轉基因小麥的種質鑒定及其RAPD分子驗證.pdf

1、南開大學碩士學位論文轉基因小麥的種質鑒定及其RAPD分子驗證姓名：劉君申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：呂憲禹2002.5.1AbstractThetotalDNAextractedfromthewildsoybeanwasintroducedintotheembryosofcommonwinterwheatthroughapollentubechannelafterselfpollinationAgreatrang

2、eofvariationsappearedintheprogenyoftherecipient，fromwhichsomenewwheatlineswithhighyieldandgoodqualitywereselectedThisworkisveryimportantforcropbreedingAfteranalyzingtheproteincontent，aminoacidingredient，globulincontent，a

3、ndagronomiccharacteristics，wechooseanewwheatlinecalledNo16withhighyield，highproteinandhighlysine，thenweuseRAPDskilltoidentifythenewwheatline，andtheresultsareasfollows：①Theaverageproteincontentincreasedabout58％in38wheatli

4、nesofthesecondgenerationand14wheatlinesincreasedover10％Amongthe7wheatlinesofthethirdgeneration，only2wheatlinesincreasedtheproteincontent，andanewwheatlinenamedNo16increasedabout1072％@Theaverageglobulincontentof38newwheatl

5、inesincreasedabout84％and10wheatlinesincreasedover10％Byelectrophoresisanalysis，theproteinofNo13andNo16wheatlinesappearedtobedifferentcomparedwiththecontr01@ThetotalaminoacidcontentofNo16wheatlineincreasedabout2488％，andthe

6、No13wheatlineincreasedabout1427％④Byfieldobservationofagronomiccharacteristic，thenewwheatlinesappearedgreatvariationsinmanyaspectscomparedwiththecommonwheat，suchastheheight，theareaoftheleaves，therateofphotosynthesis，andth

7、eweight⑤RAPD(randomamplifiedpolymorphicDNA)analysisrevealedthatNo16wheatlineDNApolymorphismdisplayedinIprimerfrom25primerstestedTherefore，theNo16wheatlinewithhighyield，highproteinandhighlysinewasresultedfromthewildsoybea