四種石斛抗腫瘤活性的研究.pdf

1、合肥工業(yè)大學(xué)碩士學(xué)位論文四種石斛抗腫瘤活性的研究姓名：鮑麗娟申請學(xué)位級別：碩士專業(yè)：農(nóng)產(chǎn)品加工及貯藏工程指導(dǎo)教師：羅建平20071201StudyonantitumoreffectsoffourspeciesofDendrobiumAbstractDendrobiumhuoshanense，Dendrobiumcandidum，DendrobiumnobileandDendrobiumfimbriatumareusedintraditi

2、onalChinesemedicineInordertoprotectandexploittheirresourcesreasonablytheirantitumoreffectshavecarriedoutwithtwohumancarcinomacelllines(HelaS3，HepG2)inthispaperMTTassaywasperformedtoinvestigateinvitroinhibitoryeffectofdif

3、ferentpolarsolventextractsfromDendrobiumspeciesonHelaS3andHepG2cellsHoechst33342／PIdoublestainingandagarosegelelectrophorosis(AGE)wereusedasqualitativemethodstoidentifyapoptosisTheexpressionofprotooncogenescmycWasmeasure

4、dbyRTPCRTheresultsindicatedthatwaterextracts，petroleumetherextracts，ethylacetateextracts，nbutanolextractsfromfourDendrobiumspeciescouldinhibitthegrowthofHelaS3andHepG2ceilsatdifferentlevels硼1eeffectseemedtobedose—depende

5、ntandtimedependentintherangeoftrialconcentrationsItWasfoundthatinhibitoryraterosefustanddescendedlater、析mtheprolongingofculturetimeThehighestinhibitoryrateappearedatthefourthdayTheinhibitoryeffectwasbetterwhenHelaS3cells

6、treatedbywaterextractsfromDendrobiumhuoshanense，ethylacetateextractsfromDendrobiumcandidum，waterextractsfromDendrobiumnobileandDendrobiumfimbriatum，respectivelyTheirmeanlethalconcentrations0c50)were1163m酌：11l，1418rag／m1，

7、1511mg／m_1and589mg／ml，respectivelyTheinhibitoryeffectWasbetterwhenHepG2cellstreatedbypetroleumetherextractsfromDendrobiumhuoshanenseandDendrobiumcandidum，waterextractsfromDendrobiumnobileandDendrobiumfimbriamm，respective

8、lyTheirmedianlethalconcentrations(IC50)were853rag／m1，1081mg／rnl，380mg／mlandl776rag／ha，respectivelyThemorphologicalobservationshowedthattheceilsofcontrolgroupappearedblueandthestructureofnucleuswerenormal，buttheredcellsin

9、creasedwhentreatedbyextracts，thenucleuswereroundinshapeandchromatinagglutinationwasclearresultingincelldeathDegradationofthenuclearDNAsofapoptoticcellsWasdemonstratedbyvisualizationof‘‘DNALadders’ongeleleetrophorosis72ha