可降解聚酯材料行鼓膜穿孔修復(fù)的實驗研究.pdf

1、復(fù)旦大學(xué)碩士學(xué)位論文可降解聚酯材料行鼓膜穿孔修復(fù)的實驗研究姓名：崔西棟申請學(xué)位級別：碩士專業(yè)：耳鼻咽喉科學(xué)指導(dǎo)教師：遲放魯20040510復(fù)旦大學(xué)碩士畢業(yè)論文可降解聚配材料行鼓膜穿孔修復(fù)的實驗研究關(guān)鍵詞大鼠鼓膜穿孔鼓膜修補生物材料聚酷ExperimentalStudyofTympanicMembraneRepairWithaDegradablepolyesterAbstractO川ectivesChronictympanicmembra

2、neperforations，whichusuallycausehearinglossandotitismediaareacommonprobleminotolaryngologyandsurgeryneededtepairetheperforationisnotseldom.Althoughsurgicaltympanoplastyusingtemporalisfasciaetchasproventobehighlysuccessfu

3、lthesematerialsarenotwithouttheirownlimitations.Theaimofthisstudyistoevaluatetheefectivenessofonebiodegradablepolyester(PLGA85:15)asagraftmaterialintympanoplastybycomparisontosutologousfasciainachronictympanicmembraneper

4、forationanimalmodelandtrytoprovideasafe，costefectivealternativetotraditionalmaterialscurrentlyusedforpatchtympanoplasty.MethodsProspectivestudyusingadultSDratstwostepsareneededtocompletetheexperiment.ThefirststepSubtotal

5、tympanicmembraneperforationsintheparstensawerecreatedbilaterallybymechanicalcombinedwithchemicaldamagein35adultrats60earsinexperimentalgrouptreatedwith0.2mgmlofmitomycinCand5mgmlofhydrocortisoneontheperforationrimsthelef

6、tcontrol10earsreceivedsterilephysiologicalsaline.Theresidualperforationsareexaminedweeklyaftertheoperationfor6to8weeksandpersistentperforationswithouthealingtendencyforconsecutivetwoweeksareassuccessfulmodels.Thesecondst

7、ep，47stableperforationsweredividedintotheexperimental(38ears)andblankcontrol(9ears)groups.1ntheexperimentalgroupeachanimalservedasitsowncontrol勿undergoingpolyestertympanoplastyintherightearandfasciacontroltympanoplastyin

8、theotherear.8weekslaterThetympanicmembraneswereexaminedandthenharvestedforhistopathologicalanalysis.Perforationclosureratesgrossandhistologicalanalyseswerecomparedamongthepolyesterfasciaandblankgroupsandtheresultsweresta