抗感冒藥物的HPLC分析及GC硫安培電化學(xué)檢測器的初步研究.pdf

1、河北大學(xué)碩士學(xué)位論文抗感冒藥物的HPLC分析及GC硫安培電化學(xué)檢測器的初步研究姓名：劉國瑞申請學(xué)位級別：碩士專業(yè)：物理化學(xué)指導(dǎo)教師：孫素芳20070601，Abstract鼉!曼鼉詈!!蘭=皇鼉!=!竺!!蘭!!!!!!皇!!!!苧苧苧曼!!!!苧皇!苧苧苧!竺詈!!鼉皇!詈苧=皇!!皇!苧!詈!!!!苧皇!皇Z2ZZ_。苧!皇!!!苧皇詈!AbstractHPLCisafastandaccurateanalyticaltechniqu

2、edevelopedinthelateof60’Sandearlystageof70’SofnineteenthcenturyAtpresent，HPLCmethodhasbeenuniversallyappliedinpharmaceuticalanalysis，foodscience，biologicalscience，medicalscience，ecologicalandenvironrnentalscienceduetoits

3、highseparationefficiencysensitivityandgoodreproducibilityAHPLCmethodwasfirstlyestablishedforthedeterminationofchlorphenaminemaleate(CPM)，andproblemaboutwhichpeakshouldbeusedforthedeterminationofCPMwasdiscussedThechromato

4、graphicconditionswereasfollows：PhenomenexCls(250minx60mm，5腳n)chromatographiccolumnwasemployed，Themobilephaseconsistedofmethan01005molL?！畃otassiumdihydrogenphosphatesolution(50：50；v／v)withflowrateseRedat10mLmin1，anddetect

5、ivewavelength264nlnwasselectedTheresultsshowedthatthecalibrationcurveforCPMWaslinearintherangeof20—400腭mL’1；thecorrelationcoefficient(r)Was09998Theaveragerecovery(n=9)ofCPMWas992％，theRSD(n29)was071％Asseenfromabove，themet

6、hodWassimpleandsatisfactorymightbedevelopedforthedeterminationofCPM。AnopinionofourownwasproposedaboutwhichpeakshouldbeusedfordeterminationofCPM，theoriginoftheproblemWaspointedoutandsomemeasurementsofavoidingtheproblemwas

7、suggestedforthedeterminationofCPMAsimpleandrapidHPLCmethodusingphenacetin(PHN)asinternalstandardhasbeenalsodevelopedforsimultaneousdeterminationofacetaminophen(AMP)，caffeine(CAF)，andCPMintheproductcompoundparacetamolandc

8、hlorphenaminemaleategranulesSeparationandquantitationwereachievedona250mm46mill，5岫particle，ClscolumnThemobilephasewasmethanol005mol‘L～aqueousKH2P04solution，45：55(v／v)，containingO1％triethylamineandadjustedtopH36byaddition