17164.幾種新型熒光探針的細胞毒性研究及其在成像分析中的應用

1、分類號學校代碼10542密級學號2Q120212104l幾種新型熒光探針的細胞毒性研究及其在成像分析中的應用Thecytotoxicityofseveralfluorescentprobesanditsapplicationsinimaging研究生姓名彌娜修指導教師姓名、職稱張友玉教授學科專業(yè)藥物分析學研究方向生化分析與生物傳感湖南師范大學學位評定委員會辦公室二零一五年四月ABSTRACTFluorescenceimagingtech

2、nologyhasgoodselectivityandhighsensitivityInrecentyears，ithasattractedmoreandmoreattentionandhasbeenwidelyusedinvariousfieldsofbiological，environmental，medicalandothersBecauseofthefluorescentstabilityandlowtoxicity，molec

3、ularfluorescentprobesandfluorescentnano—materialsareusedforbiologicalimagingTherefore，wesynthesizedseveralfluorescentprobestostudytheirapplicationsinbiologicalanalysisThemain’contentsweresummarizedasfollows：(1)Inthiswork

4、，anovelfluorescentprobewithhighselectivityhasbeendevelopedforlivecellimagingofcysteine(Cys)TheprobeisdemonstratedtohaveexcellentbiocompatibilityhighintracellulardeliveryefficiencyandstabilityCellcytotoxicityandbioimaging

5、studiesrevealedthatprobewascellpermeableandcouldbeusedtodetectCysinlivingcellwithlowcytotoxicity(2)Anovelfluorescentprobewithhighselectivityhasbeendevelopedforlivecellimagingofcysteine(Cys)，homocysteine(Hcy)andGlutathion

6、e(GSH)TheprobeisdemonstratedtohaveexcellentbiocompatibilityhighintracellulardeliveryefficiencyandstabilityforobservationsCellcytotoxicityandbio—imagingstudiesrevealedthatprobewascellpermeableandcouldbeusedtodetectCyswith