真鯛野生種群和人工繁殖群體遺傳多樣性研究.pdf

1、青島海洋大學(xué)碩士學(xué)位論文真鯛野生種群和人工繁殖群體遺傳多樣性研究姓名：孟憲紅申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：海洋分子生物學(xué)指導(dǎo)教師：孔杰2000.6.1ABS’I’ItAC’l’TheGeneticDiversityintheWildPopuIationandHatcheryStockofRedSeabreamQ缸擴(kuò)嬲小巧D，)Tbevaluatemegeneticdiversityofredse孫bream(，’q眇淞聊c扣，)，raIldo

2、mamplifiedpolymo印hicDNA(凡”D)aIldiso巧metecllIliquewereemployedtodetectmegeneticV砸ationofmetwodiffbrentpopulationsBym廿Dtechnique，met、vop叩ulationswerebomrepresentedby23individualsAmong20mdomprimersin0peronKitKfIorarnplifica

3、tionofgenomicDNA，16couldpmducedistinctiVeandreproducibleflngerprintsThetwopopulationsobta協(xié)ed131aIld123bandsrespectively，82a11d67bandswerepolymo印hica11dthemeanproportionsofpolymorphicamplifled矗agmemsofme似opopulationswere6

4、260％and5447％respectiVely’while，t11eavemgehetero巧gosityvaluedO4786and03633respectiVely，nulDu曲isozymetechnique，me鉚opopulationswererepresemedby50individuals，20lociweredetecteda11dthegeneticVariationindexwascalculatedandaIla

5、lyzed，t11epercentageofpolymorphiclociwas45％、35％(尸盯)and50％、45％(P如)respectiVely，memeanobseⅣedhetero巧gosi趴戰(zhàn))wasO1410044and00950043，memeanexpectedheterozygosit)，(H。)waso156o049a11do132o047，t11emealle腩ctivenumberofalleles(瓦)w

6、as1267O105a11d12200118，hetero巧90tedeficiencywasexistedwimmeallheterozygotedeficienc)’index(D)beingO092andO252respectiVely111eresultsoft11et啪techniquesindicatedmehighergeneticdiVersit)rint11isspeciesandmegreatpotentialinp

7、iscatorialgeneticimpmVementThus，the10werresultofhatcherystockt圭lallwildpopulationirnpliedmattleresponsiblehusballd巧a11dmajlagemcntmeasuresmustbetakensoastoenabletllesustamabledeveloDmentoft|lemariculturebymeaIlsofavoidin