簡介：華中科技大學(xué)碩士學(xué)位論文魔芋軟腐病的爆發(fā)機(jī)制及其基于抗性誘導(dǎo)的防治研姓名季家興申請(qǐng)學(xué)位級(jí)別碩士專業(yè)生物化學(xué)與分子生物學(xué)指導(dǎo)教師楊英20110121華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文IIABSTRACTKONJACARACEAEAMPHOPHALLUSISAPERENNIALECONOMICALCROPRICHINACTIVEINGREDIENTSINCLUDINGKONJACGLUCOMANNANWHICHISINURGENTNEEDINFOODMEDICINECHEMICALINDUSTRYTHUSKONJACISWIDELYCULTIVATEDINTHESOUTHWESTOFCHINAHOWEVERASTHESOFTROTDISEASEBREAKOUTEVERYYEAREXPLOSIVELYCONTAGIOUSLYWHENTHETEMPERATURERISESDRAMATICALLYINTHESUMMERTHISDISEASEHASDEVELOPEDINTOADEVASTATINGCATASTROPHETOKONJACPRODUCTIONINTHISWKPATHOGENRESPONSIBLEFTHESOFTROTDISEASEINKONJACWASISOLATEDIDENTIFIEDTHEPHYSIOLOGICALPATHOGENICFACTSSUCHASGROWTHPATHOGENICITYWERESTUDIEDUNDERDIFFERENTTEMPERATURESTOUNDERSTTHEOUTBREAKMECHANISMOFTHISDISEASEUNDEREVALUATEDTEMPERATURES6EXTERNALLYAPPLIEDCHEMICALACTIVATSTOPLANTDEFENCESYSTEMWEREALSOSTUDIEDTOEXPLETHEIRPOTENTICALASSOFTROTRESISTENCEINDUCERTHEMAINRESULTSWERESHOWNASFOLLOWS1SOFTROTPATHOGENES09WASISOLATEDIDENTIFIEDASPECTOBACTERIUMCAROTOVUMSUBSPCAROTOVUMPCCTHROUGH16SRDNABLASTPHYSIOLOGICALTESTS2ITISSUGGESTEDTHATES09SHOWEDHIGHERGROWTHSPEEDSTONGERMOTILITYWHENTHETEMPERATUREWASEVALUATEDABOVE30OCDENATURINGGRADIENTGELELECTROPHESISDGGEREVEALEDCOMPAREDTOMAYNOVEMBERTHEPOPULATIONOFES09INTHEKONJACRHIZOSPHEREINCREASEDSIGNIFICANTLYINJULY3ITISSUGGESTEDTHATES09MACERATEDKONJACTUBERMUCHSTRONGERUNDER30OC37OCWITHTHEPATHOGENICITYTESTEXCELLULARENZYMEPRODUCTIONWASSTRICTLYREGULATEDUNDERQUUMSENSINGPECTATELYASEPOLYGALACTRONASEWASPRODUCTEDINHIGHLEVELSONLYIFTHEBACTERIAPOPULATIONMETTHETHRESHOLD4THEPOTENTIALOF6EXTERNALLYAPPLIEDCHEMICALRESISTENCEACTIVATSWERETESTEDONTHEKONJACLEAVESCULTIVATEDINTHELABPLANTSINFIELDREVEALINGTHEINFECTIONOFES09WASINHIBITEDINSALICYLICACIDSAPCOUMARICACIDPCACINNANAMICACIDCATREATEDLEAVESPLANTSCOMPAREDTOTHECONTROLMEEXCELLULARH2O2ACCUMULATIONHIGHERPEROXIDASEACTIVITYWEREOBSERVEDINSAPCACATREATEDLEAVESSUGGESTINGTHESELEAFSPRAYINGCHEMICALSCOULDTAKEEFFECTINTHEOXIDASEBURSTOFKONJACCELLSRESISTINGTHEATTACKFROMTHESOFTROTPATHOGENKEYWDSKONJACSOFTROTTEMPERATURERESISTENCE

簡介：南京農(nóng)業(yè)大學(xué)碩士學(xué)位論文黃瓜根結(jié)線蟲病的生物防治及其生態(tài)有機(jī)藥肥研制姓名楊文飛申請(qǐng)學(xué)位級(jí)別碩士專業(yè)植物保護(hù)學(xué)指導(dǎo)教師李紅梅；王偉中20110604黃瓜根結(jié)線蟲病的生物防治及其生態(tài)有機(jī)藥肥研制機(jī)載體，加入植物免疫誘抗劑、腐殖酸、無機(jī)養(yǎng)分和微量元素等，研發(fā)兼具營養(yǎng)、植保雙重功效的生態(tài)藥肥，并建立其生產(chǎn)工藝和質(zhì)量控制程序。五是研究分析了生態(tài)有機(jī)藥肥的功效，表明該藥肥具有增產(chǎn)增收、改善品質(zhì)、提高化肥的利用率、提高作物抗逆性特別是對(duì)黃瓜根結(jié)線蟲病的誘抗性及抑菌殺線蟲的功效，從而可以大量減少有毒化學(xué)農(nóng)藥的使用，提高土壤高效利用的可持續(xù)性。關(guān)鍵詞根結(jié)線蟲；生物防治；殼聚糖；水楊酸；牛心樸子、苦豆子

