上海市雜草葎草種群遺傳多樣性與遺傳分化研究.pdf

1、2006屆研究生碩士學位論文Y899學校代碼：10269學號：YS03165012摹泉吁苑天擎上海市雜草律草種群遺傳多樣性與遺傳分化研究院系：邃握皇巫撞堂隧專業(yè)：生查堂研冗方向：筮王生查指導(dǎo)教師：睦41勇碩士研究生：墓越玨2006年5月完成摘要Abstract爿j棚“，淞Jc硎比一sisoneofthem萄orannualdioeciousweedsfoundthroughChinaTenpopulationand381lndividu

2、alsinShan曲aiweresampIeda|ldgenotypedusingmicrosatellitesteveal出egenetjcstructure肌dtoprovidesuggestionsonitscontrol_Afterscreenng62primersofH岫州，琊，7primersthatproducedreproducibleandmultiplealleleswereselectedtoamPlifyDNAs

3、ampIesofall上m坍“淞s。a，瑚knJpoPulationsAtotalof47di彘remSSRaelesweredetectedTheperce小agesofpolymorphiclociineachpopulanonwerelOO％inP100leVelandalmost100％inP95level(excepttheFxandQPpopulation)AtpopuIationlevel，me鋤n啪berofa11ele

4、sperlociwas564nIeaverageofexpectedhetem巧gositywaSO5898，theaverageofobVersehetero糾gositywas04391Duetothedeficiencyofheterozygotes，positiVeinbreedingcoef玎cientswercfoundineachpopulationAssumingHardyWeinbe唱equiltbrIum，theav

5、eragegenedivers時w船estimatedtobe0600wilhinthepopulation(HE)，卸d0635atspecieslevel(HT)Thecoe伍cientofgeneticdi腩rentiationbetweenpopulations(GsT)of且s刪2出瑚w踮0，055，indicatingthatmostofthegeneticdiVersityw夠withinpopulationsThegen

6、eticdistanceofpopulationsw骶from02789toO0096，andtheavemgewas01097Thegeneticidenttyw船from07566to09904，andtheaveragewasO8981Thosedatasu鵲estedthatthetenHs∞，托據(jù)珊populationhadhighgeneticidentitya11dIowgeneticdi彘rentiation，which

7、maybeduetothesexualrepmductiOnanddioeciouscharacteristicofHsc口，d鋤sM粕teltes乜ofrelationshipbetweengeographicandgeneticdistancematricesindicatednosign訪cant∞lationshipbetweengcneticdjst卸ceandgeogr印hicaldist繃ce(r=O0784，p=0667

8、)，Hi曲gcneticdive“ityand10wgeneticdi仃erentiationofHs∞，眈瑚inshan曲aisuggestedthac、Vereofmultipleorigins姐ddispersalHighgeneticdiversityhintsthatbi0109icalcontrolofthisweedseemsdi伍cultandchemicalorphysicaImemodsshouldbeutilize