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1、山東輕工業(yè)學(xué)院碩士學(xué)位論文高富集生物活性硒啤酒酵母的研究姓名:孫海云申請(qǐng)學(xué)位級(jí)別:碩士專業(yè):發(fā)酵工程指導(dǎo)教師:于同立20080610山東輕T業(yè)學(xué)院碩lj學(xué)位論文ABSTRACTSeleniumiSoneofthenecessaryelementstomaintainthenormallireactivityofhuman’Sbody。Theuseofselenizedyeastasenrichedseleniumsupplementsi

2、nhumannutritionhasbecomeatopicofincreasinginterestoverthelastdecadeThissubjectusedthebeeryeastoflaboratoryastheoriginalstrains,strainswerefilteredfortheresistancetotheSeleniumandoptimizedthecultureconditionsoftheSerich,a

3、ndbyaseriesoftrainingmethodsmadetheabilityofseleniumrichedhadagreatincrease,suchashandledofthecellwallusingacomplexenzyme(snail—enzyme),ultrasoundtreatmentinthecellmembraneandincreasedpressureinthecultureprocess,anditlai

4、dagoodfoundationforthefurtherappliedtoindustrialproduction。ThethreebrewersyeaststrainswerefilteredfortheresistancetotheSeleniumwiththemethodofflat—panelcolonycountingThroughdetectedtheirbiomassandseleniumintakerateinthem

5、ediumcontainingthesodiumselenitesolution,andthestrain362khadhigherbiomassandresistancetothesodiumseleniteTheexperimentalconditionsofthemethodfordetectingtheselo玨i疆l秘————≮hereductionofmethyleneblueWasimprovedthroughthesin

6、glefactorandorthogonalexperiment,andtheresultshowed:Asthelightwasweak,thereactiontemperaturewas32“CandformaldehydeaddedquantitywaslmL,thereactionspeedWasappropriatetotime,lineargood,andsensitivitywashighTheSe—richedcultu

7、reconditionsforthestrain362kwereoptimizedthroughsinglefactorandorthogonalexperimentsandconfirmeditsoptimalcultureconditions:thetemperaturewas28℃,theconcentrationofsodiumseleniteaddedwasbatchto20;tg/mL,thevaccinationwas10

8、%(v/v),runningspeedwas200r/min,andculturedfor48h,the362k’Sgrowthconditions,biomassandSe—enrichmentcapacitywasbest。Inthisoptimalconditions,seleniumyeastbiomass(dryweight)couldupto84869/L,thetotalseleniumcontentreached7064

9、2399/gdrycell,includingorganicseleniumwas6429161tg/gdrycell,theconversionratereached9l。01percentItcouldbeshowedthatthebeeryeast’Sabilityoftransformingtheinorganicseleniumtoorganicselinumwasstrongthroughtheoptimizationofc

10、ultureconditions,ithadreachedmorethan80percentSotheseleniumintakebecameacriticalfactorofseleniumenrichedrateInthisissueused搬ethreemethodstoincreasetheintakeofseleniumfollowing:thetreatmentcellwallwithcompoundenzyme,ultra

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