簡介：華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文椪柑采后蒂腐病菌生物學(xué)特性及其防治研究姓名司偉申請(qǐng)學(xué)位級(jí)別碩士專業(yè)園林植物與觀賞園藝指導(dǎo)教師程運(yùn)江201106華中農(nóng)業(yè)大學(xué)2011屆碩士學(xué)位論文ABSTRACTTHEREAREGREATECONOMIC10SSESINCI臼MSDUETOPOSMARVESTDISEASETBCON打OLMEM，T11EM勾0RM砒ODIS劬百CIDESBUTMEE彘CTOF劬百CIDESISDI觸NT舶MPA也OGENSANDTHESYSTEMICRESISTANCETOFHN百CIDESOCCMSMORE五EQUENNYINORCHARDS也ANTHEPASTSO，ALTEMATIVE，SAFE，NOT0【IC卸LPROACHESARENEEDEDTOLLSEIN也EPOSMAN，ESTSTORAGEOFCITLLLS自【LITP優(yōu)LK粗向JITWEREUSEDASMATERIALSTOISOLATEMEPATHOGEND矽腸如刀口幻彪珊括，丘OMNATILRALROTTED仔UITANDANALYSEITSBIOLO舀CALCHARACTERSACCORDINGT0TLLEPAMOGENCH撇CTERITIC，ITSCONTROLLINGMATHODW硒STUDI咄WHICHHYDROXYBIPHENYLSHADMERCM缸I【A_BLEE任ECTONCONTROLLINGD刀嘲，毒淞捃ATMES鋤ETIME，CON皿【MERCIALSTORAGEEXPE加ENTW嬲C撕EDOUTTOEVALUATETHEE丘ECTOFHYDRO【YBIPHENYLSATT11EENDOF螄DRAGE，也EROTRATEW弱CON仃ONEDLESS也ENL0％，ANDTHEREH勰NODI弼HENCEIN盤UITQUALI夠DLLRINGSTOMGETHEMAINRESULTSWEREASFONOWS1FROMMEN砷U鋤R0訛DA肌岱矗1LITS，D刀嘲彪淞缸WASS印刪ED鋤DP嘶FIEDTHEBIOLO百CALCHARACT耐STICSWEREOBSERVEDINMISSTUDYWEFOUNDMATMEC010NYFO鋤，GROWTLLRATEOFMYCEHA衄DYIELDOFC伽LIDIASI鰣F(tuán)ICANTLYV撕EDWI也DI虢RENTCULTUREMEDIAD刀口細(xì)胞船括GREWBEST，觚DMECONIDIAOFD，Z口幻跆瑚西AMOUNTEDTO908107CELLS‘N也。1INPOTATOSUCROSEAG缸，功E0PTIMLLMTEMP鋤TL玳FORMED刀口批珊西W嬲30℃LI齜OR姒HADNOTE彘CTON也EGROWMOFD玎口細(xì)昆凇西FORTLLECONIDIAOFD刀口砌尼搬括，MELEMALTEMPEMTUREWAS75℃2HYDR0XYBIPHENYLSE航CTIVELYCON仃DLLED廿LEOCCUROFD刀口幻砌S括NROU曲I11VI缸OANDINVIVOTEST，HYDROXYBIPHENYLSHADOBVIOUSE疏CTAGAILLSTD行口細(xì)厶M，婦111VI仃OEXPERIMENTSHOWEDT11AT200MG他HYDROXYBIPHENYLS訕I(yè)TED也EDRO、油OFMEPATHOGEN，ANDI11VIVOINOCULATEDEXP鰣ENTSHOWCDMAT也EROTRATEW嬲20％BYINOCULATEDWIM200N瑪／1沼HYDROXYBIPHENYLS觚D1O105SPORESINL以D門口細(xì)ZE珊旭MEREW嬲OBVIOUSDI髓RENCE五ROMCK3STORAGEE髓CTWASSTUDIEDUSING200M鯽唱HYDROXYBIPHENYLST0PONK觚HITINMISP印ERTHERESLLLTSHOWEDMATAT廿LEENDMESTORAGEMEROTRATEWAS967％，血EREWASOBVIOUSDI鼠R睨CE蠡OMCK2533％MEANTIME，也ECONTIENTOFTA，VCANDTSSWASANALYSED，TLLERESULTSHOWEDT11ATT11EREWERENODI伍ERENCE鋤ONG仃EA缸NENTSAT吐LEENDOF2

