兒茶素的吸附層析最佳條件及在膨脹床上的應(yīng)用研究.pdf

1、合肥工業(yè)大學(xué)碩士學(xué)位論文兒茶素的吸附層析最佳條件及在膨脹床上的應(yīng)用研究姓名：董新昕申請學(xué)位級別：碩士專業(yè)：農(nóng)產(chǎn)品加工及貯藏工程指導(dǎo)教師：姜紹通2003.4.1SeparationconditionsofcatechinswithadsorptionchromatographyanditsapplicationtoexpandedbedAbstractThreeadsorbentsofPA，H103andXAD一4weFeusedtOis

2、olatecatechinSfromgreenteawithcolumnchromatographysystemandtheexpandedbedadsorptionchromatographysystemThroughthesystematicinvestigationsofadsoI。ptiveperformance，thermodynamicandkineticcharacteristicsofvariousadsorbents，

3、andtlleelutionperforlrlaneeofvariouseluantsonresolutionweestablishedtheoptimumconditionswhichcouldbeappliedtotheseparationofcatechinseffectivelywithexpandedbedtechnologyTheresultofadsorptionperformanceexperimentshowedtha

4、t，theadsorptionperformanceofPA，H103andXAD4werebettertheirequilibriumadsorptioncapacitywere40506mg／ml，39497mg／mland20877mg／mlrespectivelyThestudyofthermodynamiccharacterizationshowedthattheadsorptionisothermsofthesethreea

5、dsorbentscoulda11bedescribedbytheLangmiurequationAndtheyallbelongtothepreferentialisothermandarehighlyrelatedtotheLangmiurequationBystudyingthekineticcharacterizationanddrawingtheadsorptionkineticisotherms，weknowthat，PAa

6、ndH103aremiddlingspeedadsorbents，whileXAD一4islowspeedadsorbentTheconstantsofequilibriumrateofPA，H103andXAD一4areKpa=1461h～，KHl03=1198h～，KXAD4=0498h‘。inorderTheybecomelowerinsuccessionTheanalysisofpenetrationcurveshowedtha

7、t，themattertransferzoneofXAD4islongest，whilethemattertransferzonesofPAandH103areshorterwhichputgooduseofPAandH103Thestudyoftheelutionperformanceofvariouseluantsshowedthat，theappropriateeluantsforPA，H103andXAD一4toisolatec

8、atechinswere85％ethanol，mixtureof64％methan01308％Acetonitrile一5_3％tetrahydrofuram575％watermixtureofethanol—ethylacetate—water(1：3：】，VJV：v)respectivelyBymakingcontrastiveanalysis，weknowthatthebestseparationeffectCanbeacquir

9、edbyusingPAasadsorbentand85％ethanolaseluantAtiertakingtheconditionoptimizedexperiment，weeventuallychosethebestmethodforcolumnchromatographyasbelow：usePAasadsorbent，use45％and85％ethanolaseluant，eluateintwostepswiththeveloc