BBP對斑馬魚的毒性效應(yīng)研究及斑馬魚AChE基因的克隆與表達(dá).pdf

1、⑧碩士學(xué)位論文MASTER‘5Tl于ESISToxicEffectsofButylBenZylPhthalateonZebrafishandCloning，ExPressionofAcetylcholinesteraseGeneAThesisSubmittedinPartialFulfillmentoftheRequirementsFortheM.S.DegreeinBioehemistryandMoleeularBiologyyBZh

2、angCanPostgraduateProgramCollegeofLifeSeienceCentralChinaNormalUniversitySuPervisor:XiongLiACademicTitle:ASS0ciateProfessorSignaturAPril，2011⑧碩士學(xué)位論文MASTER一5TIJESISAbstractAsPlastieizers，softeners，eariersandadditives，BBPa

3、rewidelyusedinPlasties，automotive，lubrieants，eosties，elothing，Pestieideandotherindustries.Beeanseits場drolysis，photolysisandevapationrate15ve巧slow，BBPhasbeeneoneemedasamajofglobalenvironmentalPollutant.AtPresent，theEU，the

4、Unitedstates，JapanothercountrieshasrankedthiseomPoundamongthePriorityeontrolPollutants，ItwasrePresenied“TheseeondglobalPCB印olyehlinatedbiPhenyls)Pollution，，.It15rePtedthatBBPhasobvioustestieletoxieity，teratogenieityandem

5、bryotoxieity，iteanleadserumdeereaseintestosterone，andPromotetheinereaseoffolliele一stimulatinghormone飾sterauxesis，hasehangesinsexualdifferentiation.Inaddition，iteaneanseallergiesandasthma，haveanimPaetonthehumannervoussyst

6、em.It15signifieanttostu勿itsPossibleadverseimpaetong耐sms.Inthisstu勿，fishacutestatietestwasusedtoexPlethetoxiceffeetsofBBPonzebrafish.Basedontheacutetoxieitytest，96hoursmedianlethaleoneentration(LCso)ofBBPe價(jià)etedonzebranshw

7、asdeterminedtobe3.gsmgL一l，Then，zebranshwereexposedtodifferentBBpeoneentrations(0、0.332、0.665、1.33mgL一l).TheAe隴，SODCATactivitiesweremeasuredinthefisheolleetedat7d，14dZldand28dPost一exPosure.Itshowedthatfromodto28d，AChEaeti

8、vitywassignifieantlyinhibited:SODaetivityinereasedfirstlyandthendeelinedwithtimeextension，andwasaetivatedtothehighestlevels(13.51，14.72，11.81，10.19Umg)at14dfallthetreatments.CATaetivity，in7d，exe叩t0.665mgL一’gro叩，wasi汕ibit

9、edsignineantly(P0.0一)，therestgrouPswereofnosignifieantdifferenceeomParedwiththeeontrol.Semi一quantitativeRT一PCRwasusedtodeterminethemRNAtranseriPtlevelsofAChE，SODandCYPIAgeneinbrainandmuseleofzebrafishexPosed7dindifferent

10、eoncentrations(o、0.332、0.665、1.33mgL一’)，Itshowedthat止em即AtranseriptofAChEgenewasdown一regulatedbyBBPexPosurebothinbrainandmuscle:thiswaseonsistentwiththeAChEactivitymeasuredbefe.ThemRNAtranseriPtofSODgeneinzrbra五shbrainwe

11、refirstdeereasedandreboundedmodestlyat1.330mgL一1group.xnzrbrafishmusele，themRNAtranseriPtofSODgenedisPlayedaninhibitiontrend，eomParedwiththeeonirol，theinhibitionrateswere19.33%，17.86%，17.23%.ThebrainmRNAtranseriPtofCYPIA