簡介：分類號(hào)S4767UDC632學(xué)校代碼10129學(xué)號(hào)2009202069向日葵菌核病生防菌的篩選鑒定及其綜合防治SCREENINGCOMPREHENSIVEPREVENTIONOFANANTAGONISTICBACTERIUMSTRAINOFSCLEROTINIASCLEROTIUMOFSUNFLOWER申請(qǐng)人李小娟學(xué)科門類農(nóng)學(xué)學(xué)科專業(yè)植物病理學(xué)研究方向植物病害生物防治指導(dǎo)教師周洪友教授論文提交日期二〇一二年五月摘要向日葵菌核病是由核盤菌SCLEROTINIASCLEROTIUMLIBDEBARY引起的一種世界性病害，其寄主范圍十分廣泛，危害嚴(yán)重。本研究對(duì)來自內(nèi)蒙古呼和浩特市、包頭市、烏蘭察布市、巴彥淖爾市、赤峰市、通遼市以及河北省等向日葵產(chǎn)區(qū)的30余個(gè)帶有植物根的土壤樣品，進(jìn)行向日葵菌核病生防菌株的分離，并對(duì)其防效進(jìn)行測定，旨在為向日葵菌核病的生物防治提供理論基礎(chǔ)和研究依據(jù)。具體結(jié)果表明1從各地區(qū)采集的30余個(gè)根際土壤樣品中，經(jīng)過分離篩選得到細(xì)菌菌株1300多株，對(duì)核盤菌有較好拮抗效果的共25株。其中菌株CF451抑菌效果最好，抑菌帶寬度為32CM。室內(nèi)離體葉片測定，防治效果為853，盆栽和大田防效分別達(dá)到了784和646。2通過細(xì)菌形態(tài)觀察和生理生化指標(biāo)測定，結(jié)合16SRDNA序列分析，將菌株CF451鑒定為蠟狀芽孢桿菌（BCEREUS）。3菌株CF451在向日葵根際定殖動(dòng)態(tài)實(shí)驗(yàn)中，發(fā)現(xiàn)菌株CF451均能在向日葵根際和根表定殖。此外，生防菌株CF451還可在向日葵體內(nèi)很好地定殖，距向日葵莖基部5CM處可以檢測到菌株CF451的存在。4經(jīng)生防細(xì)菌菌株培養(yǎng)條件測定發(fā)現(xiàn)，菌株CF451最佳培養(yǎng)條件為25℃28℃，初始PH為6左右，培養(yǎng)48H就可達(dá)最大菌量。5通過測定菌株CF451所產(chǎn)生揮發(fā)性物質(zhì)的抑菌活性，發(fā)現(xiàn)菌株CF451所產(chǎn)生的揮發(fā)性氣體對(duì)核盤菌菌絲生長和菌核形成具有一定的抑制作用，離體葉片防效達(dá)836。通過分離純化蠟狀芽孢桿菌CF451所產(chǎn)生的揮發(fā)性抑菌物質(zhì)，結(jié)果表明，揮發(fā)性物質(zhì)抑菌物質(zhì)至少包括18種揮發(fā)性物質(zhì)，這些物質(zhì)其中包括醇類、胺類、烷類、腈類和酸類物質(zhì)。對(duì)這些揮發(fā)性物質(zhì)的純品進(jìn)行抑菌活性檢測，結(jié)果發(fā)現(xiàn)，有2種物質(zhì)1METHYLDODECYLAMINE十二烷基甲胺和ACETAE2CYANO氰基乙酰胺可以抑制核盤菌菌核的形成。6通過探索向日葵菌核埋藏時(shí)間和深度對(duì)菌核干重和萌發(fā)活力的相關(guān)性的研究，發(fā)現(xiàn)隨著埋藏時(shí)間的延長和埋藏深度的增加，菌核受腐爛程度越深，凈重減輕，萌發(fā)活力逐漸喪失，并且不同地點(diǎn)結(jié)果一致。7通過模擬內(nèi)蒙古河套地區(qū)田間冬季漫灌實(shí)驗(yàn)發(fā)現(xiàn)，冬季灌水有助于菌核降解，有效降低了菌核病的發(fā)病率。關(guān)鍵詞向日葵菌核??；生物防治；蠟狀芽孢桿菌；揮發(fā)性物質(zhì)

簡介：廣西大學(xué)碩士學(xué)位論文松突圓蚧生防菌防治試驗(yàn)及其蟲生真菌的分離鑒定姓名丁波申請(qǐng)學(xué)位級(jí)別碩士專業(yè)森林培育學(xué)指導(dǎo)教師黃寶靈20100601彳“陀D6儺觸“M、木霉屬乃玉砌D如冊口、枝頂孢霉屬彳CRP朋D聆F“所、鏈格孢屬彳Z地聊口，婦、節(jié)菱孢屬彳，砌RF挖F“，，2、擬盤多毛孢屋戶協(xié)勉腸FF唧括、炭疽屬C『DZ彪幻護(hù)紀(jì)廳“聊、間座殼屬D死矽DR砌P、球殼孢科SPHAEROPSIDACEAE的尚未確定一屬種。松突圓蚧生防菌1號(hào)為球殼孢科的一屬種，2號(hào)菌為擬盤多毛孢屬尸∞勿腸FF唧括如SP垅砌口胞。關(guān)鍵詞松突圓蚧生防菌防治蟲生真菌分離鑒定B

簡介：甘肅農(nóng)業(yè)大學(xué)碩士學(xué)位論文楊木變色菌及其生物防治研究姓名楊衛(wèi)君申請(qǐng)學(xué)位級(jí)別碩士專業(yè)森林經(jīng)理指導(dǎo)教師王有科趙桂華20090601甘肅農(nóng)業(yè)大學(xué)碩士畢業(yè)論文2009屆ABSTRACTPOPLARISONEOFCHINA’SFOURMAJORTREESPECIES，BUTALSOCHINASFASTGROWINGTREESPECIES，BUTDURINGTHEPROCESSINGANDUSEINGWOULDBEASERIOUSDISCOLORATIONOFTHEWOOD，REDUCINGTHETIMBERLEVELANDTHEQUALITYOFENDPRODUCTS，ADIRECTIMPACTONTHEECONOMICBENEFITSOFPRODUCTIONTHEREFORE，BIOLOGICALCONTROLOFPOPLARSAPSTAINFUNGIISSTUDIEDTOBCTHEVERYREALECONOMICSIGNIFICANCEFIRSTLYSTUDIEDTHESAPSTAINFUNGUSTHENSELECTEDTHESTRAINB19、析T11BROADANTAGONISTICEFFECTTOSTAINGFUNGUSANDSTUDIEDTHEANTAGONISTICMECHANISMOFB19UNDERFERMENTATIONCONDITIONSTHERESULTSAREASFOLLOWS1STAINPATHOGENLASIODIPLODIATHEOBROMAE口缸GRIFFMAUBLCATLSCPOPLARSTAIN刪YBUTCURVU／A口QALUNATARELATIVELYSLOWTHEINFLUENCEOFENVIRONMENTALFACTORSONTHESTAININGFUNGUSSHOWEDTHATBOTHTWOFUNGUSARESENSITIVETOTHEEXTREMELYACIDICCONDITIONS，ESPECIALLYCLUNATAGROWEDBETTERUNDERALKALINECONDITIONSTHANSLIGHTLYACIDICCONDITIONSTHEYWERECAPABLEOFGROWINGINTHETEMPERATURERANGEOFIO。