微絲動態(tài)調(diào)控植物細胞頂端生長以及AtSNAP33蛋白在擬南芥根毛細胞中的分布與作用.pdf

1、中國農(nóng)業(yè)大學博士學位論文微絲動態(tài)調(diào)控植物細胞頂端生長以及AtSNAP33蛋白在擬南芥根毛細胞中的分布與作用姓名：何雪申請學位級別：博士專業(yè)：植物學指導教師：李巖20070601AbstractAetindistributioninroothairtipsisacontroversialtopicAlthoughtherelationshipIⅪatweenCazgradientandaetindynamicsinplanttipgrow

2、thhasbeenafocusofstlldythereisstilllittledirectevidenceOiltheexactrelationshipinroothair印growth(3aetinWalllabeledbyfluoreseeinisotlaioeyamteDNaseLFaetinWalllabeledbyte自御ethyI而odaminei如曲io印鋤mte呻halloidimActininroothairsof

3、Tritieumaestivum(wheat)wasinvestigatedusingeonfoeallaserscanningmicroscopyThickFactinbundlesdidnotextendintoaregionofapprox5一lOrainfromthetipofthegrowinglOOthairsalthoughtheygaveoffbranehesoffineactillfilamentsinthehair卸

4、sAtipfocusedGaetingradientw∞shownattheextremeap％ofgrowingroothairsInfullgrownwheatroothairs，thetipfocusedGaetingradientdisappearedwhilethe也ickl3aetinbundlesextendedintothetipsBAPTAAMaCa2disnlptionagentalsocausedthetipfoc

5、usedGaetingradienttodisappeⅡandthediffuseFactinbundlestoappeal“inthetipsofwheatroothairsTheseresultssuggestthatthetipfocusedgradientofiatraeellularGaetinCOncentrationattheextremoapexmaybeessentialforroothairgrowthandthat

6、preservingthetipfocusedgradientn∞dsahighCazconcentrationintheroothairtipsRecentstudiesindicatedthatdyaamiesoftiplocalizedatriacontrols印growthinpollentubesThefusionbd3tw∞n∞cI吲ofyvesiclesandthemembraneinthetippushesthepoll

7、entubegrowthExaminationofthedynamicsofFMl43／464labelinginthepollentubeallertreatingbythedrugsofaetindypolymerizationandaetinpolymerizationsuggestedthevesicleshealthemembraneWaSdisappearanceandpollentubegrowthWaSinhibited

8、Toexplaintheeffectofaetin‘lynamiesOndistributionofapicalvesiclesWequantitativelyanalyzedtheintensityofFM464∞dABD2GFPintheapexThesedatarevealedthetiplocalizedvesicleso∞illatesinthesamephasewiththevelocityofpollentubestipg

9、rowthandintheOplmiewiththeFactinfluorescentintensityTheseresultssuggestthatpollentubegrowthisrelatedtodynllmicdistributionofvesicleswhichiscontrolledbytiplocalizedactinIneukaryotiecellsthespecificmembraneftlSiOl3sa∞media

10、tedbyafamilyofSNAREproteins68圈吼虹通proteinsWerealsofoundinArabiaopsisAnalysisresultofchipsdisplayedthattheexpressionofSNAREsshowdifferentlevelinvariousorgansatdifferentdevelopmentstagesinArabidopsisThesedifferentSNAREsmayb

11、einvolvedindiveasemembranefusionprocessinvariousorgansatdifferelltdevelopmentstagesinplantcellsRoothairisaspecial位pgrowthcellandtheSNAREscomplexwhielai8involvedinmembranofusion班oeessinroothairtipsWallnotrepOl缸Inhereimmun

12、olocalizationofSNAP33proteinand衄msgenieArabidopsisplantsofSNAP33GFIallshowthattlaeSNAP33localizemostlyintheroothairstipQverexpressin蝌盤33genecausedfasterhairgrowthandinolelOOthairsInadditionoverexpre∞seedlingsdisplayedles