微流控芯片上原位聚合固相萃取樣品預處理.pdf

1、重慶大學碩士學位論文微流控芯片上原位聚合固相萃取樣品預處理姓名：張曉鳳申請學位級別：碩士專業(yè)：分析化學指導教師：徐溢20040508重慶大學碩士學位論文英文摘要ABSTRACTMiniaturizedtotalanalysissystem(btTAS)isadevelopingnewfieldofanalyticalchemistryrecently1tsfinallygoalisthatallprocessofanal舛calchem

2、istrycouldbeputintoandcarriedoutonmicroehipMostresearcherspaidmcirattentiononmicromixers，micro—reactorsanddetectionwhilesamplepretreatmentprocesswassomehowneglectedsuchasmicro—separationandconcentrationonmicro—fluidicchi

3、pInthispaper，thein—sirepolymerizedmonolithicporouspolymerwhichisthermallyinitiatedwithinaglassmicro—columnWasproposedtoappliedforsolidphaseextraction(SPE)andpreconcentrationIntegratingSPEmicrocolumnandmicro—fluidicchipto

4、getheracombinationmicro—fluidicchipwithSPEmicro—cohmmWasestablishedThecapabilityofSPEmicrocolumnWaSestimatedbyselectedanal)rticalmethodshalyticalprocess，includingperconcentration，mixingreactionanddetection，wererealizedon

5、thecombinationmicro—fluidicchipat】astInthispaper，thefactorsoftemperature，oxygenfreeconditionandpolymerizationreactiontimewereinterpretedEffectsoftheamolmtofeachmonomerontheSPEmicrocolumnpropertieswerediscussedSPEmicro—co

6、lumnwimanionexchangesorbentwaspreparedinsituatglasscapillaryThestructureimageofpolymerswereobservedbymicroscopyandscanningelectronmicrograph(SEM)PomgenicsolventmixtureWasofadvantagesintheprocossofformingporousstructurepo

7、lymersduringitspolymerizationAbsorptioncharateristicofconventionalSPEcolumnWasprovedbycatalytickineticspectrophotometricdeterminationofnitrite，whichWasasystemofnitritrepotassiumbmmatebromothym01buieTheresultsshownthatabs

8、orptionefficiencyoftheconventionalSPEcolumnWaS651％andenrichmentefficientis303timesSPEmicrocolumnstillwasofenrichmentcapacityieenrichmentefficientwas290timesFurthermore，enrichmenttimescouldachieve779timeswithonlinesamplet

9、reatmentbySPEmicro—columnAllenrichmentefficientforonlineprocesswereobtainedbyUVdeterminationAccordingtothedifferentretentionpropertyofL—phenylalanineandL—ArginineinpH=960solutiontheselectivityofSPEmicro—columnwasdiscusse

10、dbytheaminoacidhydroxide—LuminolsystemItwasfeasibletoseparatecomponentswithsignificantlypropertiesontheselfdesignedcombinationmicrofluidicchipForNaN02一Ⅺ一LuⅡlin01theframeworkofamicro—fluidicchipwasdesignedSamplingmode，vel