CTO35℃THEIRMAXIMUM，MINILLLUMINHIBITIONTEMPERATUREWERE40℃AND5℃FUNGUSCANGROWBESTUNDERFULLILLUMINATIONCONDITIONS2ASTRAINB19HADSTRONGERANDSTABLEANTAGONISTICEFFECTAGAINSTSTAININGFUNGUSWERESELECTEDFROMEIGHTBACTERIATHROUGHAGARPLATEEXPERIMENT3THEWOODCHIPSTHATDEALED、ⅣI廿LB19FERMENTATIONFILTRATESTILLHEALTHYTENDAYSAFTERPLACINGINTHEDISHWHICHFILLED謝MSTAININGFUNGUSHOWEVERTHECONTROLCHIPSOCCURWITLLVARYINGDEGREESOFSTAININGAWEEKAFTERTHISSHOWEDTHATB19CANEFFECTIVELYPREVENTTHEWOODDISCOLORATION4THEFERMENTEDCONDITIONSOFSTRAINB19WEREOPTIMIZEDWHENTHESTRAINCULTUREDWITLLCONDITIONSASFOLLOWCULTUREMEDIUMTEMPERATURE35“CANDCULTURETIME46HOURS，INITIALPH60，200R／RAININSHAKE，REVOLUTIONS誦M50MLMEDIUMIN250MLCONICALFLASK，THESTRONGESTANTAGONISTICEFFECTWOULDBEEXHIBITED5STRAINB19CANINHIBITMYCELIMGROWTHANDGERMINATIONOFSTAININGFUNGUSⅡ

簡介：石河子大學(xué)碩士學(xué)位論文紅花主要病害種類鑒定及其防治研究姓名劉婷婷申請(qǐng)學(xué)位級(jí)別碩士專業(yè)植物病理學(xué)指導(dǎo)教師李國英20090601ABSTRACTSAFFLOWERRUSTDISEASEISONEOFMAINDISEASESINFECTINGSAFFLOWEROBSERVATIONTHEDIFFERENTPHASESOFSAFFLOWERRUSTBYPARAFFINMETHODANDSEMSHOWSTHATTHEPATHOGENISPUCCINIACARTHAMIINXINJIANG，IT’SLIFEHISTORYHAVEFOURPHASES，THEYARESPERMAGONIA，UREDIOSPORE，TELIOSPOREANDBASIDIOSPOREPUCCINIACARTHAMIONLYHAVEONEHOST，ITLACKOFAECIOSPOREACCORDINGTOTHEOBSERVATIONOFTELIOSPOREGERMINATION，F(xiàn)INDTHATTHETELIOSPOREGERMINATIONWASTHEHIGHESTAT18‘CTHEGERMINATIONRATEONSAFFLOWERJUICEISHIGHERTHANTHATONWATERANDDISTILLEDWATERANDTHEGERMINATIONRATEOFUREDIOSPOREWASFOUNDHIGHERINWATERANDSATURATEDHUMIDITYTHEOBSERVATIONTOUREDIOSPOREGERMINATIONREVEALSTHATTHEMOSTOPTIMALGERMINATINGTEMPERATUREFORUREDIOSPOREIS22℃ANDTHEGERMINATIONWAGVERYFEWWHENTHETEMPERATUREGOESUPTO5℃AND35℃HUMIDITYHASGREATINFLUENCEONTHEUREDIOSPOREGERMINATIONWHENTHESAFFLOWERWASGROWTH，ITWASTHEDETERMINANTTHATTHEWEATHERWASRAININESS，LOWTEMPERATUREANDTHESAFFLOWERWAEEASYTOCATCHDISEASESAFFLOWERRUSTDISEASECANSPREADFARAWAYBYINFECTEDSEEDTELIOSPOREBORNEONSEEDSISTHEMAINRESOURCEOFSAFFLOWERRUSTDISEASEINTHISEXPERIMENTTHESURFACESTRUCTUREOFSEEDCOATSWASOBSERVEDANDATESTTOTHEQUANTITYOFTELIOSPOREONTHESEEDCOATWASDONETHERESULTISTHATTHEMOLEDEEPLONGITUDINALGROOVESANDVILLOUSTHESEEDCOATHAS，THELARGERTELIOSPORE’SQUANTITYISTHROUGHINDOORANDFIELDTESTSTOTHERUSTRESISTANCE，ITISCONCLUDEDTHATTHENO4NEWSAFFLOWERANDYUMINWUCIAREEASYTOGETTHISDISEASE，WHILENO2NEWSAMOWERANDNO1TAYUANCANRESISTTHEDISEASESWELLTHEREFORE，吐1EYSHOULDBEPROPERLYPLANTEDINTHOSERUSTDISEASESPLAGUEDAREASTHEEXPERIMENTSONFUNGICIDEANDSOWINGTIMESHOWTHATTHECONTROLEFFECTISBESTWHENWEUSETRIADIMEFON15％WETHENCOMEINTURNLIKEQIU，20％TRIAZOPHOSEC，ANDHAOLIKETHEOCCURRENCEOFSAFFLOWERDISEASEVARIESFROMSOWINGTIMESAFFLOWERPLANTEDINMARCHISMORELIABLETOSAFFLOWERDISEASETHANTHOSEPLANTEDINAPRILANDMAYNEDISEASESEVERITYANDDISEASEINDEXOFSAFFLOWERSOWEDONPLASTICFILMSAREEVIDENTLYLOWERTHANTHATSOWEDWITHOUTFILMSSUITABLELATETOSOWSOWWINGWIMFILMSANDSEEDDRESSINGWITHFUNGICIDEARETHEBESTMEASURESTOCONTROLSAFFLOWERRUSTROOTROTISALSOASEVEREDISEASEINFECTINGSAFFLOWERINXINJIANGAREATHROUGHTHEANALYSISANDIDENTIFICATIONOFROOTROTDISEASEINJIMUSAERANDSHIHEZIAREA，ITSHOWSTHATROOTROTISMAINLYCAUSEDBYFUSARIUMOXYSPORUMANDFUSARIUMSOLANIOBSERVATIONSOFBIOLOGICALCHARACTERISTICSSHOWTHATFUSARIUM，THEMAINCAUSEOFTHEROOTROT，CANNOTSURVIVEBELOW5“CTHEYBEGINTOGROWONCETHETEMPERATUREREACHES10“CANDGROWBESTAT25℃30℃WHENTHEGROWTHSPEEDBEGINTOREDUCEAT30。C40“CIFTHETEMPERATUREEXCEEDS40“C，ITWILLSTOPGROWINGITCANGROWWHENTHEPHVALUERANGESBETWEEN5AND11ANDGROWSLOWESTATPH5WHENTHEPHVALUERANGESBETWEEN6AND8，ITGROWSBESTTHENWITHTHEINCREASEOFPHVALUE，THEGROWTHOFMYCELIUMBEGINSTOSLOWDIFFERENTMEDIUMTESTSSHOWTHATNLEYGROW11

簡介：福建農(nóng)林大學(xué)碩士學(xué)位論文嘧磺隆防治紫莖澤蘭的效果及其對(duì)澤蘭實(shí)蠅體內(nèi)解毒酶的影響姓名王敏霞申請(qǐng)學(xué)位級(jí)別碩士專業(yè)生物防治指導(dǎo)教師吳珍泉20090401福建農(nóng)林大學(xué)碩士學(xué)位論文嘧磺隆防治紫莖澤蘭的效果及其對(duì)澤蘭實(shí)蠅體內(nèi)解毒酶的影響5澤蘭實(shí)蠅攜帶嘧磺隆防治紫莖澤蘭效果嘧磺隆對(duì)澤蘭實(shí)蠅成蟲死亡率影響不顯著，植物上直接噴施100％嘧磺隆效果最好，植株顯癥率達(dá)到100％，其次是噴施20％嘧磺隆，顯癥率達(dá)到4239％。在澤蘭實(shí)蠅不同濃度不同方法攜帶嘧磺隆防治紫莖澤蘭效果中，澤蘭實(shí)蠅攜帶100％嘧磺隆，紫莖澤蘭顯癥率達(dá)到3752％。表明嘧磺隆對(duì)澤蘭實(shí)蠅的安全性很好，并且在攜帶嘧磺隆防治紫莖澤蘭方面也有一定的效果，但是防治成本太高，是直接噴施20％嘧磺隆在紫莖澤蘭植株上成本的35倍，只能作為一種防治紫莖澤蘭的新的理論依據(jù)。關(guān)鍵詞紫莖澤蘭；澤蘭實(shí)蠅；化學(xué)防治；生物防治Ⅱ

簡介：南京林業(yè)大學(xué)碩士學(xué)位論文短葉水蜈蚣的化學(xué)防治及其環(huán)境影響研究姓名張琦申請(qǐng)學(xué)位級(jí)別碩士專業(yè)植物學(xué)指導(dǎo)教師王賢榮20090601THECHEMICALCONTROLOFTURFWEEDGREENKYLLINGAKYLLINGABREVIFOLIARORBANDSTUDYONITSINFLUENCEOFENVIRONMENTALSECURITYABSTRACTINTHISPAPER，WESTUDIEDTHEPREEMERGENCEANDPOSTEMERGENCECONTROLOFGREENKYLLINGABYPOTANDFIELDTRAILANDTHENEVALUATEDTHEENVIROMENTALIMPACTSOFTURFAFTERBEINGTREATEDBYTHECHEMICALHERBICIDEANDITCOMBINEDWITHCORNGLUTENMEALTHEMAINSTUDYRESULTSSHOWEDASFOLLOWS1WHENTHECONCENTRATIONSOFFLAZASULFURONANDOXADIAZONWERE01G／LAND04MG／L，THEPREEMERGENCECONTROLLINGEFFECTSOFGREENKYLLINGAWEREUPTO9452％AND9315％ANDALSO，WHENTHECONCENTRATIONSOFFLAZASULFURONWERE029／LAND049／L，THECONTROLLINGEFFECTSOFMATUREGREENKYLLINGAWERESEPARATELYUPTO8225％AND9677％2THEGREENKYLLINGA’SPREEMERGENCECONTROLLINGEFFECTWAS9518％，WHENBEINGTREATEDBY0O1G／LFLAZASULFURON509／MCORNGLUTENMEAL，BUTTHEPREEMERGENCECONTROLLINGE腩CTWASONLY7222％，WHENONLYBEINGTREATEDBY001G／LFLAZASULFURONCORNGLUTENMEALCOULDSTRENGTHENTHECONTROLLINGEFFECTSOFGREENKYLLINGAWHENCOMBININGITWITHFLAZASULFURONAFTERPLANTINGGREENKYLLINGA15DAYS，30DAYSAND60DAYSW1LILEFLAZASULFURONANDCORNGLUTENMEALWERECOMBINEDINTHEFIELDTOCONTROLMATUREGREENKYLLINGA，THECONTROLLINGEFFECTWAS8566％WHENAPPLIEDONLYWIM02EDLFLAZASULFURONTHOUGHAFTERCOMBINED029／LFLAZASULFURONWITH1009／M2CORNGLUTENMEAL，THECONTROLLINGEFFECTWASUPTO9874％3THECONCENTRATIONSOF0019／LAND029／LFLAZASULFURONWHICHWEREAPPLIEDTOTHESOILHADSLIGHTEFFECTSONSOILENZYMEACTIVITIESANDSOILCHEMICALPROPERTIES，WHICHREPRESENTEDTHATFLAZASULFURONWASSAFETOSOILENVIROMENT4O01LFLAZASULFURON50EDMCORNGLUTENMEALAND029／LFLAZASULFURONLOOG／M2CORNGLUTENMEALBOTHCOULDOBVIOUSLYIMPROVEDTHEVIEWINGQUALITYANDTHEGRASSPHOTOSYNTHESISOFTHETU正5THETURFSOILENZYMEACTIVITIESANDSOILCHEMICALPROPERTIESWERESIGNIFICANTLYIMPROVEDAFTERBEINGTREATEDBYO01G／LFLAZASULFURON509／MZCORNGLUTENMEALAND029／LFLAZASULFURON1009／M2CORNGLUTENMEAL6THECORRELATIONSOFSOILENZYMES，SOILCHEMICALPROPERTIES，SOILENZYMESANDSOILCHEMICALPROPERTIESWERESIGNIFICANTLYIMPROVEDAFTERBEINGTREATEDBYFLAZ嬲ULFURONCOMBING研THCORNGLUTENMEALKEYWORDSGREENKYLLINGACHEMICALHERBICIDE，CORNGLUTENMEAL，TURFQUALITY，SOILENVIRONMENT

簡介：SUPERVISORPANYONGGUIENGINEERINGBYLIUXINHUAPOSTGRADUATEPROGRAMCOLLEGEOFFOOD_HAINANUNIVERSITYACADEMICTITLEPROFESSORSIGNATUREAPPROVEDJUNE．20LOGE本人完全了解海南大學(xué)關(guān)于收集、保存、使用學(xué)位論文的規(guī)定，即學(xué)校有權(quán)保留并向國家有關(guān)部門或機(jī)構(gòu)送交論文的復(fù)印件和電子版，允許論文被查閱和借閱。本人授權(quán)海南大學(xué)可以將本學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存和匯編本學(xué)位論文。本人在導(dǎo)師指導(dǎo)下完成的論文成果，知識(shí)產(chǎn)權(quán)歸屬海南大學(xué)。保密論文在解密后遵守此規(guī)定。論文作者簽名支11翻擘日期DOFO年石月G日導(dǎo)師簽名L番毒考日期勱年／月細(xì)本人已經(jīng)認(rèn)真閱讀“CALIS高校學(xué)位論文全文數(shù)據(jù)庫發(fā)布章程”，同意將本人的學(xué)位論文提交“CALLS高校學(xué)位論文全文數(shù)據(jù)庫”中全文發(fā)布，并可按“章程”中規(guī)定享受相關(guān)權(quán)益。回童途塞埕鑾卮溢卮I旦主生；旦生I旦三生叁查。論文作者簽名支IL翻章日期A口I口年彳月G日導(dǎo)師簽名1希磁日期M，年占月彥廂

簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文東北虎源等孢球蟲蟲種鑒定及其生物學(xué)特性與藥物防治研究姓名張靖偉申請(qǐng)學(xué)位級(jí)別碩士專業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師宋銘忻20090601東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩士學(xué)位論文II能菌素相比，妥曲株利治療后的貓產(chǎn)生了較強(qiáng)的免疫力，再次感染后卵囊的排出強(qiáng)度較小。關(guān)鍵詞關(guān)鍵詞感染調(diào)查；等孢球蟲；卵囊排出規(guī)律；藥物防治；免疫耐受

簡介：西南大學(xué)碩士學(xué)位論文中國柑桔潰瘍病菌遺傳多樣性分析及其防治藥劑篩選姓名姚廷山申請(qǐng)學(xué)位級(jí)別碩士專業(yè)植物病理學(xué)指導(dǎo)教師周常勇20090501兩南大學(xué)碩士學(xué)位論文|LIE抽提效果最好，微波法最適用于引物XCF／XCR檢測，微量抽提法及SDS適用于引物JYF5／JYR5檢測。4．比較了引物XCF／XCR和JYF5／JYR5的靈敏度，結(jié)果表明，引物XCFⅨCR的靈敏度可以達(dá)到2．110D4G／ML以上，優(yōu)于引物JYFS／JYR5的靈敏度21G／ML。5．對(duì)來自9個(gè)省區(qū)的病菌DNA進(jìn)行REP．PCR分析，結(jié)果表明引物GTG5、ITS、REP的擴(kuò)增效果不理想，引物BOX和ERIC擴(kuò)增的多態(tài)性譜帶較多，而引物BOX優(yōu)于引物ERIC。綜合BOXPCR及ERIC．PCR聚類分析，遺傳距離為O．5時(shí)，云南、貴州、廣西、廣東、湖南、江西、福建的部分菌株與A菌系標(biāo)準(zhǔn)菌株的遺傳距離較小，推測這些省份的菌株均為A菌系。云南、浙江、廣東寄主為紐荷爾、湖北的菌株遺傳距離比較大。云南省內(nèi)部菌株之間遺傳距離較大，這可能由于云南復(fù)雜的地理環(huán)境及品種調(diào)運(yùn)引起。6．對(duì)24種藥劑進(jìn)行室內(nèi)毒力測定，噻霉酮、農(nóng)用硫酸鏈霉素及溴菌睛的效果較好，噻霉酮的毒力優(yōu)于農(nóng)用硫酸鏈霉素和溴菌睛。選擇10種潛力較好藥劑進(jìn)行田間藥效試驗(yàn)，結(jié)果表明春雷霉素400倍液、農(nóng)用鏈霉素1000倍液、噻霉酮900倍液、琥膠肥酸銅600倍液對(duì)柑桔潰瘍病防治效果較好，其中噻霉酮和琥膠肥酸銅為化學(xué)試劑，推薦在生產(chǎn)中使用生物制劑春雷霉素和農(nóng)用鏈霉素。關(guān)鍵詞柑桔潰瘍病菌；菌系；REPPOR；遺傳多樣性；防治Ⅱ

簡介：SCREENINGOFBIOCONTROLSTRAINANDSTUDYOFTHECONTROLEFFECTONPEARRINGROTBYDINGFANGBINGSUPERVISEDBYPROFESSORCHENZHIYIATHESISSUBMITTEDTONANJINGAGRICUTURALUNIVERSITYINPARTIALFULFILLMENTOFTHEREQUIREMENTSFORTHEMASTERDEGREEOFSCIENCEDEPARTMEMOFPLANTPROTECTIONNANJINGAGRICULTUREUNIVERSITYNANJING210095，PRCHINAJUNE，2009原創(chuàng)性聲明本人鄭重聲明所呈交的學(xué)位論文，是本人在導(dǎo)師的指導(dǎo)下，獨(dú)立進(jìn)行研究工作所取得的成果。除文中已經(jīng)注明引用的內(nèi)容外，本論文不包含任何其他個(gè)人或集體已經(jīng)發(fā)表或撰寫過的作品成果。對(duì)本文的研究做出重要貢獻(xiàn)的個(gè)人和集體，均已在文中以明確方式標(biāo)明。本人完全意識(shí)到本聲明的法律結(jié)果由本人承擔(dān)。學(xué)位論文作者需親筆簽名丁芳兵2口哆年多月，1EL學(xué)位論文版權(quán)使用授權(quán)書本學(xué)位論文作者完全了解學(xué)校有關(guān)保留、使用學(xué)位論文的規(guī)定，同意學(xué)校保留并向國家有關(guān)部門或機(jī)構(gòu)送交論文的復(fù)印件和電子版，允許論文被查閱和借閱。本人授權(quán)南京農(nóng)業(yè)大學(xué)可以將本學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存和匯編學(xué)位論文。保密口，在年解密后適用本授權(quán)書。本學(xué)位論文屬于不保密∥請(qǐng)?jiān)谝陨戏娇騼?nèi)打“√“學(xué)位論文作者需親筆簽名丁等兵導(dǎo)師需親筆簽名了搬21年歹月，ELZ口聽年石月日

