江油附子離體培養(yǎng)的初步研究.pdf

1、Y928359镕《：—籃—正一四川師范大學碩士學位論文江油附子離體培養(yǎng)的初步研究李路娥培養(yǎng)單位———_巴山也瑾型當型蘊哇鹺堂雎——一指導教師———曼凸垃—一職稱——』啦蛙L一三匱生職稱——j虹j立—一專業(yè)名稱———魚一—胞—』已—韭—』生———一研究方向———組——』趾—二已——程———一授予學位日期王』—旦_jL生—』—且————一四川師范大學碩士學位論文PrimaryStudyinvitroCultureofRadixAconiti

2、LateralisPreparatainJiangyouMajor：CellBiologyMScCandidate：LilueSupervisor：MaDan—weiWangYanhuaAbstract：RadixAconitiLateralisPreparataiSafamoustraditionalChinesemediciⅡa王materialscomefromSichuanProvincetheplantresourcesofw

3、hichareAconitumcarmichaeliDebxIthasbeenwidelyusedinclinicoftheTradffionalChineseMedicineInrecentyears，RadixAconitiLateralisPreparatahasbecomeamedicinalrawandprocessedmaterialinindustryfromtraditionaltabletandhasabigannua

4、ldemandiIlChinaTheactiveingredientsareobtainedbyinvitropropagationandlargescalecellcultureofRadixAconitiLateralisPreparatacallmeetfuturemaIkctdemandshvitrocultureofRadixAconitiLateralisPreparataiscarriedoutinthisstudy“Ih

5、emsullsmeas旬llowsStudyonplantletsr昭印既把：themed劬nMS6一BA20mgL1NAA03mgL1isusedtoinducestemsegmentsandterminalbuds，themediumMSKTlmgL12，4D2mgL1№執(zhí)03mgL1isusedtoinducecallusfromfibrousroot，theditierentiatebudsmediumisMS6BA20mgL1

6、2，4D2mgL1NAAO3mgL1TheproliferationbudsmediumisMS6BA20mgL1NAA0imgL1：Treatmentonthemedium1／2MSIBAlmgL1KT05mgL1isthebestforrooting；Studyoninducingcallus：differentexplantsareusedtoinducecallusTheresultsshowsfibrousrootsareth