擬南芥snc1相關突變體調控基因的確定及抗病功能研究.pdf

1、湖南農業(yè)大學碩士學位論文擬南芥snc1相關突變體調控基因的確定及抗病功能研究姓名：朱兆海申請學位級別：碩士專業(yè)：細胞生物學指導教師：洪亞輝張躍林20070501AbstractInnateimmunityiscriticalforsensinganddefendingagainstmicrobialinfectioninmulticellularorganismsPlantshaveevolvedsophisticateddefense

2、mechanismsagainstpathogeninfections，duringwhichdiseaseresistancegene(R—genes)playcentralrolesinrecognizingpathogensandinitiatingdownstreamdefensecascadesInArabidopsis，adominantmutantsuppressorofnprl一五constitutive1(sncl)，

3、wasidentifiedpreviouslythatconstitutivelyexpressespathogenesis—related(艘)genesandresistanceagainstbothPseudomonassyringaepvmaculicola僻＆艦)Es4326andPeronosporaparasitica(印)NOC02ItisprovedthatsnclwhichisoneoftheRgenesattheu

4、pstreamoftheresistancepathwayc卸constitutiveactivationofdiseaserealstanceAGRl4isoneoftheAGRseriesmutantsonthebackgroundofsnclmutantbyTDNAinsertionactivationtaggingBymutantsscreeningandplasmidrcs(：Ileforgenelocationtwoknoc

5、koutgenesinAGRl4havebeenidentitledwhiehcanrecoverythephenotypeofsnclandtheirfunctionsareunknownTheregearchcontentsandresultsofthispaperasfollowing：ltisconfirmedthattheAGRl4isdoublemutantofBGandSGgeneonthebackgroundofthes

6、nclmutantbyusingthemethodsofPCRorR1二PCRonthelevelofDNAorRNArespectively弧emumfionoftheBGgene啪turnthephenotypeofsnclbackintoCol一0partiallyratherthantheSGgenebyusingthecomplementationexperimentItprovesthatBGgeneplaysallimpo

7、rtantroleinthephenotypeofthemutantAGRl4whoseinactivation啪suppresstheSNClgenepartiallyTheoverexpressionexperimenttoallalysisthefunctionoftheBGproteinfurthermole，theresultsshowthatBGoverexpressioninCol0canchangethephenotyp

8、eoftheplantsneexpressionoftheBGgeneencodedprotein／nvivoor／nvitrespectivelysuggeststhattheputativeproteinisexistedinArabidopsiswhichischaracterizedasasolubleandhighmolecularweightproteinItispredictedthatthefunctionofthisp

9、roteinactsasatranscriptionalcorepressormediatingproteinproteininteractionbyWD40repeatdomainattheC—terminalanddimerizationoftheLisHstructuredomainattheNterminalaccurdingtotheanalysisofbioimformaticsTheBGGFPfusedgeneexpres

10、sionvectorrecombinanthasbeenconstructedandtransformedintotheplantforencodedproteinsubcellularlocalizationTheresultsshowthatBGislocalizedinthenucleusofplantcellsandpredictedfunctionactasaputativetranscriptionalcorepressor

11、ThebacteriaandfungalinfectionexperimentsandthechangeofthePRgeneontranscriptionallevelshowthatAGRl4wasconstitutivelyexpresses尸Rgenesandmoreresistanceagainstboth只置艦ES4326andPpNOC02thanC010butlessthansnclFurthermoretheresis

12、tanceoftheBG—overexpressingplanttoepNOC02isassociatedwithincreasedexpressionofthesalicylate—regulatedPRJgeneItsuggeststhattheBGgeneisrelatedwiththediseaseresistancefunctioninplantsKeyWords：Arabidopsisthaliana；Complementa