簡介：西北大學(xué)博士學(xué)位論文蘋果早期落葉病生物防治關(guān)鍵技術(shù)研究及其田間應(yīng)用姓名陳亮申請(qǐng)學(xué)位級(jí)別博士專業(yè)微生物學(xué)指導(dǎo)教師陳五嶺20090604ALTERNARIAALTERNATAFRIESKEISSLER和ALTERNARIASOLANI等病原真菌和ESCHERICHIACOLI均具有抑菌活性，其中對(duì)ALTERNARIAALTERNATAFSPMALI、MARSSONINACORONARIAELLETDAVISDAVIS的EC50分別為5。03GG／ML和560IXG／ML。BSP具有良好的熱穩(wěn)定性、PH穩(wěn)定性和蛋白酶穩(wěn)定性。菌株BS24可分泌胞外蛋白酶。綜合考慮工業(yè)化生產(chǎn)要求，以發(fā)酵液活菌數(shù)為檢測標(biāo)準(zhǔn)，從6種供試發(fā)酵培養(yǎng)基中選出初始發(fā)酵培養(yǎng)基。采用PLACKETTBURMAN設(shè)計(jì)法、最陡爬坡試驗(yàn)、中心組合設(shè)計(jì)法進(jìn)行進(jìn)一步的優(yōu)化，得到擬合良好的回歸方程，R2O96。模型驗(yàn)證顯示預(yù)測值與實(shí)測值之間具有較好的擬合性，預(yù)測模型可靠。未優(yōu)化前發(fā)酵液活菌數(shù)為86109CFU／ML，優(yōu)化后為1508X109CFU／ML，增加了7535％。優(yōu)化后發(fā)酵工藝為淀粉15G，黃豆粉216G，酵母膏03G，蔗糖O5G，尿素O2G，MNS04001G，MGS04001G，K2HP0402G，蒸餾水100ML，PH70，轉(zhuǎn)速300R／MIN，接種量78％。田間試驗(yàn)結(jié)果表明，拮抗菌劑三個(gè)不同濃度的處理均對(duì)蘋果早期落葉病有較好的防治效果，拮抗菌劑原液、拮抗菌劑50倍液和拮抗菌劑100倍液防治效果分別為8314％、7921％和7699％，均顯著高于10％多抗霉素WP1000倍的7004％P005。供試拮抗菌劑在試驗(yàn)濃度范圍內(nèi)對(duì)供試果樹無任何不良影響，使用安全。拮抗菌劑處理組果實(shí)感官評(píng)價(jià)、農(nóng)藥殘留、重金屬殘留均符合國家無公害食品的要求。關(guān)鍵詞節(jié)果早期落葉病，生物防治，枯草芽孢桿菌，巨大芽孢桿菌，抑菌多肽，發(fā)酵工藝優(yōu)化，響應(yīng)面分析，田間試驗(yàn)H

簡介：新疆農(nóng)業(yè)大學(xué)碩士學(xué)位論文嗜線蟲真菌DUDDINGTONIAFLAGRANS培養(yǎng)技術(shù)及其對(duì)綿羊胃腸道線蟲病的防治試驗(yàn)姓名王琳申請(qǐng)學(xué)位級(jí)別碩士專業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師岳城王治才20060601嗜線蟲真菌D“捌婦科刪抽砌GM料培養(yǎng)技術(shù)及其對(duì)綿羊胃腸道線蟲病的防治試墮中文摘要嗜線蟲真菌砌蒯，力耵∞，毒F，置∥鯽S培養(yǎng)技術(shù)及其對(duì)家畜胃腸道線蟲病的防治試驗(yàn)嗜線蟲真菌刪坩T∞婦爿AG描門S是迄今發(fā)現(xiàn)并研究證明最具有家畜胃腸道線蟲病生物防制潛力的真菌。通過對(duì)該真菌的培養(yǎng)，制備大量的厚垣孢子，用于家畜胃腸道寄生蟲病的生物防治已取得明顯效果。本試驗(yàn)對(duì)引進(jìn)的仇伽船F(xiàn)伽厶訂酋船冊菌株進(jìn)行了培養(yǎng)基篩選、厚垣孢子生產(chǎn)技術(shù)及菌種保存、制種、發(fā)酵和擴(kuò)大培養(yǎng)等方面的研究，并用培養(yǎng)生產(chǎn)出的該真菌制劑對(duì)綿羊胃腸道線蟲病進(jìn)行了田間防治試驗(yàn)。1根據(jù)價(jià)格便宜、來源方便、制備簡單、厚垣孢子生成多的原則，確定小麥為嗜線蟲真菌口刀矗GR鯽S的固體培養(yǎng)基質(zhì)、5％馬鈴薯汁或玉米汁作為液體培養(yǎng)基的配方最佳。2嗜線蟲真菌口爿刪S厚垣孢子最適生長溫度為25～30℃、培養(yǎng)基最適PH范圍為557O；作為需氧真菌，口F冶GRA口占最合適的培養(yǎng)基質(zhì)裝量W為培養(yǎng)容器容積的022024，即250毫升三角瓶裝小麥60克左右。3低溫冷凍可長期保存嗜線蟲真菌口爿丑LG聊描菌種。將該真菌C姒瓊脂培養(yǎng)物裝入含20％甘油PBS的EPENDORF管中凍存于液氦中，至少可保存78個(gè)月。將菌種直接凍存于一25℃可保存48個(gè)月。該真菌WA、CMA、PA瓊脂培養(yǎng)平板用PARFILM膜封口后置4℃冰箱可保存12個(gè)月。4PA、CMA和WA瓊脂平板可作為該真菌一級(jí)種子的良好培養(yǎng)基。該真菌固體二級(jí)種子以250毫升三角瓶為培養(yǎng)容器，配方為小麥60克、5％馬鈴薯汁或玉米汁48毫升，厚垣孢子數(shù)可達(dá)5105以上。該真菌擴(kuò)大培養(yǎng)采用PVC塑料袋為發(fā)酵培養(yǎng)容器，成本低，方便實(shí)用，可滿足大量生產(chǎn)，孢予數(shù)達(dá)5105以上。5用上述方法生產(chǎn)出的厚垣孢予以51曠／KG體重的劑量作為飼料添加劑，對(duì)伊犁地區(qū)圍欄草場放牧的綿羊進(jìn)行胃腸道線蟲防制的田間試驗(yàn)。結(jié)果表明，該真菌可使放牧草場中三期幼蟲較對(duì)照組減少826％；糞便EPG較對(duì)照組減少873％。表明用該技術(shù)生產(chǎn)的厚垣孢子具有良好的生物防治性能，能夠用于放牧家畜胃腸道線蟲病的防治。關(guān)鍵詞嗜線蟲真菌，厚垣孢子，培養(yǎng)技術(shù)，胃腸道線蟲，生物防治6