明膠模板法制備介孔材料及其對脂肪酶固定化的研究.pdf

1、山東輕工業(yè)學院碩士學位論文明膠模板法制備介孔材料及其對脂肪酶固定化的研究姓名：王熙梁申請學位級別：碩士專業(yè)：高分子化學與物理指導教師：周國偉20110601山東輕工業(yè)學院碩士學位論文IABSTRACTAmphousmesopoussilicas(MPS3MPS4MPS5MPS6)withdifferentpediameterweresynthesizedinhydrothermalsolutionwithdifferentpHat100

2、℃f24husingbiodegradablenaturalpolymergelatinasstructuredirectingagentssodiumsilicateassilicasource.ThepreparedmesopoussilicaswereacterizedbyN2adsptiondesptionsmallangleXraydiffraction(SAXRD)Fouriertransfminfraredspectros

3、copy(FTIR)scanningelectronmicroscopy(SEM)energydispersiveXrayspectroscopy(EDS)transmissionelectronmicroscopy(TEM).Theresultsshowedthatthemesopoussilicahadnonrigidwmholelikepestructure.Distributionofpesizeincreased(3.65.1

4、6.313.7nmrespectively)specificsurfaceareadecreased(512.8437.5315.3247.8m2g?1respectively)pevolumereduced(1.3440.9010.7750.768cm3g?1respectively)withthepHvalueofthehydrothermalsolutionincreased.ThesynthesizedMPSwasthenuti

5、lizedassupptsfimmobilizationofPcinepancreaticlipase(PPL)byphysicaladsption.ThemeasurementsofN2adsptiondesptionFTIREDSdemonstratedthatPPLhadbeenadsbedintothechannelsoftheMPS.Hydrolysisreactionoftriacetincatalyzedbyimmobil

6、izedfreeenzymehadbeenstudied.TheoptimalreactionconditionsoffreePPLispH=7.035℃whereastheimmobilizedPPLwaspH=8.045℃.TheresultsindicatedthatimmobilizedPPLhadtheexcellentadaptabilityinwidepHtemperaturerangesofreactionmedium.

7、ComparedtofreePPLtheimmobilizedPPLsamplespossessbetterthermalstabilitywhichstillretainabout40%oftheinitialactivityafterbeingreusedsixtimes.ThemesopousmaterialswithdifferentdistributionsofpesizenamedMSDS1.2MSDS6MSDS12MSDS

8、60werepreparedusinggelatinanionicsurfactantsodiumdodecylsulphate(SDS)asadualtemplatesodiumsilicateassilicasource.ByaddingdifferentconcentrationsofSDSthedistributionofthegeingelatincouldbechangedelectrostaticinteractionin

9、termolecularhydrogenbondwereeffectedthevolumeofgelatinchainwasfurtheradjusted.Inthiswaythedistributionofpesizeofsilicawascontrolled.ThematerialswereacterizedbyN2adsptiondesptionEDSTEM.Theresultssuggestedthatmesopoussilic

10、astillhadamphouswmholelikepestructuremeoverthemphologystructureoffourmaterialsweresimilar.PPLwasimmobilizedonMSDS1.2MSDS6MSDS12MSDS60withphysicaladsptionatoptimalimmobilizedconditions(25℃6hphosphatebufferpH=7.0).Theimmob