TGF-β1和TGF-β1型受體蛋白及其mRNA在胃癌中的表達及臨床意義研究.pdf

1、青島大學碩士學位論文TGFβ1和TGFβ1型受體蛋白及其mRNA在胃癌中的表達及臨床意義研究姓名：韓慧申請學位級別：碩士專業(yè)：病理生理學指導教師：徐珞20080601AbstractObjective：Theaimofthisstudytoevaluatetherelationshipbetweentheexpressiontransforminggrowthfactor一131(TGF131)proteinandtransformin

2、ggrowthfactorpItypereceptor(TGFpR1)inthepathogenesisanddevelopmentofgastriccarcinomatoprovidetheoreticalbasisforevaluationofgastriccancer’S，therapyandprognosisofgastriccancerMethods：50casesofgastriccarcinoma，19casesofatr

3、ophicgastritisand18casesofnormalgastricmucosasampleswerecollectedTheTGF一131proteinandTGF—pR1weredetectedbyusingimmunohistochemistryandrealtimePeRThedataWasanalysizedbysoftwareSPSSl00戈testandspearmancorrelationwereusedtoc

4、omparedifferencebetweengroupsPvalue001WaSconsideredasstatisticallysignificantResults：TGF131proteinandTGFpR1werestainedmailyinthecytoplasmofcellsandoccasionallyevidentinfibroblastsandsmoothmusclecellsNormalepithelialcells

5、werehomogeneouslystainedbyTGF131andTGFpR1，whereasthepositiveturnorcellswereheterogeneouslydistributed、析也ingaStriccarcinomanormalgaStricrnucosaandgastriccarcinomaThereWaSasignificantlymuchhigherpositiverateofTGFp1andTGF—B

6、R1usingimmunohistochemistryingastriccarcinoma(80O％and750％)thanthatinnormalgastricmucosa(333％and278％)andatrophicgastritis(368％and368％)(尸001)ThelowerthedifferentiationofgaStriccarcinomatissue，thehighertheexpressionofTGF—pl

7、proteinandTGFpR1(r=3558，P001)FurthermoreourdatashowsthattheexpressionsofTGF一131andTGF—pR1mRNAweresignificantlyincreaSedingastriccarcinomatissuecomparingwithnormalgastricmucosaandatrophicgastritis(尸O01)Conclusion：TGF—131a

8、ndTGF13R1areinvolvedinthegastriccarcinomacellsproliferationandmalignanttransformationTheymaybeusefultoevaluatethebiologicalbehaviorsandpredicttheprognosisofgastriccarcinomaPostgraduatestudent：HuiHan(pathophysiology)Direc