靜電自組裝糖胺聚糖抗體血管支架涂層的研究.pdf

1、大連理工大學(xué)碩士學(xué)位論文靜電自組裝糖胺聚糖抗體血管支架涂層的研究姓名：隋景晗申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：藥物工程指導(dǎo)教師：張世軒20080603靜電自組裝糖胺聚糖抗體血管支架涂層的研究PreparationandEvaluationofEndovascularStentBasedonHyaluronanandChitosanMultilayersCoatingLoadingwithAntibodyAbstractIntravasculars

2、tentinghasbecomeoneofthemosteffectivetherapymethodsfortreatmentofcoronaryarteryangiemphraxisdiseasesBaremetalstentscancauseinstentrestenosisDrugelutingstents(DES)havedramaticallyreducedtheincidenceofinstentrestenosisandt

3、heincidenceofadverseeventsBut，DESwouldleadhypersensitivityreactions，inflammatoryreactions，vascularintimalhyperplasiaandlatestentthrombosisTheemergenceofEndothelialProgenitorCell(EPCs)capturetechnologyrepresentsthenextgen

4、erationstentsapproachtocontrollingstentthrombosisandrestenosisInthisresearch，HyaluronanandChitosanelectrostaticselfassemblymultilayerscoatingendovascularstentsloadingwithCD34orCD133antibodywerefirstpreparedObservingthesu

5、rfaceofbasiscoatingbyatomicmechanicsmicroscopetherewasuniformlydenseislandarrangement。硒ebioactivematrixcoatingseffectivelyimprovedhemocompatibilityofthemetalstentsurfacebyplateletadhesionexperimentbyscanningelectronmicro

6、scopyThefeasibilityofaccelerateendothelializationwasevaluatedbyscanningelectronmicroscopyandimmunofluorescencenleantibodystentscancaptureEPCsrapidlyinfreshhumanperipheralbloodinvitroAfterimplantingCD34orCDl33coatedstents

7、inrabbitvesselEPCsintheperipheralbloodCanbequicklyandspecificitycapturedbyCD34orCDl33monoclonalantibodyonthestentsurfacedifferentiatedintovascularendothelialcellsbyfluorescenceimmunohistochemistry48hoursIaterabout85％ofst

8、entsurfacewascoveredwithmonolayerv2LscdarendothelialcellsCD34orCDl33antibodystentswouldacceleratetepairinjuredvesselcausedbystentexpanding，whichwouldbeamorenaturalstrategytopreventrestenosisandthrombosisTheglycosaminogly