凝膠柱色譜(gel column choromatography)英文ppt

1、Experiment Methods,Massey University,Gel column chromatography,Separation Science,Content,DefinitionWhen to useMechanismAdvantages & disadvantagesAn example,Definition of gel column chromatography,Gel column chr

2、omatography is a kind of separation method that depends on the principle of different sizes of molecules can be separated by the porous polymer bead, small molecules enter the bead while large molecules can't enter

3、the bead, and medium-size molecules partially enter the bead.,When to use?,Separation in Chemistry Analytical Chemistry PhytoChemistry

4、 Natural Medicinal Chemistry Biochemistry Other fields,Mechanism,Stationary phase: The phase that is stationary and can separate and preserve the research sample

5、 in chromatography. Mobile phase: The substances that carry the analytes and move forward through the stationary phase in chromatography.,,,Advantages & disadvantages,Advantages: 1. Simple

6、 2. Good separation effect towards polymeric substances 3. Maintain the biological properties

7、 4. Estimated molecular weight of the biological macromolecules,Advantages & disadvantages,Disadvantages: 1. Time consuming

8、 2. Need to be taken care of carefully 3

9、. Preliminary separation,An example,Small molecules,Large molecules,,,Midium-size molecules,,References,Kremmer, T. (1979). Gel chromatography : theory, methodology,ap