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1、寧夏大學(xué)碩士學(xué)位論文伏毛鐵棒錘營(yíng)養(yǎng)器官結(jié)構(gòu)發(fā)育與生物堿積累變化規(guī)律的研究姓名:顧潔申請(qǐng)學(xué)位級(jí)別:碩士專(zhuān)業(yè):植物學(xué)指導(dǎo)教師:王俊20100301AbstractAconitumflavumHand—Mazzbelongstanales,RanunculaceaeSubfamintheplantsystemclassification,Astraditionalnationalitymedicinesformanykindsofcurati
2、veeffectandthecommonlyusedChinesemedicineitisallimportantmedicinalplant,whosebiologicalactivitycomponentsaremainlyalkaloidsInthisstudythedevelopmentandstructureofroot,stemandleafandthedynamicchangesoftotalalkaloidsinthem
3、duringtheirdevelopmentweresystematicallyinvestigatedbyusinganatomyhistochemistryandphytochemistrmethodsWeusedParaffmSectionMethodsinordertostudytheAconitumylavumHandMazz’sdevelopmentanddissectionstructureofvegetativeorga
4、n111eresultsofthemicroscopephotographshowedthatthechildrootandthefemaleroothavethe$arflenewbornandsecondarystructure’soccurrenceandconsistentforms,whoweremadeofsecondarycortex,marrowvasculartissueTheprimaryxylemkeel’snum
5、berchangeisverybiganditmayfrom2prototypesto9prototypesneoverstrikeinggrowthisattributedtothecambiumcellandthemeristemcell’sactivityThisindicatesthatthechildrootandthefemalerootisrelatedtoheredity,thechildroot100kedthatth
6、emajorpartastherootstructurefromthecTosssection,fromthelongitudinalsection,itstilltohavetheterminalbudabovewhichwaslivingsomelamellaeLookedfromtheaxialbuddifferentiationsearlytime,itsshapeissimilarasembryonelatergrowthju
7、stisthisrootbaseexceptionallyexpandsandmayalsocallitthetuberMateriallythechildrootmayregardasisAconitumflavumHandMazzsrenewalsproutInfactThiskindofthechildroot’sreproductionwayisonenutritionreproductionwhichreproducesbyt
8、heundergroundrhizomeThereforethechildrootsessenceisalsothenutritionvegetativeformwhichsurvivesthewinterThevegetativeorganoarriesonthehistochemistrylocalizationtoFuMaotietoindicatethatafterthebiologicaltotalalkalimeetsthe
9、iodizatebismithpotassium,IKIandthephosphowolframicacidsolution,thedistinctionformsthemassivereddishoranges,thereddishbrownaswellasthefaintyellowgranulatedprecipitationrapidlyThealkaloidofAconitum肋ⅧmHandMazzexistesinthero
10、otandstem1heresultsofhistochemistryinthevegetativeorgansshowedthatthealkaloidwagmainlydistributedintinthefemalerootandthechildrootinthemarrowparenchymacell,inthePhloemparenchymacellaswellastheraycell,thealkaloidmainlydis
11、tributesinstemscorticalcells,tiebetweentheparenchymacellandthemarrowcellinthestemAsaresult,thealkaloidwasmainlydistributedintheparenchymacellsItcouldbesuggestedthatthehighertheratioofpartsoutsidesthevascularcambiumtopart
12、sinsidesthevascularcambiumwasthemorethealkaloiditcontainedTherefore,thisratiocouldbeusedasallanatomicalcriteri∞toestimatethealkaloidcontentOnlytheehildroottobeusedasmedicineintraditionthatisonercsouro晦wasteandwesuggested
13、thatthefemalerootandthestemshouldbeusedwellinpresentsrawmaterialformedicineprocessingneresearchinvolvesinthetotalalkaloidcontentof也eAconitumaavumHandMazzwascarriedoutinordertoevaluatethequalityoftheherbsandmanipulatetopl
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