以F68為模板劑介孔SiO-,2-的合成及其對(duì)脂肪酶的固定化.pdf

1、山東輕工業(yè)學(xué)院碩士學(xué)位論文以F68為模板劑介孔SiO的合成及其對(duì)脂肪酶的固定化姓名：白光偉申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：應(yīng)用化學(xué)指導(dǎo)教師：周國偉20100601山東輕T業(yè)學(xué)院碩l：學(xué)位論文ABSTRACTInacidicconditions，mesoporoussilicanamedMS100andMS130weresynthesizedusingtriblockcopolymerF68astemplateTEOSassilicasource

2、atdifferenthydrothermaltemperatureThesampleswerecharacterizedbyXRD，N2adsorptiondesorption，F(xiàn)TIRandTEM，theresultsshowthatthesesamplesareamorphousmesoporousmaterialswith“inkbottle’porelikestructureThecharactersofthesesample

3、s(MS一100，MS130)weredescribedasfollows：thediametersare47nln，46nm；thetotalporevolumesare0271Cqli3曠1、0723cm3曠1；specificsurfaceareasare7612m2gI、6701m2g1111ehigherthetemperatureofhydrothermalsynthesis，themesoporoussilicahasth

4、ehigherporosityandthemoreuniformofporesizedistributionThesemesoporousmaterialswereusedassupportstoimmobilizetheporcinepancreaticlipase，andtheimmobilizedamountcanreach740mg‘g‘gand760mg’g‘Theoptimumimmobilizedconditionsare

5、atpH=6for6hTheoptimalpHandtemperatureofthehydrolysisoftriacetinfortheimmobilizedPPLareat80and45℃，whilethefreePPLwereat70and35℃ImmobilizedlipasescanmaintainhighactivityinawiderangeindicatingthattheimmobilizedPPLismorestab

6、lethanfreePPLDuetothedisclosureoflipase，theactivityofimmobilizedPPL(MS100PPL、MS130PPL)havebeenfoundtobe50％，65％oftheiroriginalactivityafterthefifth玎娜eMesoporoussilicas(MS—l，MS一2，MSo)withdifferentporediameterweresynthesize

7、dbyhydrothermalmethodusingtriblockcopolyrnerF68astemplate，TEOSassilicasource，anddifferentamountofTIPBWaSaddedintothismixtureasexpandingagentThesesampleswerecharacterizedbyTEM，XRD，F(xiàn)1卜IR，N2adsorptiondesorptionexperiments，a

8、ndtheresultsshowthatthesemesoporoussilicasareamorphousmaterials，theadditionofswellingagentdoesnotchangethecrystalstructureofmesoporousmaterialsHoweverthestructureparametersofmesoporoussilicas，suchasporesizeandporevolumew

9、illbechangedwiththemcreaseofswellingagentamountWimtheincreaseintheamountofTIPBtheporevolumeincreasegraduallywhiletheporediametersincreaseto52llnlandreducedto38nnlThesemesoporoussilicas(MS1，MS—2andMS3)weleusedassupportsto