15471.極譜分析法在涎磷蛋白和氧化石墨烯電化學(xué)檢測(cè)中的應(yīng)用

1、極譜分析法在涎磷蛋白和氧化石墨烯電化學(xué)檢測(cè)中的應(yīng)用學(xué)位論文完成日期指導(dǎo)教師簽字答辯委貽成員粹主盔魚邋d!里鏨盞李坳APPLICATION0FELECTROCHEMICALFORTHESENSITIVEDETECTl0NOFDETINSIALOPHOSPHOPROTEINANDGRAPHENEOXIDEABSTRACTInthispaperasensitiveelectrochemicalenzyme—linkedimmunoassayw

2、asproposedforthedetectionofdetinsialophosphoproteinbyusinghorseradishperoxidase(HRP)asthelabeledenzymeByusingthe33’，5，5Tetramethylbenzic(TMB)，otilidine(OT)，o—phenylenediamine(OPD)，o—dianisidine(ODA)reactionasthesubstrate

3、，theenzymaticcatalyticreactionproductcanbeelectrochemicaldetectedbythesecondorderderivativelinearsweeppolarographywithhighsensitivity，whichwasexhibitedasensitivesecondorderderivativelinearsweeppolarographicreductionpeakT

4、hiselectrochemicaldetectionofDSPPwasfurthercoupledwithasandwichELISAprocedureforthedetectionofDSPPDSPPisanimportantmarkeroforthodonticrootresorptionSoanewsensitivemethodoftheelectrochemicaldetectionoftraceDSPPcouldbeesta

5、blishedThispaperreportthatcarboxyl—modifiedgrapheneoxide(GO—COOH)hasPeroxidaselikeactivitythatcancatalyzethereactionofperoxidasesubstrate33，5，5tetramethylbenzidine(TMB)inthepresenceofH202toproduceabluecolorreactionThegen

6、eratedproductexhibitedasensitivesecondorderderivativelinearsweepvoltammetricreductionpeakinBrittonRobinsonbufferGO—COOHhasevenhighercatalyticactivitytoTMBthanthenaturalenzymehorseradishperoxidase(HRP)Thisproposedmethodwa