濕地溶源性浮游細(xì)菌的生態(tài)分布及其基因多樣性.pdf

1、華中師范大學(xué)碩士學(xué)位論文濕地溶源性浮游細(xì)菌的生態(tài)分布及其基因多樣性姓名：劉妮申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：趙以軍20080501⑧＼：：：：／碩士學(xué)位論文MASTER‘STHESISAbstractInt11iss伽y，廿1einvestigationsontllelysogenicbacterioplanl(tonabundallceindi行erem1akes，rivers，reservoiIs，pondsandotll

2、er夠pesofnaturalwetlandswereconducted’andmeirr巾actofenViromne叫‰torsonmemwerediscuSsedMe鋤w11ile，megeneticdiVers毋ofsomebact嘶oplaIlktons鋤ples、vaSdete肌iIled，andsomespeciesoflysogeIlicbacteriatllateaLsyt0beillducedwereidentifi

3、edTheseresults、Ⅳillpossiblyhelplls缸恤erunderstand也erelation幽pbe觚eenbacterioplanktonaIldvirioplaIlI∞11，嬲wenaStllee晚ctofenviro姍e刪fktorSontllemAseriesofs鋤pleswaScollectcdf幻meutrop陸cfbshwaterlakesofdifI色rent刪tritionallevels協(xié)H

4、ubeiProvincedu血gMay2006toJaIluary2007，nleses鋤plesweretrea：cedwi也mitomyc洫Ctostimulatedpr(’phageiIliluctio璣a芏ldthebacterioDl踟1l∞nabundanceanden啊ronIIlen切1fhctorsweremeasu|_edatthes鋤etimeItwaSobsen，edthatthefIrequencyoflyso

5、gemccells(FLC)waSnosi鰣fic鋤tdi婦Ferencebetv∞ensummera11dwinteroftheyearinmeeu昀p11iclakesButnleFLCiIlhypereutmplliclakes(7478％)Ⅵ砸si嘶ficantly11igIlertllalltllatoflighteu臼opmclakes(2l66％)Acrossallinvestigatedlakes，F(xiàn)LCwaussigl

6、lificantpositiverelatedtoTLIandCOD(po01)nappearedt11atttlerewaSnoco圩elationb咖eenFLCandtotalplanktonabun幽rIce，andttles越neresultwasobservedbel：強(qiáng)他enFLCandte如【peKmJreItwas也enutrientlevelofeutropllicationmatwasthe(1ecisivef如t

7、or血FLCThecomm疵坤s仃uctureofbacterioplan虹onindi丘IerentseaSoIlS，di丘Ierentnutritional1eVelsofthelake—t)，pe、Vetl鋤dsanditschangesaRernleprophagesiIlductionⅥ，ereinvestigatedusingPCR_DGGEtechnologyTheexpe血nentalresuItSshowedthatn

8、lecommulli時stIuctureofeachsites鋤plechangedli仳le謝tllseaSoIl’buttlleabunC‰ceofspeciesmightbev撕anceindi行erentseasonsThere、Ⅳerece疵nchangesinthecommuni夠Stlu曲】reaftertlleprophageillduCtionIIladditio瑪theresultsofoptimalC0nditio

9、nsexperimentshowedtllatelectrophoresisconditionsontheDGGEwouIdhaveadirectimpactonⅡ1eresults，andtlledegenerationof齲adientandttleC0ncentrationofPCRproductsalso射瓷ctedtheresultsThenu】nberofbacteriaplanktonst咖sisolal【edf兩meaC

10、_hla：keswere10oftheNallhuLake，15ofChaohuLake，17ofMiaohuLal(e，10ofShuiguoLake，12ofFen曲uotaiLake111e16SrRNAgenes五_om16stajIlsweresequenced，鋤ongwllich14s慨IlsweremosteaSilyinducedand2straills、ⅣereeasilyiIlducedThehldumionres