白三葉與紅三葉體細(xì)胞胚胎途徑建立再生體系的研究.pdf

1、甘肅農(nóng)業(yè)大學(xué)碩士學(xué)位論文白三葉與紅三葉體細(xì)胞胚胎途徑建立再生體系的研究姓名：王曉春申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：草業(yè)科學(xué)指導(dǎo)教師：師尚禮梁慧敏20090601SummaryTrifliumListhemostimportantforagecropsplantandtuffgrassThetissuecultureregenerationsystemwillsetngoodbasefortransformandtheapplicationofo

2、thertechnologiesinthefutureTheexperimentalstudiedontheprocessoftwocloverembryogeniccallus’inducingandembryoids’differentiatingandregenerations’estalishing，differentexplant，differentcombinationsandconcentrationsofhormones

3、andothersconditionseffectedtoestablishregenerationsystemandgotacompletesetoftissueregenerationsystemThematerialswereTrifoliumpratenseandfourvarietiesofTr／fot／umrepens，KoalaPersistentLadinoandPalestineThecotyledonupsideof

4、hypocotyl，downsideofhypocotylandleaveswereusedasexplantsinvitroStudiedtheeffectsofdifferentexplantsanddifferenttreatmentsconcentrated2，4Dwithlightconcentration6BAtotheembryoniccallusinductionandembryoiddifferentiationint

5、hemodifiedSHbasicmediaAndthesuitableinducingtimeoftrefoildifferentexplantsWassearchedTheresultsshowedthattheinductivityofembryogeniccallioftheupsideofhypocotylandco鑼ledonwereallhigherNeverthelessthedownsideofhypocotylWas

6、lowerHi曲concentrationsof2，4DcallpromotetheratiosofinductionsomaticembryosThebestmediuminducingembryoniccallusWasmodifiedSH10mg。L12，4一DO2mg’L～6BAInthiscombination，theresultsofembryogeniccallusinductionofreddoverandPalesti

7、ne’Supsideofhypoeotylwerebesttheratiosofthosetwocloversembryogeniccallusinductionreached885％and579％Andredclover’embryogeniccallusinductionrateswerehigherthanfourvarietiesofWhitecloverbecausewhiteclover’browningWasmoreser

8、iousthantheredcloversignificantlybutfourvarietieswhiteclovers’embryoidsdifferentiationrateswerehigherthanthereddoverindifferentiationmediaMSOwhitouthormones，andtheembyogeniccallushadawelldifferentiatedindifferentiationme

9、diaMSOTheratiosofembyoiddi婦FerentiationofPersistent，KoalaLadino，Palestineandredcloverwere453％432％457％，337％，respectively叨1ebestsubculturemediuminducingcalluswasmodifiedSH5mg’L～24一D05mg’L16BA耶1ebestsuitableinducingdaysofhy

10、pocotylwas60q55dThatofcotyledonwas70～75dDuringtheinductionofreculturalembryogeniccallusesreducingtheconcentrationofthe2，4—DproperlycouldpromotetherateofembryogeniccallusinductionandreducethewhiteclovercallusbrownThecycle