用DNA指紋技術(shù)鑒定布氏田鼠種群內(nèi)親緣關(guān)系的初步研究.pdf

1、中國(guó)農(nóng)業(yè)大學(xué)碩士學(xué)位論文用DNA指紋技術(shù)鑒定布氏田鼠種群內(nèi)親緣關(guān)系的初步研究姓名：王登申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：農(nóng)業(yè)昆蟲與害蟲防治指導(dǎo)教師：施大釗20040601AbstractBrandt’svoles(Micn7lusbrandti)isoneofthetypicalspeciesofthepasturerodents，Itsimportanttoestimatethekinshipoftheindividualsinagroupwh

2、eninvestigatingthepopulationdevelopmentTraditionally，thekinshipwasestimatedbytheecologicalmethodsbasedonitsbehaviorthemorphologicalcharacterandphysiologicalcharaterNowbyDNAfingerprinting，wecanaccuratelyidentifythekinship

3、Wecomparedthemolecularmarkerswhichcanidentifythekinshipoftheindividual，includingRFLPRAPD，multilocusminisatellitesandmicrosatellitesDNAfingerprintingandmonolocusmicrosatellitesDNAfingerprinting，withtheresultthatthemonoloc

4、usmicrosatellitesDNAfingerprintingisthebestInthisstudy19microsatellitesIociofthemouse(Musmuseuhmdomesticus)werechosenfromtheGenBank，whichhadbeensubmiaedbytheNuffieldDepartmentofSurgery，8oftheI9lociwerefoundpolymorphic，an

5、dwereusedinthemonolocusmicrosatelliteDNAfingerprintingtoidentifythekinshipamongtheindivivalsinthreegroupsForthethreegroups，therewere2malesandlfemaleparentsinolqegroup，andthekinshipwasclearinanothergroup，whilethekinshipof

6、theindivivalscapturedfromthepasturewasnotclearinthethirdgroupIntheend，thenOBfatherweremadeoutfromthegroupIIII，andtheindividualfingerprintingmapinthegroup11wasfoundaccordingwiththeMendelslawTotheindivivalscapturedfromthep

7、asture，weidentifiedtheparentagesandtheoffspringagesaccordingtotheageandthencomparedthemonolocusmicrosatelliteDNAfingerprintmapandidentifiedtheirkinshipWeconfirmeditwasaccuratetousemonolocusmicrosateUiteDNAfingerprintingt