新型固相萃取劑的合成及表征.pdf

1、天津理工大學(xué)碩士學(xué)位論文新型固相萃取劑的合成及表征姓名：魯玲玲申請學(xué)位級別：碩士專業(yè)：應(yīng)用化學(xué)指導(dǎo)教師：李乃瑄20080101AbstractAtpresent，analysistechnologylikemotortolifeandmedicineterritoryAndchromatographictechniqueispopularanasismethodWhereas，SoildPhaseExtractionappliedtop

2、reprocessingforsamplesismoreandmorepopularThispaperstudiedtwonovelkindofSPEusedtoseparatebiomacromoleculeItisessentialtesearchanovalstationaryextractionagent，andtheworkourteamworkedasfollows：30／zmnonporousUreaFormaldehyd

3、eResinZirconiacompositemicrospheres(UFZr02)asthematrixofHighLiquidAffinityChromatographywhichwascharacterizedbyScanningElectronMicroscope(SEM)AmidobondonthesurfaceofthematrixactivatedbyMichaelAdditionReactionwithmethylac

4、rylate，andsubsequentlywithdiaminoethane，thenrepeatedthreetimesAtlast，PAMAMwitharborescencestylewaspreparedThen，thesurfaceofPAMAMweremodifiedbyN，NCarbonyldiimidazole(CDI)，thenchelatedbyBSAsuccessfullyFinallyBSAUFZr02stati

5、onaryextractionreagentWaspreparedItsligandholdingcapacityWastestedusingultravioletspectrophotometricmethodTakingalbumineggandlysozymeastestingsamples，theabsorptivecapacityofBSAUFZr02werestudiedfromabsorptivetime，pHvalue，

6、eluentreagentandconcentrationofsolventTheresearchshowedthatthebestfactorsasfollows：4handpH60foradsorptionoflysozyrae，6handpH60foradsorptionofalbumineggTheMaxadsorptioncallreach5361mg／gforlysozyme，3601mg／gforalbumineggThe

7、Maxelutingrateforlysozymecanreach7326％with05mol／LNaCl，7668％withO125mol／L的KSCNAndtheelutingrateforalbumineggcanreach7805％with05mol／LNaCI，8307％under025mol／LKSCNOntheotherhand，LphenylatanineUFZr02wasproducedforfurherresearc

8、hBovineserumalbumin(BSA)，lysozymeandalbumineggwereusedasthetestsampleWealsoinvestigatetheperformanceofLphenylatanine—UF2102compositemicrospheresviachangingthepHvalues，proteintested，eluentreagentandconcentrationofsolventT

9、heresearchshowedthatthebestfactorsasfollows：6handpH60foradsorptionoflysozyme，6handpH60foradsorptionofalbumineggAdsorptionofalbumineggcanreach3617mg／g，5294mg／gforlysozymeTheelutingratecanreach7162％forlysozymewith025mol／LN

10、aCI，7639％with025mol／LKSCN，and7663％foralbumineggwith025mol／LNaCI，9052％forthatwith0125moI／LKSCNKeywords；Urea—FormaldehyderesinZirconia∞Fzr02)compositemicrospheres，Stationaryextractionreagent，Bovineserumalbumin(BSA)，Lysozym