瓜類細(xì)菌性果腐病病原菌鑒定和生物學(xué)特性的研究.pdf

1、福建農(nóng)林大學(xué)碩士學(xué)位論文瓜類細(xì)菌性果腐病病原菌鑒定和生物學(xué)特性的研究姓名：李鵬申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：植物病理學(xué)指導(dǎo)教師：唐樂(lè)塵古勤生20060401福建農(nóng)林大學(xué)碩士學(xué)位論文ABSTRACTThestudyhascarriedonseveralresearchtocucurbitbacterialfruitblotchthroughmodemmolecularidentificationandtraditionalbiologicalm

2、ethodsTwostrainswereisolatedfromthediseasedplantwhichwassuspectedtobethecucurbitbacterialfruitblotchthatoccuredinHenanprovinceofChinaAccordingtotheirmorphologyculturalcharacteristics，pathogenicity，hostrange，ELISAandmolec

3、ularidentification，thestrainswereidentifiedaspathogenAcidovoraxavenaesubspcitrulli(acronym：Aac)ItwasthefirstdemonstrationthatwatermolonandmelonbacterialfruitblotchinHenanwascausedbyAcidovoraxavenaesubspcitrulliTheInterge

4、nicTranscribedSpacers(ITS)regionbetween16Sand23SrDNAgeneswereamplifiedbyuniversalPCRprimersTheresultshowedthatITSsequencesofthestrainsinHenanandHainanwereupto9867％and99，55％witllthesimilarityofAmericanstrainFC520respectiv

5、elySomefactorsrelatedtothecucurbitbacterialfruitblotchwereinvestigatedinthegreenhouse，suchastimeofseedsimmersioninpathogensolution，temperatureaffectinginvasionbypathogen，thequantitiesofpathogenonseedssurfaceThetimeofseed

6、simmersioninpathogenshowedtheincidenceofdiseasewashighestwhenseedssoakedin30minandlhwithdiseaseincidencesupto95％ItprovedthatthepathogenstickedtothesurfaceofseedsquickilyThetempretureexperimentshowedthathightemperaturewas

7、favorabletotheemergenceofthisdiseaseand35“CwasthemostsuitableforitTheamountofthepathogenesonwatermelonseedssurfaceshowedthattheconcentrationbetween104CFU／ml～10sCFU／mlcouldcausediseaSetlleincidenceofdiseasecametotheculmin

8、ationat108CFU，Inl，whichindicatesthatpathogenhasanextremelystrongaggressiveness’InoculationbysprayingdifferentisolatesofpathogensuspensiononwatermelonfemaleflowersandyoungfruitswasconductedinthefieldTheresultshowedthatall

9、fruitsproducedfrominoculatedflowersdidnotdisplaythesymptomInoculatedyoungfruitsshowedsymptomafterthreeweeksThefruitshadtwokindsofsymptoms：restrictedlesionandexpandinglesionThispathogeninvadetheseedsbothatfiowefingstagean

10、dfruit—settingstageFloweringstagewastheimportantperiodforinfectionTheseedsfromthefruitswithrestrictedlesionhadthepathogenTheincidenceofdiseaseonseedlingshastheremarkabledifferencewitlthevariousstrainsorthedifferentinocul