中國(guó)6個(gè)地方水牛類群mtDNA遺傳多樣性與分子系統(tǒng)進(jìn)化研究.pdf

1、111329S2諺西娃農(nóng)；l=、辱旋太學(xué)2007屆≯嘍碩j^學(xué)住研究生學(xué)位f畢止)倉(cāng)丈=!=l國(guó)6個(gè)繾方水牛類群mIDNA選傳多樣毪與分子系統(tǒng)邂詫研究學(xué)科業(yè)齲岔方向研究生如導(dǎo)教焉完成畔閻■岱I垡jl互摶≥蟹盟一三扭蘊(yùn)盛生葚煎直鹽一一雎慢一煎烈腳—￡蛻盤PL盅丑—援——0蝕型生L‘!L一羔一黛№STUDYONGENETICDIVERSlTYOFMTDNAANDMoLECULARPHYLOGENYEVOLUTIONINSIXCHlNESEI

2、NDIGENoUSBUFFALOTYPESPostgraduate：ZhangWeiTutor：AssociateProfiLeiChuzhaoProfChenHong(CollegeofAnimalScienceandTechnologyNorthwestA&FUniversityYanglingShaanxi712100PRChina、ABSTRACTChinaisoneofthecountriesthathavetheriches

3、tswampbuffaloresourcesintheworldThenativebuffalotypesinChinaarenotonlyexpensivenaturalresourcesbutalsoeconomicresourceswithhi曲valuesBecauseoflackingcomprehensiveandsystematicknowledgeonthegeneticdiversityandcharacteriSti

4、csofindigenousbuffalotypes，weonlyusedthenativebreedsasthefemaleparentsandtheforeignbreedsasthemaleparentstohybridwhichhasledtothedeclineofthegeneticdiversityofbuffalograduallyandeventhedistinctofsomegeneticresourceofbu髓1

5、0soonSoitishelpfultOtheconservationandutilizationofanimalbreedresourcest0studythebuffalos’geneticdiversity髓rougIanalyzingthemtDNAD—loopof54samplesfrom6ChineseindigenousbuffalotypeswiththepublishedinGenBankabouttheriverbu

6、ffaloandtheswampbuffalomtDNADloopinBrazilandItalywestudiedthematernaloriginofthebuffalos，thephylogeneticrelationsbetweengeneticvarietiesanddiversityandgottheseresultsasfollows123hyplotypesweredetectedin54Chinesebuffalosa

7、mplesTheaveragenucleotidecontentare：2758％forZ33”％forA15，30％forGand2637％forCAverage(ADcontentis5833％and4167％for(GC)，ATtheformerisapparentlyhigherthanthelatteritissuggestingthatthebasebiaswhichisinproportiontomammalianmito

8、chondrialDNAnucleotidecomposition254sitesarepolymorphicin23hyplotyposwith612％of915bpDloopregion51ofthesesitesaretransitions，3ofthemaretransvertions，2ofthesesiteshavebothtransitionsandtransvertions，3Thelengthofthecomplete

9、mtDNADloopsequencesvariedfrom910bpt0920bpin54individualsof6ChinesebuffalotypesThehaplotypediversityandnucleotidediversitywereO533～0953and00011～00204respectivelyindicatingabundantgeneticdiversityinChinesebuffalopopulation