自體熒光法診斷早期胃腸癌的實(shí)用化研究.pdf

1、天津大學(xué)碩士學(xué)位論文自體熒光法診斷早期胃腸癌的實(shí)用化研究姓名：徐智申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：光學(xué)工程指導(dǎo)教師：郁道銀2001.1.1AbstractWithoutaddedphotoconductivesubstance，nativefluorescenceinducedbylaserreflectsthecharacteristicsoftissueNativefluorescencediagnosisiSamethod，whichdi

2、fferentiatesnormaltissuefromthediseasedbynativefluorescenceItwouldbeappliedwidelybecauseithasthefeatures，suchasreal—time、non—invasive、correctandobjectiveetcThispaperintroducesthedetailedresearchonhowtoapplynativefluoresc

3、encetechniquetodiagnoseearlygastrointestinalcancerinclinicuseThecharacteristicofnativefluorescenceisrelevanttoexcitedwavelengthInthispaper，Nitrogenlaserwithexcitedwavelength337nmisusedasinducedlightsourceAfteranalyzingga

4、strointestinalnativefluorescencespectradetectedbyOMA，wefindthatitjssimilarbetweennormalandcanceroustissuefluorescencespectmmwhilemainpeakofcancerousfluorescencespectrashiftstowardtheinfraredwavebandSpectraaredividedintot

5、wopartsof300460nmand460660nmatthedivisionwavelength460nmInthesetwoparts，theenergeticratio(W300460／w460—660)ofnormaltissueishigherthanthatofcanceroustissueandtotalfluorescenceenergyofnormaltissueisalsostrongerthanthatofca

6、nceroustissueTheaccuracygoesuptO90％，whenweadoptthetwoabovementionedfluorescenceenergeticcharacteristicsasthecriteriatodiagnosecancerBasedonthefluorescenceenergeticcharacteristics，twoPMTs(PhotomultiplierTube)areusedtOdete

7、ctenergyoffluorescenceinthewavebandfrom460through660nm(mainpeak)andfrom300through460nm(minorpeak)，respectivelyInthispaper，wecompletethefluorescencespectroscope、electromagneticshieldandmechanicallyhermeticaldevicesuitable

8、totheapplicationofPMTOnespecialopticalfiberbundleconsistedofninefibersisdesignedtotransmitlight，amongwhichexcitedlaserisguidedinoflefiberintotissueandtheothersinchargeoftransmittingfluorescencesignaloutsideAdditionally，o

9、nelensisutilizedtocoupleopticalfiberandthetwoPMTsFromtheresultsof15patientsdetectedbytwoPMTs’systemjnvivoandvitroitisshowedthatratiooffluorescenceenergyisnotaffectedbycongestion、bloodingandthedistancebetweenopticalfiberd

10、etectorandtissueButfibrillatetissuehassimilarenergyratiotocanceroustissue，SOitcouldbewronglytakenascanceroustissueonlybyenergyratioTherefore，weselectenergyofminorpeaktomakeupenergyratiocriterionbecauseitiseffectivetodist