMTl，CPKl3，BADH，ABP9，AspAT2andNRT2are00